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Invitrogen™
Ribonuclease H
Ribonuclease H (RNase H) is an endoribonuclease that specifically degrades the RNA strand of an RNA-DNA hybrid to produce 5´Read more
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Catalog Number
Quantity
18021071
120 U
18021014
30 U
Catalog number 18021071
Price (USD)/ 120 units
584.65
Online exclusive
634.00
Save 49.35 (8%)
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Add to cart
Quantity:
120 U
Request bulk or custom format
Price (USD)/ 120 units
584.65
Online exclusive
634.00
Save 49.35 (8%)
Add to cart
Ribonuclease H
Catalog number18021071
Price (USD)/ 120 units
584.65
Online exclusive
634.00
Save 49.35 (8%)
-
Add to cart
Ribonuclease H (RNase H) is an endoribonuclease that specifically degrades the RNA strand of an RNA-DNA hybrid to produce 5´ phosphate-terminated oligoribonucleotides and single-stranded DNA. Applications Removal of mRNA during second-strand cDNA synthesis. Removal of poly(A) sequences from mRNA in the presence of oligo(dT). Oligodeoxyribonucleotide-directed cleavage of RNA. Source Purified from E. coli expressing the E. coli RNase H gene on a plasmid. Performance and quality testing Ribonuclease, nonspecific endodeoxyribonuclease, 3´ and 5´ exodeoxyribonuclease. Unit definition One unit hydrolyzes 1 nmol of RNA in 3H-labeled poly(A)poly(dT) to acid-soluble material in 20 min at 37°C. Unit reaction conditions 20 mM Tris-HCl (pH 7.5), 0.1 M KCl, 10 mM MgCl2 , 0.1 mM DTT, 5% (w/v) sucrose, 0.5 nmol 3H-labeled poly(A)poly(dT), and enzyme in 50 μL for 20 min at 37°C.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration2 U/μL
Shipping ConditionApproved for shipment on Wet or Dry Ice
EnzymeRNase
Quantity120 U
Product TypeRibonuclease H
Unit Size120 units
Contents & Storage
4 vials of Ribonuclease H with each containing 30 units supplied at a concentration of 2U/μl