Invitrogen™
Platinum™ Taq DNA Polymerase, DNA-free
Invitrogen Platinum Taq DNA Polymerase, DNA-free, is manufactured using a novel, closed single-use system together with stringent quality-control testing to minimize the risk of contamination with bacterial and human DNA.
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| Catalog Number | No. of Reactions |
|---|---|
| 15966005 | 400 Reactions |
| 15966025 | 2000 Reactions |
Catalog number 15966005
Price (USD)/ 500 U
391.65
Online exclusive
403.00 Save 11.35 (3%)
-
No. of Reactions:
400 Reactions
Price (USD)/ 500 U
391.65
Online exclusive
403.00 Save 11.35 (3%)
Platinum™ Taq DNA Polymerase, DNA-free
Catalog number15966005
Price (USD)/ 500 U
391.65
Online exclusive
403.00 Save 11.35 (3%)
-
Invitrogen Platinum Taq DNA Polymerase, DNA-free, is manufactured using a novel, closed single-use system together with stringent quality-control testing to minimize the risk of contamination with bacterial and human DNA. This enzyme is an ideal choice for PCR-based applications requiring the high sensitivity without false-positive results from reagent-borne contamination. This DNA-free enzyme provides the same high level of performance and lot-to-lot consistency as standard Platinum Taq DNA Polymerase.
Platinum Taq DNA Polymerase, DNA-free, is a recombinant Taq polymerase complexed with a proprietary antibody that blocks polymerase activity at ambient temperatures and dissociates after the initial denaturation step at 94°C. This automatic hot start provides increased sensitivity, specificity, and yield, while allowing convenient reaction assembly at room temperature. Just as with Taq DNA Polymerase, Platinum Taq DNA Polymerase, DNA-free, has a non-template–dependent terminal transferase activity that adds a 3' deoxyadenosine to product ends and has a 5' to 3' exonuclease activity.
Features
- Certified low DNA contamination level (≤0.01 copy of bacterial gDNA per enzyme unit)
- Confidence in qualitative and quantitative detection of low-abundance microorganisms due to signal absence with no-template/reagents-only PCR controls
- Same performance as Platinum Taq DNA Polymerase manufactured using conventional process
Applications
- Amplification of DNA from complex genomic and viral templates
- Hot start PCR
- RT-PCR
For Research Use Only. Not for use in diagnostic procedures.
Specifications
FormatTube
GC-Rich PCR PerformanceLow
PolymerasePlatinum Taq DNA Polymerase
Reaction SpeedStandard
Product TypeTaq DNA Polymerase
Quantity500 Units
Shipping ConditionDry Ice
For Use With (Application)Hot-start PCR
Concentration5 U/μL
Fidelity (vs. Taq)1X
Hot StartBuilt-In Hot Start
No. of Reactions400 Reactions
Overhang3'-A
Reaction FormatSeparate Components
Size (Final Product)5 kb or less
Starting MaterialDNA
Unit Size500 U
Contents & Storage
• Platinum Taq DNA Polymerase, DNA-free, 100 μL
• 10X PCR Buffer (without magnesium), DNA-free, 1.25 mL
• 50 mM MgCl2, DNA-free (1 mL)
Store at -20°C in a non-frost-free freezer.
• 10X PCR Buffer (without magnesium), DNA-free, 1.25 mL
• 50 mM MgCl2, DNA-free (1 mL)
Store at -20°C in a non-frost-free freezer.