Invitrogen

CD127 Monoclonal Antibody (eBioRDR5), PE, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

14 Published Figures

24 References

View all (118) CD127 antibodies

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FIGURE: 1 / 16

CD127 Antibody (12-1278-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD3 APC (Product # 17-0038-42) and Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or Anti-Human CD127 PE (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

CD127 Antibody — Figure 4. miR-21 Promotes Effector Cell Differentiation through AP-1 Activation (A) Naive CD4 + T cells were transfected with either scrambled control or miR-21-blocking locked nucleic acid (LNA21). After 48 hr, PDCD4 and b-actin expression were assessed by western blot. Representative blots and mean normalized intensities from four experiments are shown (mean +- SEM, two-tailed paired t test). (B) Naive CD4 + T cells were activated with anti-CD3 and anti-CD28 beads and transduced with a lentiviral vector expressing scrambled control or anti-miR-21. The representative histogram shows phosphorylated JNK in GFP + cells on day 3. The filled gray histogram represents unstimulated naive CD4 + T cells. Results from 7 experiments are expressed relative to the geometric mean fluorescence intensity (MFI) of controls (mean +- SEM, two-tailed paired t test). (C) Naive CD4 + T cells were activated and transduced as described in (B) and co-transfected with the AP-1 luciferase reporter plasmid and the Renilla luciferase control construct. On day 3, the activity of AP-1 firefly luciferase was measured and normalized to that of Renilla luciferase (n = 4, mean +- SEM, two-tailed paired t test). (D-F) Naive CD4 + T cells isolated from 20- to 35-year-old and 65- to 85-year-old individuals were activated with beads coated with anti-CD3 and anti-CD28 antibodies. (D) PDCD4 expression was quantified by RT-PCR on day 3 and day 5. Results are normalized to ACTB and presented relative to those of cell {TM}

Product Details

12-1278-42

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (0.06 µg)/test

View 23 publications 23 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioRDR5

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10717663

Product Specific Information

Description: The eBioRDR5 monoclonal antibody reacts with human CD127 (Interleukin-7 Receptor alpha). CD127 complexes with CD132, also known as the common gamma chain (gamma c), to form the multi-functional IL-7 receptor (IL-7R). CD127 is a type I glycoprotein with a molecular weight of 75-80 kDa and is expressed by immature B cells through the early pre-B stage, by thymocytes during several stages of their development, and on most mature T cells, with transient down-regulation upon activation. Binding of IL-7 results in signal transduction which occurs through several tyrosine kinase pathways including the Jak/STAT pathway. IL-7 is indispensible for the development of lymphocytes, and the control of homeostatic proliferation of T-cells in the periphery. In addition, IL-7R signaling is know to be involved in the regulation of T cell receptor (TCR) locus rearrangement in gamma delta T cells.

Interestingly, recently it has been demonstrated that CD127 expression is down-regulated on CD4+CD25+ regulatory T cells (T regs). While the co-expression of CD4 and CD25 has become widely used as an indicator of T regs, this method of identification may also include cells without suppressive activity. It has clearly been shown that CD4+CD25+ cells that have down-regulated the expression of CD127 are significantly more highly-enriched for the regulatory T population, as defined by expression of the T reg-specific transcription factor Foxp3 and the suppressive activity of these cells, in vitro.

Binding of the eBioRDR5 monoclonal antibody to PBMCs is blocked by pre-incubation of the cells with recombinant human IL-7 (Product # 14-1079-80).

Applications Reported: This eBioRDR5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioRDR5 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD127 (Interleukin-7, IL-7) is a glycoprotein involved in the regulation of lymphopoiesis. The response of cells to CD127 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is an alpha/gamma chain heterodimer. CD127 consists of an alpha chain and a gamma chain. The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules. CD127 promotes the proliferation of precursor B cells, thymocytes, T cell progenitors, and mature CD4+ and CD8+ T cells. The biological effects of IL7 are mediated by the binding of IL7 to the specific cell surface receptor. Diseases associated with CD127 dysfunction include severe combined immunodeficiency and T cell negative/B cell negative/NK positive severe combined immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Epinephrine Production in Th17 Cells and Experimental Autoimmune Encephalitis.

Frontiers in immunology

Yang P,Tian H,Zou YR,Chambon P,Ichinose H,Honig G,Diamond B,Kim SJ

Published figure using CD127 monoclonal antibody (Product # 12-1278-42) in Flow Cytometry

Mon Jul 05 00:00:00 EDT 2021

CAR T cells targeting tumor-associated exons of glypican 2 regress neuroblastoma in mice.

Cell reports. Medicine

Li N,Torres MB,Spetz MR,Wang R,Peng L,Tian M,Dower CM,Nguyen R,Sun M,Tai CH,de Val N,Cachau R,Wu X,Hewitt SM,Kaplan RN,Khan J,St Croix B,Thiele CJ,Ho M

12-1278-42 was used in Flow cytometry/Cell sorting to investigate if RNA-seq data can be exploited to help identify tumor-associated exons that can be targeted by CAR T cell therapies.

Tue Jun 15 00:00:00 EDT 2021

CD62L Is a Functional and Phenotypic Marker for Circulating Innate Lymphoid Cell Precursors.

Journal of immunology (Baltimore, Md. : 1950)

Bar-Ephraim YE,Koning JJ,Burniol Ruiz E,Konijn T,Mourits VP,Lakeman KA,Boon L,Bögels M,van Maanen JP,Den Haan JMM,van Egmond M,Bouma G,Reijmers RM,Mebius RE

12-1278-42 was used in Flow Cytometry to demonstrate that CD62L not only serves as a functional marker of ILCP, but has potential to be used in the clinic as a diagnostic marker in inflammatory disorders.

