Invitrogen

CD197 (CCR7) Monoclonal Antibody (3D12), PE, eBioscience™

CD197 (CCR7) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 12

CD197 (CCR7) Antibody (12-1979-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD45RA APC (Product # 17-0458-42) and Rat IgG2a K Isotype Control PE (Product # 12-4321-80) (left) or Anti-Human CD197 (CCR7) PE (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

Product Details

12-1979-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 20 publications 20 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

3D12

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10670625

Product Specific Information

Description: The 3D12 monoclonal antibody reacts with human CCR7, also known as EBI-1 and CD197. CCR7 is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains and functions as a receptor for 6Ckine/SLC (secondary lymphoid-tissue chemokine), CCL19 and CCL21. CCR7 has been shown to be internalized via clathrin-coated pits and the majority recycled back to the plasma membrane. CCR7 is expressed on T cells and can be used to distinguish populations of naive from central and effector memory T cells. CCR7 has been shown to play a role in migration of memory T cells to inflamed tissue. Expression of CCR7 is also found on DC's. During DC maturation CCR7 expression increases and is thought to be involved in a variety of functions: chemotaxis to the lymph node, cellular architecture, rate of endocytosis, survival and maturation. Expression of CCR7 on the cell surface can be down regulated upon ligand binding.

Applications Reported: This 3D12 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 3D12 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

It is recommended that the staining incubation time be increased to at least 45 minutes at 2-8°C for optimal staining.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CCR7 is a member of the G protein coupled receptor family, which is a subfamily of chemokines. CCR7 was identified to be induced by the Epstein Barr virus (EBV), and is thought to be a mediator of EBV effects on B lymphocytes. CCR7 has been reported to be expressed in blood, bone marrow, lymph node, and intestine. CCR7 is particularly expressed in lymphoid tissues and in activated B and T lymphocytes and has been shown to control the migration of memory T cells to inflamed tissues, as well as stimulate dendritic cell maturation. The chemokine (C-C motif) ligand 19 (CCL19/ECL) has been reported to be a specific ligand of this receptor. ESTs have been isolated from blood, embryo, lymph node, and thymus libraries. Receptors for the C - C chemokine family include CCR 1, CCR 2A, CCR 3, CCR 4, CCR 5 and the Duffy blood group antigen. The C-C receptors are important in the function of T cell chemotaxis and migration of phagocytic cells to sites of inflammation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Enhancement of CAR-T cell activity against cholangiocarcinoma by simultaneous knockdown of six inhibitory membrane proteins.

Cancer communications (London, England)

Qiao Y,Chen J,Wang X,Yan S,Tan J,Xia B,Chen Y,Lin K,Zou F,Liu B,He X,Zhang Y,Zhang X,Zhang H,Wu X,Lu L

Published figure using CD197 (CCR7) monoclonal antibody (Product # 12-1979-42) in Flow Cytometry

Sat Jul 01 00:00:00 EDT 2023

Neuroimmune Consequences of eIF4E Phosphorylation on Chemotherapy-Induced Peripheral Neuropathy.

Frontiers in immunology

Agalave NM,Mody PH,Szabo-Pardi TA,Jeong HS,Burton MD

Published figure using CD197 (CCR7) monoclonal antibody (Product # 12-1979-42) in Flow Cytometry

Mon Sep 20 00:00:00 EDT 2021

Alteration of the Immune Microenvironment in HBsAg and HBeAg Dual-Positive Pregnant Women Presenting a High HBV Viral Load.

Journal of inflammation research

Gao F,Wang H,Li X,Guo F,Yuan Y,Wang X,Zhang Y,Bai G

Published figure using CD197 (CCR7) monoclonal antibody (Product # 12-1979-42) in Flow Cytometry

Thu Apr 28 00:00:00 EDT 2022

Intestinal CD11b⁺ B Cells Ameliorate Colitis by Secreting Immunoglobulin A.

Frontiers in immunology

Fu Y,Wang Z,Yu B,Lin Y,Huang E,Liu R,Zhao C,Lu M,Xu W,Liu H,Liu Y,Wang L,Chu Y

12-1979-42 was used in Flow Cytometry to demonstrate that CD11b+ B cells are essential intestinal suppressive immune cells and the primary source of intestinal IgA, which plays an indispensable role in maintaining intestinal homeostasis.

Fri Jan 07 00:00:00 EST 2022

Application of the chemokine-chemokine receptor axis increases the tumor-targeted migration ability of cytokine-induced killer cells in patients with colorectal cancer.

Oncology letters

Zou Y,Liang J,Li D,Fang J,Wang L,Wang J,Zhang J,Guo Q,Yan X,Tang H

12-1979 was used in Flow cytometry/Cell sorting to demonstrate that modification of the CK-CKR axis using exogenous recombinant chemokines at the proper time point enhanced CIK cell trafficking ability and improved CIK antitumor effects.

