AccuPrime™ Taq DNA Polymerase System
| Catalog Number | No. of Reactions |
|---|---|
| 12339024 | 1000 Reactions |
| 12339016 | 200 Reactions |
Platinum anti-Taq DNA polymerase antibodies inhibit polymerase activity, providing an automatic hot-start, while a thermostable accessory protein enhances specific primer-template hybridization during every cycle of PCR. This combination improves the fidelity of Taq DNA Polymerase by two-fold and is ideal for high-throughput screening and multiplex PCR. AccuPrime Taq DNA Polymerase broadens primer annealing temperatures, giving you optimal performance between 55°C and 65°C.
AccuPrime Taq DNA Polymerase features
- Control mispriming at every cycle for single-band PCR products
- Minimal reaction optimization and primer set redesign
- Reaction assembly at room temperature
Applications
AccuPrime Taq DNA Polymerase can be used for a wide variety of applications, including:
- Multiplex PCR
- TOPO TA Cloning
- Allele-specific amplifications
- High-throughput screening
AccuPrime buffers
The 10X AccuPrime buffers contain thermostable AccuPrime protein, Mg++, and deoxyribonucleotide triphosphates at concentrations sufficient to allow amplification during PCR. Two individual buffer systems (10X AccuPrime PCR Buffer I and II) are provided for amplification of specific types of templates. 10X AccuPrime PCR Buffer I is designed for small genomic DNA amplicons (≤200 bp), cDNA, or plasmids. 10X AccuPrime PCR Buffer II is designed for genomic DNA (200 bp–4 kb).
• 10X AccuPrime PCR Buffer I (2 x 1.25 mL)
• 10X AccuPrime PCR Buffer II (2 x 1.25 mL)
• 50 mM Magnesium Chloride (2 x 1.25 mL)
Store at -20°C.