Applied Biosystems™
TaqMan™ Protein Expression Assay Kit (hCSTB)
Applied Biosystems™ TaqMan™ Protein Expression Assays enable fast, easy identification, and relative protein quantification of pluripotency protein markers from limited Read more
Catalog number 4405465
Price (USD)/ 100 reactions
491.00
-
Price (USD)/ 100 reactions
491.00
TaqMan™ Protein Expression Assay Kit (hCSTB)
Catalog number4405465
Price (USD)/ 100 reactions
491.00
-
Applied Biosystems™ TaqMan™ Protein Expression Assays enable fast, easy identification, and relative protein quantification of pluripotency protein markers from limited quantities of cultured hESCs. The TaqMan™ Protein Expression Assay Kit (Human CSTB) contains the assay probes and a dilution buffer for the labelling of human cystatin-B protein (hCSTB) in cell lysates prepared using the Protein Expression Sample Preparation Kit. The assay probes consist of two oligonucleotides conjugated to antibodies that bind to separate epitopes of the hCSTB protein. Key features of the TaqMan™ Protein Expression Assay:
• Quantitates protein expression using gold standard TaqMan™ 5’ nuclease chemistry
• Enables direct correlation of mRNA and/or miRNA expression to protein expression on the same real-time PCR platform
• Allows greater sensitivity and a more quantitative readout than western blots and immunostaining
• Quantitates protein expression using gold standard TaqMan™ 5’ nuclease chemistry
• Enables direct correlation of mRNA and/or miRNA expression to protein expression on the same real-time PCR platform
• Allows greater sensitivity and a more quantitative readout than western blots and immunostaining
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodPrimer-probe
GC-Rich PCR PerformanceLow
PCR MethodqPCR
PolymeraseAmpliTaq Fast DNA Polymerase
Reaction SpeedFast
TechniqueProximity Ligation Assay
For Use With (Equipment)7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
Green FeaturesSustainable packaging
Label or DyeFAM
Passive Reference DyeROX (Pre-mixed)
Product LineTaqMan™
Product TypeProtein Expression Assay Kit
Purity or Quality GradeUP (Ultra Pure)
Shipping ConditionWet Ice
Sufficient For100 Reactions
Target SpecificityTarget-Specific
Hot StartNo
No. of Reactions100 reactions
Sample TypeCells
Unit Size100 reactions
Contents & Storage
Contains Assay Probe A, Assay Probe B, and Assay Probe Dilution Buffer. The assay probes (Assay Probe A and Assay Probe B) are supplied at a 20x concentration. The Assay Probe Dilution Buffer is supplied at a 1x concentration. Store at -15 to -25°C.