Wed Jul 01 00:00:00 EDT 2020

Application of the chemokine-chemokine receptor axis increases the tumor-targeted migration ability of cytokine-induced killer cells in patients with colorectal cancer.

Oncology letters

Zou Y,Liang J,Li D,Fang J,Wang L,Wang J,Zhang J,Guo Q,Yan X,Tang H

Wed Jul 01 00:00:00 EDT 2020

The TCR Repertoire Reconstitution in Multiple Sclerosis: Comparing One-Shot and Continuous Immunosuppressive Therapies.

Frontiers in immunology

Amoriello R,Greiff V,Aldinucci A,Bonechi E,Carnasciali A,Peruzzi B,Repice AM,Mariottini A,Saccardi R,Mazzanti B,Massacesi L,Ballerini C

12-1979-42 was used in Flow Cytometry to suggest that the majority of public TCRs belonged to virus-associated sequences.

Fri Mar 26 00:00:00 EDT 2021

Development of CAR-T cell therapy for B-ALL using a point-of-care approach.

Oncoimmunology

de Macedo Abdo L,Barros LRC,Saldanha Viegas M,Vieira Codeço Marques L,de Sousa Ferreira P,Chicaybam L,Bonamino MH

12-1979 was used in Flow cytometry/Cell sorting to demonstrate that the POC approach is a viable alternative for the generation and use of CAR-T cells, overcoming the limitations of current manufacturing protocols.

Wed Jul 14 00:00:00 EDT 2021

Development of CAR-T cell therapy for B-ALL using a point-of-care approach.

Oncoimmunology

de Macedo Abdo L,Barros LRC,Saldanha Viegas M,Vieira Codeço Marques L,de Sousa Ferreira P,Chicaybam L,Bonamino MH

Wed Jul 14 00:00:00 EDT 2021

Single-Cell Profiling Defines Transcriptomic Signatures Specific to Tumor-Reactive versus Virus-Responsive CD4⁺ T Cells.

Cell reports

Magen A,Nie J,Ciucci T,Tamoutounour S,Zhao Y,Mehta M,Tran B,McGavern DB,Hannenhalli S,Bosselut R

Published figure using CD197 (CCR7) monoclonal antibody (Product # 12-1979-42) in Flow Cytometry

Tue Dec 03 00:00:00 EST 2019

Characteristic patterns of HLA presentation and T cell differentiation in adult-onset Still's disease.

International journal of immunopathology and pharmacology

Jung JY,Choi B,Sayeed HM,Suh CH,Kim YW,Kim HA,Sohn S

12-1979 was used in Flow cytometry/Cell sorting to examine the expression of human leukocyte antigen and composition of differentiated T cells in the peripheral blood in patients with adult-onset Still's disease.

Tue Dec 18 00:00:00 EST 2018

Biochanin a Enhances the Defense Against Salmonella enterica Infection Through AMPK/ULK1/mTOR-Mediated Autophagy and Extracellular Traps and Reversing SPI-1-Dependent Macrophage (MΦ) M2 Polarization.

Frontiers in cellular and infection microbiology

Zhao X,Tang X,Guo N,An Y,Chen X,Shi C,Wang C,Li Y,Li S,Xu H,Liu M,Wang Y,Yu L

Published figure using CD197 (CCR7) monoclonal antibody (Product # 12-1979-42) in Flow Cytometry

Fri Aug 30 00:00:00 EDT 2019

Selective Expression of CCR10 and CXCR3 by Circulating Human Herpes Simplex Virus-Specific CD8 T Cells.

Journal of virology

Hensel MT,Peng T,Cheng A,De Rosa SC,Wald A,Laing KJ,Jing L,Dong L,Magaret AS,Koelle DM

12-1979 was used in Flow cytometry/Cell sorting to show that CCL27 drives baseline recruitment of HSV-specific CD8 T cells expressing CCR10.

Sun Oct 01 00:00:00 EDT 2017

Oral Supplementation with Baker's Yeast Beta Glucan Is Associated with Altered Monocytes, T Cells and Cytokines following a Bout of Strenuous Exercise.

Frontiers in physiology

McFarlin BK,Venable AS,Carpenter KC,Henning AL,Ogenstad S

Published figure using CD197 (CCR7) monoclonal antibody (Product # 12-1979-42) in Flow Cytometry

Wed Sep 30 00:00:00 EDT 2020

Single-cell tracking of flavivirus RNA uncovers species-specific interactions with the immune system dictating disease outcome.

Nature communications

Douam F,Hrebikova G,Albrecht YE,Sellau J,Sharon Y,Ding Q,Ploss A

12-1979 was used in Flow cytometry/Cell sorting to demonstrate the tracking of and sense viral RNA at single-cell resolution within complex subsets of the human and murine immune system in different mouse models.

