Invitrogen™
AccuPrime™ GC-Rich DNA Polymerase
AccuPrime GC-Rich DNA Polymerase is designed to provide high-yield, high-specificity amplification of difficult-to-amplify templates, such as those with >65% GC content.
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| Catalog Number | No. of Reactions |
|---|---|
| 12337016 | 200 Reactions |
| 12337024 | 1000 Reactions |
Catalog number 12337016
Price (USD)/ 200 rxns
575.65
Online exclusive
598.00 Save 22.35 (4%)
-
No. of Reactions:
200 Reactions
Price (USD)/ 200 rxns
575.65
Online exclusive
598.00 Save 22.35 (4%)
AccuPrime™ GC-Rich DNA Polymerase
Catalog number12337016
Price (USD)/ 200 rxns
575.65
Online exclusive
598.00 Save 22.35 (4%)
-
AccuPrime GC-Rich DNA Polymerase is designed to provide high-yield, high-specificity amplification of difficult-to-amplify templates, such as those with >65% GC content. The kit offers a choice of buffers for amplifying genomic DNA targets (Buffer A) or non-GC-rich cDNA, plasmid, and lambda-based targets (Buffer B).
AccuPrime GC-Rich DNA Polymerase features
- High yields for targets up to 5 kb in length
- AccuPrime accessory proteins for improved PCR specificity
- Sensitivity down to 5 ng of template DNA
Robust and specific amplifications
The AccuPrime buffers contain thermostable proteins that enhance primer-template hybridization during PCR, increasing the specificity of the reaction. The polymerase itself, from the archaebacterium Pyrolobus fumarius, has a five-fold better processivity than Taq DNA polymerase, and remains active even after 4 hours at 95°C.
Unit definition
One unit of AccuPrime GC-Rich DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of dNTPs into acid-insoluble material in 30 min. at 74°C.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
GC-Rich PCR PerformanceHigh
PolymeraseAccuPrime GC-Rich DNA Polymerase
Reaction SpeedStandard
Shipping ConditionWet or Dry Ice
For Use With (Application)Hot-start PCR
Fidelity (vs. Taq)2X
Hot StartBuilt-In Hot Start
No. of Reactions200 Reactions
OverhangBlunt
Reaction FormatSeparate Components
Size (Final Product)5 kb or less
Volume100 μL
Unit Size200 rxns
Contents & Storage
• AccuPrime GC-Rich DNA Polymerase (1 x 100 μL at 2 U/μL)
• 5X AccuPrime GC-Rich Buffer A (1 x 1 mL)
• 5X AccuPrime GC-Rich Buffer B (1 x 1 mL)
• 50 mM MgSO4 (1 x 1 mL)
Store at –20°C.
• 5X AccuPrime GC-Rich Buffer A (1 x 1 mL)
• 5X AccuPrime GC-Rich Buffer B (1 x 1 mL)
• 50 mM MgSO4 (1 x 1 mL)
Store at –20°C.