Pierce™ Bradford Protein Assay Kit
Please note the name of Cat. No. 23200 has changed from "Pierce Coomassie (Bradford) Protein Assay Kit" to "Pierce Bradford Protein Assay Kit". This update is a name change only, no other changes were made to this product.
Pierce™ Bradford Protein Assay Kit
Thermo Scientific™

Pierce™ Bradford Protein Assay Kit

The Pierce Bradford Protein Assay Kit is a ready-to-use, stable formulation of the traditional Bradford assay reagent for measurement ofRead more
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Catalog number 23200
Price (USD)/ 950 mL
143.65
Online exclusive
150.00 
Save 6.35 (4%)
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Price (USD)/ 950 mL
143.65
Online exclusive
150.00 
Save 6.35 (4%)
Add to cart
Pierce™ Bradford Protein Assay Kit
Catalog number23200
Price (USD)/ 950 mL
143.65
Online exclusive
150.00 
Save 6.35 (4%)
-
Add to cart
The Pierce Bradford Protein Assay Kit is a ready-to-use, stable formulation of the traditional Bradford assay reagent for measurement of total protein concentration compared to a protein standard. The kit includes Bradford Protein Assay Reagent and a package of Albumin Standard ampules.

Compare all available Bradford assays ›

The Pierce Bradford Protein Assay Kit is a ready-to-use formulation of the popular assay reagent originally described by Bradford in 1976. When mixed with a protein solution, the acidic Coomassie-dye reagent changes color from brown to blue in proportion to the amount of protein present in the sample. Protein determinations are made by comparison to the color response of protein assay standards, usually prepared as a series of known dilutions of bovine serum albumin (BSA) or bovine gamma globulin (BGG). The simple procedure is adaptable to nearly any volume scale, including test tubes, cuvettes, and microplates. The protein assay is compatible with most salts, solvents, buffers, thiols, reducing substances, and metal chelating agents encountered in protein samples.

Features of the Bradford Protein Assay Kit include:
Bradford reagent—stable, ready-to-use kit of the classical Bradford assay reagent
Colorimetric—measure with a standard spectrophotometer or plate reader at 595 nm
Easy to use—single reagent; no working reagent preparation required
Fast—almost immediate color development; add, mix, and read results
Assay range—detects protein concentration in the range 1 to 1500 μg/mL
Flexible—microplate and cuvette protocols provided and adaptable to several target working ranges

How the Bradford Assay detects protein
Development of color in Coomassie dye-based (Bradford) protein assays has been associated with the presence of certain basic amino acids (primarily arginine, lysine, and histidine) in the protein. Van der Waals forces and hydrophobic interactions also participate in the binding of the dye by protein. The number of Coomassie dye ligands bound to each protein molecule is approximately proportional to the number of positive charges found on the protein. Free amino acids, peptides, and low molecular weight proteins do not produce color with Coomassie dye reagents. In general, the mass of a peptide or protein must be at least 3,000 daltons to be assayed with this reagent.

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WARNING: Reproductive Harm - www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.
Specifications
AssayBradford Assay
Detection MethodColorimetric
Product LinePierce™
Product TypeProtein Quantitation Assay
SpecificityNot Target-Specific
Sufficient For630 Tube Assays or 3800 Microplate Assays
For Use With (Application)Solution-based Detection, Absorbance
For Use With (Equipment)Spectrophotometer, Microplate Reader
Quantity950 mL
Unit Size950 mL
Contents & Storage
Sufficient For:
  • 630 tube assays or 3800 microplate assays
  • Pierce Bradford Assay Reagent, 950 mL
  • Albumin Standard ampules, 2 mg/mL, 10 x 1 mL
Store Bradford Protein Assay Reagent at 4°C. Store unopened Albumin Standard ampules at room temperature.