pMCS-Gaussia-Dura Luc Vector for Luciferase Assays

The Thermo Scientific pMCS-Gaussia-Dura Luc Vector is a multiple cloning site plasmid designed to accept a promoter sequence for glow-analysis of gene regulation using the naturally secreting Gaussia luciferase reporter.

Features of the pMCS-Gaussia-Dura Luc vector:

• Naturally-secreting Gaussia-Dura luciferase gene, optimized for high expression and glow-stability in mammalian systems
• Multiple cloning site (MCS) provides versatility for transfer of regulatory elements from one plasmid to another
• Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
• Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
• High-copy pUC bacterial DNA replication origin

The Gaussia Dura Luc Vectors contain a mutant form of the Gaussia luciferase gene that confers better bioluminescent signal stability than native luciferase. Gaussia-Dura luciferase (approx. 20kDa) is a secreted protein, which enables measurement of the reporter activity in media (for real-time assays) and in cell lysates. The pMCS vector contains a multiple cloning site for cloning a promoter to study its regulatory potential.

These vectors are subject to a limited use label license.

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