BigDye™ Terminator v1.1 Cycle Sequencing Kit
Notice: We're enhancing our manufacturing process for DNA Control Template, now labelled as pREF-BDT Control DNA in kit, for consistent high-quality delivery. No changes to preparation or storage conditions will occur ensuring seamless integration into your current workflows. Please see the product user guide for more information.
BigDye™ Terminator v1.1 Cycle Sequencing Kit
Applied Biosystems™

BigDye™ Terminator v1.1 Cycle Sequencing Kit

This BigDye™ Terminator v1.1 Cycle Sequencing Kit is designed for specialty applications that require optimal basecalling adjacent to the primer  Read more
Have Questions?
Change viewbuttonViewtableView
Catalog NumberQuantity
433744924 Reactions
4337450100 Reactions
43374511000 Reactions
43374525000 Reactions
Catalog number 4337449
Price (USD)/ 24 reactions
-
Request A Quote
Quantity:
24 Reactions

This BigDye™ Terminator v1.1 Cycle Sequencing Kit is designed for specialty applications that require optimal basecalling adjacent to the primer and for sequencing short PCR product templates with rapid electrophoresis run modules.

  • Improve the quality of your results for a wide range of sequencing applications
  • Sequence challenging templates and sequences more successfully
  • Get longer, higher-quality reads with more uniform peak heights and optimal signal balance
  • Enhance your productivity and reduce costs

Tackle Tough Sequences

With the BigDye Terminator v1.1 Cycle Sequencing Kit you get a robust, highly flexible chemistry for a wide range of applications, including de novo sequencing and resequencing.

Get More Uniform Peak Heights, Improved Accuracy

With BigDye Terminator v1.1 kit's superior chemistry, you generate data with uniform peak heights and optimized signal balance-which results in longer, higher-quality reads. You also get more accurate base assignments for heterozygote and mutation detection.

Easily Integrate into Your Workflow

BigDye™ Terminator v.1.1 kit's chemistry requires no new software or instrument recalibration.You can easily integrate either kit into your current workflow with minimal changes to your protocols.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Equipment)SeqStudio™ Genetic Analyzer, SeqStudio™ Flex Series Genetic Analyzer, 3500/3500xL Genetic Analyzer, 3730/3730xl DNA Analyzer Thermal Cyclers: GeneAmp™ PCR System 9700 Dual Series, Veriti™ Fast 96‐Well and 384‐Well Thermal Cyclers
For Use With (Application)AT-Rich Sequencing (>65%), Comparative Sequencing (Germline Mutations - 50:50), Short PCR Product Sequencing, GC-Rich Sequencing (>65%), GT-Rich or Difficult Template Sequencing, De Novo Sequencing - Low to Mid Throughput
Product LineBigDye™ Terminator
Product TypeSequencing Buffer
Quantity24 Reactions
TechniqueFluorescent Dye Terminator Sequencing
Template CompatibilityBAC DNA, Plasmid DNA (≤15 Kb), PCR Amplicons, Single Stranded DNA
Unit Size24 reactions
Contents & Storage
• 200 μL Tube of BigDye™ Terminator v1.1 Ready Reaction Mix
• Tube M13 (-21) Primer
• Tube pREF-BDT™ Control DNA (200 ng/μL)
• 1 mL Tube of 5x Sequencing Buffer (may be stored at 4°C)

Store kit at -15°C to -25°C, and avoid repeated freeze-thaw cycles.