Opti-MEM™ I Reduced Serum Medium
| Catalog Number | Quantity |
|---|---|
| 31985062 | 100 mL |
| 31985070 | 500 mL |
| 31985088 | 10 x 500 mL |
Opti-MEM™ I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change in growth rate or morphology. Opti-MEM™ I medium is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM™ I medium can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. We offer a variety of Opti-MEM™ I modifications for a range of cell culture applications.
This Opti-MEM™ I is modified as follows:
| With |
| • L-glutamine |
| • Phenol Red |
The complete formulation is confidential. For more information, please contact Technical Services.
Using Opti-MEM™ I Medium
Opti-MEM™ I Reduced-Serum Medium is a unique medium that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. These additional components allow for a reduction in serum supplementation by at least 50%. Opti-MEM™ I medium uses a sodium bicarbonate buffer system (2.4 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.