Invitrogen™
T4 DNA Ligase (1 U/μL)
T4 DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of ATP between double-stranded DNAs with 3´ hydroxyl Read more
Have Questions?
Change view
| Catalog Number | Quantity |
|---|---|
| 15224017 | 100 U |
| 15224025 | 500 U |
| 15224090 | 4 x 500 U |
Catalog number 15224017
Price (USD)/ 100 units
88.40
Special Offer
Online exclusive
Ends: 16-Dec-2024
104.00 Save 15.60 (15%)
-
Quantity:
100 U
Price (USD)/ 100 units
88.40
Special Offer
Online exclusive
Ends: 16-Dec-2024
104.00 Save 15.60 (15%)
T4 DNA Ligase (1 U/μL)
Catalog number15224017
Price (USD)/ 100 units
88.40
Special Offer
Online exclusive
Ends: 16-Dec-2024
104.00 Save 15.60 (15%)
-
T4 DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of ATP between double-stranded DNAs with 3´ hydroxyl and 5´ phosphate termini. The unique T4 DNA Ligase buffer optimizes ligation, which can be performed in 5 minutes. Single-stranded nucleic acids are not substrates for this enzyme.
Applications
Cloning (blunt-end or cohesive-end ligation) and adding linkers or adapters to blunt-ended DNA
Source
Purified from E. coli lambda lysogen NM989
Performance and quality testing
Endodeoxyribonuclease, 3´ and 5´ exodeoxyribonuclease assays; ligation efficiency tested
Unit definition
One unit catalyzes the exchange of 1 nmol 32P-labeled pyrophosphate into ATP in 20 min at 37°C. One unit is equal to approximately 300 cohesive-end ligation units.
Unit reaction conditions
66 mM Tris-HCl (pH 7.6), 6.6 mM MgCl2, 10 mM DTT, 66 μM ATP, 3.3 μM 32P-labeled pyrophosphate, and enzyme in 0.1 mL for 20 min at 37°C.
Applications
Cloning (blunt-end or cohesive-end ligation) and adding linkers or adapters to blunt-ended DNA
Source
Purified from E. coli lambda lysogen NM989
Performance and quality testing
Endodeoxyribonuclease, 3´ and 5´ exodeoxyribonuclease assays; ligation efficiency tested
Unit definition
One unit catalyzes the exchange of 1 nmol 32P-labeled pyrophosphate into ATP in 20 min at 37°C. One unit is equal to approximately 300 cohesive-end ligation units.
Unit reaction conditions
66 mM Tris-HCl (pH 7.6), 6.6 mM MgCl2, 10 mM DTT, 66 μM ATP, 3.3 μM 32P-labeled pyrophosphate, and enzyme in 0.1 mL for 20 min at 37°C.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration1 U/μL
Shipping ConditionDry Ice
EnzymeLigase
Compatible BufferDNA Ligase Buffer, 5X Reaction Buffer
Quantity100 U
Product TypeT4 DNA Ligase
Unit Size100 units
Contents & Storage
T4 DNA Ligase is supplied with a vial of 5X reaction buffer [250 mM Tris-HCl (pH 7.6), 50 mM MgCl2, 5 mM ATP, 5 mM DTT, 25% (w/v) polyethylene glycol-8000]. Store at -20°C.