Pierce™ High Capacity NeutrAvidin™ Agarose

Thermo Scientific High Capacity NeutrAvidin Agarose is a beaded agarose resin of immobilized NeutrAvidin Protein, a modified form of avidin with exceptional biotin-binding characteristics for affinity purification methods.

Features of High Capacity NeutrAvidin Agarose:

• NeutrAvidin protein—purified, deglycosylated avidin protein (60kDa, pI 6.3); tetrameric with four biotin-binding site per molecule
• Agarose resin—support is crosslinked 6% beaded agarose (CL-6B), the most popular resin for protein affinity purification methods
• Inert and stable—NeutrAvidin protein is immobilized by charge-free, leach-resistant covalent bonds, resulting in low nonspecific binding and enabling multiple uses without decline in yield
• High capacity—this variety of beads has a dense load of immobilized NeutrAvidin protein, providing a binding capacity greater than 8 mg of biotinylated BSA per mL of resin

High Capacity NeutrAvidin Agarose consists of a specially purified and deglycosylated form of avidin that has been covalently immobilized at a high density onto high-quality crosslinked 6% beaded agarose. Compared to native avidin, NeutrAvidin protein is more neutral and exhibits much less nonspecific binding properties in biotin-binding applications. The agarose beads have physical and chemical properties that enable them to be used in a variety of batch- or column-type affinity procedures. Generally, NeutrAvidin products function as well or better than their streptavidin counterparts and are less expensive.

NeutrAvidin protein is deglycosylated native avidin from egg whites. Removal of the excess carbohydrate by an exclusive process yields a protein with a more neutral isoelectric point and less nonspecific binding properties. NeutrAvidin resins are prepared by covalently coupling the protein using efficient and stable chemistries, resulting in supports that are resistant to leaching and stable at pH 2-11. The products are excellent choices for a variety of small- or large-scale affinity purification applications involving biotinylated macromolecules, including separation of biotinylated molecules from samples and immunoprecipitation of antigens using biotin-labeled antibodies.

Properties of crosslinked 6% beaded agarose (CL-6B):
• Support pH Stability: 2 to 14 (short term); 3 to 13 (long term)
• Average Particle Size: 45 to 165 microns
• Exclusion Limit: 10,000 to 4,000,000 daltons
• Maximum Volumetric Flow Rate: approx. 1 mL/minute (for 1 cm diameter column)
• Maximum Linear Velocity: 30 cm per hour
• Maximum Pressure: less than 25psi (1.5 bar)

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