Invitrogen™
Platinum™ PCR SuperMix High Fidelity
Platinum PCR SuperMix, High Fidelity, is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification.
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| Catalog Number | No. of Reactions |
|---|---|
| 12532016 | 100 Reactions |
| 12532024 | 5000 Reactions |
Catalog number 12532016
Price (USD)/ 100 rxns
356.65
Online exclusive
394.00 Save 37.35 (9%)
-
No. of Reactions:
100 Reactions
Price (USD)/ 100 rxns
356.65
Online exclusive
394.00 Save 37.35 (9%)
Platinum™ PCR SuperMix High Fidelity
Catalog number12532016
Price (USD)/ 100 rxns
356.65
Online exclusive
394.00 Save 37.35 (9%)
-
Platinum PCR SuperMix, High Fidelity, is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. Designed for high-specificity, high-fidelity DNA amplification, Platinum PCR SuperMix reduces reaction set-up time and only requires the addition of template and primer.
High fidelity is provided by a mixture of Taq DNA polymerase and the proofreading Pyrococcus species GB-D polymerase. High specificity is achieved by anti-Taq antibodies, allowing for automatic hot-start PCR.
Benefits of Platinum PCR SuperMix, High Fidelity
- Six-times higher fidelity than Taq DNA polymerase
- Amplifies fragments up to 15 kb
- Minimizes PCR optimization
- Room-temperature reaction assembly
Applications
- High-fidelity PCR
- Cloning
- Mutagenesis
Notes
- Platinum PCR SuperMix, High Fidelity, produces products that are mixed blunt and 3-A' ends; however, the majority of ends will have a 3-A' overhangs.
- Hot-start capability increases specificity and yield and allows for room-temperature reaction assembly.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
GC-Rich PCR PerformanceLow
PolymerasePlatinum Taq DNA Polymerase High Fidelity
Reaction SpeedStandard
Shipping ConditionWet or Dry Ice
For Use With (Application)Hot-start PCR, High-fidelity PCR
Concentration1.1X
Fidelity (vs. Taq)6X
Hot StartBuilt-In Hot Start
No. of Reactions100 Reactions
OverhangMixed
Reaction FormatSuperMix or Master Mix
Size (Final Product)15 kb or less
Unit Size100 rxns
Contents & Storage
Platinum PCR SuperMix High Fidelity (4 x 1.125 mL)
Store at -20°C in a non-frost-free freezer.
Store at -20°C in a non-frost-free freezer.