Tue Mar 14 00:00:00 EDT 2017

Resolving TYK2 locus genotype-to-phenotype differences in autoimmunity.

Science translational medicine

Dendrou CA,Cortes A,Shipman L,Evans HG,Attfield KE,Jostins L,Barber T,Kaur G,Kuttikkatte SB,Leach OA,Desel C,Faergeman SL,Cheeseman J,Neville MJ,Sawcer S,Compston A,Johnson AR,Everett C,Bell JI,Karpe F,Ultsch M,Eigenbrot C,McVean G,Fugger L

12-1979 was used in Flow cytometry/Cell sorting to reveal a protective homozygous effect that defined a signalling optimum between autoimmunity and immunodeficiency and identified TYK2 as a potential drug target for autoimmune disorders.

Wed Nov 02 00:00:00 EDT 2016

Durable complete responses off all treatment in patients with metastatic malignant melanoma after sequential immunotherapy followed by a finite course of BRAF inhibitor therapy.

Cancer biology & therapy

Wyluda EJ,Cheng J,Schell TD,Haley JS,Mallon C,Neves RI,Robertson G,Sivik J,Mackley H,Talamo G,Drabick JJ

12-1979 was used in Flow cytometry/Cell sorting to study the effectiveness of different therapy regimes on treating melanoma after failure of immunotherapy.

Tue Mar 01 00:00:00 EST 2016

The APPEESFRS Peptide, Restricted by the HLA-B*35:01 Molecule, and the APPEESFRF Variant Derived from an Autologous HIV-1 Strain Induces Polyfunctional Responses in CD8+ T Cells.

BioResearch open access

Acevedo-Sáenz L,Carmona-Pérez L,Velilla-Hernández PA,Delgado JC,Rugeles L MT

12-1979 was used in Flow cytometry/Cell sorting to determine the effector profile and maturation phenotype of CD8+ T-cells targeting the consensus APPEESFRS (AS9) epitope and its variant APPEESFRF (AF9).

Fri Aug 28 00:00:00 EDT 2015

Calcineurin inhibitor treatment of intravenous immunoglobulin-resistant Kawasaki disease.

The Journal of pediatrics

Tremoulet AH,Pancoast P,Franco A,Bujold M,Shimizu C,Onouchi Y,Tamamoto A,Erdem G,Dodd D,Burns JC

12-1979 was used in Flow cytometry/Cell sorting to investigate treatment of refractory KD with a calcineurin inhibitor.

Sat Sep 01 00:00:00 EDT 2012

Human CD8⁺ and CD4⁺ T cell memory to lymphocytic choriomeningitis virus infection.

Journal of virology

Kotturi MF,Swann JA,Peters B,Arlehamn CL,Sidney J,Kolla RV,James EA,Akondy RS,Ahmed R,Kwok WW,Buchmeier MJ,Sette A

12-1979 was used in Flow cytometry/Cell sorting to analyse the T cell response to acute lymphocytic choriomeningitis virus infection.

Tue Nov 01 00:00:00 EDT 2011

Memory T-cells and characterization of peripheral T-cell clones in acute Kawasaki disease.

Autoimmunity

Franco A,Shimizu C,Tremoulet AH,Burns JC

12-1979 was used in Flow cytometry/Cell sorting to provide insight into the nature and kinetics of the adaptive immune response in Kawasaki disease.

Tue Jun 01 00:00:00 EDT 2010

Role of TLX1 in T-cell acute lymphoblastic leukaemia pathogenesis.

British journal of haematology

Riz I,Hawley TS,Johnston H,Hawley RG

Published figure using CD197 (CCR7) monoclonal antibody (Product # 12-1979-42) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: BLR2; Bukitt's lymphoma receptor 2; C-C chemokine receptor type 7; C-C CKR-7; CC chemokine receptor 7; CD197; CDw197; chemokine (C-C motif) receptor 7; EBI1; EBV-induced G protein-coupled receptor 1; Epstein-Barr virus induced gene 1; Epstein-Barr virus-induced G-protein coupled receptor 1; lymphocyte-specific G protein-coupled peptide receptor; MIP-3 beta receptor

View more View less

Gene Aliases: BLR2; CC-CKR-7; CCR-7; CCR7; CD197; CDw197; CMKBR7; EBI1; EVI1

View more View less

UniProt ID: (Human) P32248

View more View less

Entrez Gene ID: (Human) 1236

View more View less

Function(s)

G-protein coupled receptor activity C-C chemokine receptor activity chemokine (C-C motif) ligand 19 binding chemokine (C-C motif) ligand 21 binding C-C motif chemokine 19 receptor activity C-C motif chemokine 21 receptor activity

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD197 (CCR7) Monoclonal Antibody (3D12), PE, eBioscience™

CD197 (CCR7) Antibody (12-1979-42) in Flow

Product Details

12-1979-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!