Catalog Number | Quantity |
---|---|
2943072010 | 10 mL |
2943072005 | 5 mL |
2943072050 | 50 mL |
1943072250 | 250 mL |
1943072500 | 500 mL |
194307201L | 1 L |
194307205L | 5 L |
CaptureSelect C-tagXL Affinity Matrix combines a unique selectivity for a small 4-amino acid peptide tag (E-P-E-A, glutamic acid-proline-glutamic acid-alanine) with the benefits of a robust and high quality affinity matrix. It purifies C-terminal tagged proteins with high affinity and selectivity, even in the presence of urea and guanidine HCl, from complex mixtures like cell culture harvests and periplasmatic fractions in a one-step process. Mild elution conditions at neutral pH can be applied using magnesium chloride or propylene glycol, which ensures high activity recoveries of pH-sensitive target proteins. C-tagXL Affinity Matrix recognizes the E-P-E-A tag sequence when fused either directly to the C-terminus of a protein or through a linker between the C-terminus and the E-P-E-A tag.
Features of C-tagXL Affinity Matrix include:
CaptureSelect C-tagXL Affinity Matrix purifies C-terminal tagged E-P-E-A proteins directly from complex source materials in a single step with high purity and yield.
Free of animal components
CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).
Main characteristics: