M-MLV Reverse Transcriptase Buffer is for use with Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT).
5X buffer composition
• 250 mM Tris-HCl (pH 8.3)
• 375 mM KCl
• 15 mM MgCl2
Included with
• M-MLV Reverse Transcriptase
• SuperScript Reverse Transcriptase
• SuperScript II Reverse Transcriptase
• SuperScript III Reverse Transcriptase
M-MLV Reverse Transcriptase Buffer does NOT contain actinomycin D, nucleotides, or dithiothreitol (DTT).
• M-MLV Reverse Transcriptase Buffer (1 mL)
• 0.1 M DTT (500 μL)
Store at –20°C.