MAX Efficiency™ DH5α Competent Cells

MAX Efficiency™ DH5α Competent Cells are a well-known, versatile strain that is used in many everyday cloning applications. Prepared by a proprietary modification of the Hanahan method, these cells are suitable for high efficiency transformations in applications where low amount of DNA is used or to construct libraries using plasmid-derived vectors. In addition to supporting blue/white screening, recA1 and endA1 mutations in DH5α cells increase insert stability and improve the quality of plasmid DNA prepared from minipreps.

Benefits of MAX Efficiency DH5α Competent Cells:
• Versatile strain for everyday cloning applications
• High quality plasmid preparations
• Useful for generating plasmid libraries 

DH5α competent cells for high transformation efficiency
The Φ80lacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors, and can be used for blue/white screening of colonies on bacterial plates containing Bluo-gal or X-gal. DH5α cells are capable of being transformed efficiently with large plasmids, and can also serve as a host for the M13mp cloning vectors if a lawn of DH5αF′IQ (or similar) is provided to allow plaque formation.

Features of MAX Efficiency DH5α Competent Cells include:
• Transformation efficiency of >1 x 10⁹ transformants/μg plasmid DNA
• High plasmid yield from the DH5α (endA1) E. coli strain 
• Blue/white screening capable (lacZΔM15)
• Greater insert stability (recA1)

Genotype:
F- Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 (rk-, mk+) phoA supE44 λ-thi-1 gyrA96 relA1 

Find the strain and format that fits you needs
• DH5α strain and its derivatives available in with a variety of transformation efficiencies and in both electrocompetent and chemically competent formats 
• ElectroMAX™ DH5α-E Competent Cells are engineered for electroporation to achieve high transformation efficiency of >1 x 10¹⁰
• DH5α T1R competent cells are available in MultiShot format for high throughput applications