Thermo Scientific™
SuperSignal™ West Dura Extended Duration Substrate
Thermo Scientific SuperSignal West Dura Extended Duration Substrate is a luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate ideal for Read more
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| Catalog Number | Kit Contents | Quantity |
|---|---|---|
| 34075 | Luminol/Enhancer, 50mL | 100 mL |
| 34075X4 | Luminol/Enhancer, 50 mL | 4 x 100 mL |
| 34076 | Luminol/Enhancer, 100mL | 200 mL |
| 34076X4 | Luminol/Enhancer, 100 mL | 4 x 200 mL |
| 37071 | Stable Peroxide Buffer, 10mL | 20 mL |
Catalog number 34075
Price (USD)/ 100 mL
374.65
Online exclusive
383.00 Save 8.35 (2%)
-
Kit Contents:
Luminol/Enhancer, 50mL
Quantity:
100 mL
Price (USD)/ 100 mL
374.65
Online exclusive
383.00 Save 8.35 (2%)
SuperSignal™ West Dura Extended Duration Substrate
Catalog number34075
Price (USD)/ 100 mL
374.65
Online exclusive
383.00 Save 8.35 (2%)
-
Thermo Scientific SuperSignal West Dura Extended Duration Substrate is a luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate ideal for quantitative western blotting with stable light output for mid-femtogram level detection for western blot analysis.
SuperSignal West Dura Extended Duration Substrate characteristics include:
• Sensitivity: mid-femtogram
• Stability: 24-hr working solution stability; 1-year kit stability at room temperature
• Compatibility: nitrocellulose and PVDF membranes
• Signal duration: 24 hours
• Recommended primary antibody concentration: 1:1,000–1:50,000 dilution (0.02–1.0 μg/mL)
• Recommended secondary antibody concentration: 1:50,000–1:250,000 dilution (4–20 ng/mL)
Compare and view all western blot chemiluminescent substrates ›
SuperSignal West Dura Extended Duration Substrate for HRP is optimized for high sensitivity and long signal duration, making it ideal for cooled CCD camera-based detection systems. Unlike substrates with signals that decline to barely detectable levels in 30–60 minutes, the signal produced with SuperSignal West Dura chemiluminescent substrate is stable for 24 hours, allowing multiple film or camera exposures.
SuperSignal West Dura Extended Duration Substrate characteristics include:
• Sensitivity: mid-femtogram
• Stability: 24-hr working solution stability; 1-year kit stability at room temperature
• Compatibility: nitrocellulose and PVDF membranes
• Signal duration: 24 hours
• Recommended primary antibody concentration: 1:1,000–1:50,000 dilution (0.02–1.0 μg/mL)
• Recommended secondary antibody concentration: 1:50,000–1:250,000 dilution (4–20 ng/mL)
Compare and view all western blot chemiluminescent substrates ›
SuperSignal West Dura Extended Duration Substrate for HRP is optimized for high sensitivity and long signal duration, making it ideal for cooled CCD camera-based detection systems. Unlike substrates with signals that decline to barely detectable levels in 30–60 minutes, the signal produced with SuperSignal West Dura chemiluminescent substrate is stable for 24 hours, allowing multiple film or camera exposures.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product TypeSubstrate
Detection MethodChemiluminescence
FormLiquid
For Use With (Application)Western Blot
Membrane CompatibilityNitrocellulose, PVDF
Product LineSuperSignal™
Quantity100 mL
SubstrateHorseradish Peroxidase
Recommended Antibody Concentrations2°: 1:50-250K (4-20ng/mL), 1°: 1:5K (0.02- 1μg/mL)
SensitivityMid-femtogram
Signal Duration24 hr.
Sufficient For12 Mini-Gel Blots, 1000 sq. cm of Membrane
Kit ContentsLuminol/Enhancer, 50mL, Stable Peroxide Buffer, 50mL
Unit Size100 mL
Contents & Storage
Sufficient For: 12 mini-gel blots; 1000 cm2 of membrane
• Luminol/Enhancer, 50 mL
• Stable Peroxide Buffer, 50 mL
Store at room temperature.
• Luminol/Enhancer, 50 mL
• Stable Peroxide Buffer, 50 mL
Store at room temperature.