Tue Jan 01 00:00:00 EST 2019

A human anti-IL-2 antibody that potentiates regulatory T cells by a structure-based mechanism.

Nature medicine

Trotta E,Bessette PH,Silveria SL,Ely LK,Jude KM,Le DT,Holst CR,Coyle A,Potempa M,Lanier LL,Garcia KC,Crellin NK,Rondon IJ,Bluestone JA

12-1278 was used in Flow cytometry/Cell sorting to suggest that IL-2-F5111.2 may provide an immunotherapy to treat autoimmune diseases and graft-versus-host disease.

Sun Jul 01 00:00:00 EDT 2018

A probiotic modulates the microbiome and immunity in multiple sclerosis.

Annals of neurology

Tankou SK,Regev K,Healy BC,Tjon E,Laghi L,Cox LM,Kivisäkk P,Pierre IV,Hrishikesh L,Gandhi R,Cook S,Glanz B,Stankiewicz J,Weiner HL

Published figure using CD127 monoclonal antibody (Product # 12-1278-42) in Flow Cytometry

Fri Jun 01 00:00:00 EDT 2018

Characterization of CD127^- CD25⁺⁺ Treg from human colostrum.

American journal of reproductive immunology (New York, N.Y. : 1989)

Cérbulo-Vázquez A,Hernández-Peláez G,Arriaga-Pizano LA,Bautista-Pérez P,Romero-Venado J,Flores-González JC,Figueroa-Damian R,Soriano-Becerril D,Mancilla-Herrera I

Published figure using CD127 monoclonal antibody (Product # 12-1278-42) in Flow Cytometry

Thu Feb 01 00:00:00 EST 2018

The Effects of High Mobility Group Box-1 Protein on Peripheral Treg/Th17 Balance in Patients with Atherosclerosis.

Acta Cardiologica Sinica

Ding JW,Zhou T,Zheng XX,Wang XA,Tong XH,Luo CY,Zhang ZQ,Yu B

Published figure using CD127 monoclonal antibody (Product # 12-1278-42) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2018

Activation of miR-21-Regulated Pathways in Immune Aging Selects against Signatures Characteristic of Memory T Cells.

Cell reports

Kim C,Hu B,Jadhav RR,Jin J,Zhang H,Cavanagh MM,Akondy RS,Ahmed R,Weyand CM,Goronzy JJ

Published figure using CD127 monoclonal antibody (Product # 12-1278-42) in Flow Cytometry

Tue Nov 20 00:00:00 EST 2018

Staphylococcus aureus PSM Peptides Modulate Human Monocyte-Derived Dendritic Cells to Prime Regulatory T Cells.

Frontiers in immunology

Richardson JR,Armbruster NS,Günter M,Henes J,Autenrieth SE

12-1278 was used in Flow cytometry/Cell sorting to study the effect of PSMα3 on the maturation, cytokine production, antigen uptake, and T cell stimulatory capacity of human monocyte-derived DCs treated simultaneously with either LPS(TLR4 ligand) or Sa cell lysate (TLR2 ligand).

Thu Oct 03 00:00:00 EDT 2019

Blocking the recruitment of naive CD4⁺ T cells reverses immunosuppression in breast cancer.

Cell research

Su S,Liao J,Liu J,Huang D,He C,Chen F,Yang L,Wu W,Chen J,Lin L,Zeng Y,Ouyang N,Cui X,Yao H,Su F,Huang JD,Lieberman J,Liu Q,Song E

Published figure using CD127 monoclonal antibody (Product # 12-1278-42) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

HDAC inhibition potentiates immunotherapy in triple negative breast cancer.

Oncotarget

Terranova-Barberio M,Thomas S,Ali N,Pawlowska N,Park J,Krings G,Rosenblum MD,Budillon A,Munster PN

Published figure using CD127 monoclonal antibody (Product # 12-1278-42) in Flow Cytometry

Tue Dec 26 00:00:00 EST 2017

Serial immunomonitoring of cancer patients receiving combined antagonistic anti-CD40 and chemotherapy reveals consistent and cyclical modulation of T cell and dendritic cell parameters.

BMC cancer

McDonnell AM,Cook A,Robinson BWS,Lake RA,Nowak AK

Published figure using CD127 monoclonal antibody (Product # 12-1278-42) in Flow Cytometry

Thu Jun 15 00:00:00 EDT 2017

Group 2 innate lymphoid cells are elevated and activated in chronic rhinosinusitis with nasal polyps.

Immunity, inflammation and disease

Poposki JA,Klingler AI,Tan BK,Soroosh P,Banie H,Lewis G,Hulse KE,Stevens WW,Peters AT,Grammer LC,Schleimer RP,Welch KC,Smith SS,Conley DB,Raviv JR,Karras JG,Akbari O,Kern RC,Kato A

Fri Sep 01 00:00:00 EDT 2017

The Distribution of Human Stem Cell-like Memory T Cell in Lung Cancer.

Journal of immunotherapy (Hagerstown, Md. : 1997)

Hong H,Gu Y,Sheng SY,Lu CG,Zou JY,Wu CY

Published figure using CD127 monoclonal antibody (Product # 12-1278-42) in Flow Cytometry

Thu Jan 04 00:00:00 EST 2018

A Systematic Study of the Effect of Different Molecular Weights of Hyaluronic Acid on Mesenchymal Stromal Cell-Mediated Immunomodulation.

PloS one

Gómez-Aristizábal A,Kim KP,Viswanathan S

12-1278 was used in Flow cytometry/Cell sorting to investigate the combined mechanism of action of MSCs and hyaluronic acid.

Wed Jul 06 00:00:00 EDT 2016

DCLRE1C (ARTEMIS) mutations causing phenotypes ranging from atypical severe combined immunodeficiency to mere antibody deficiency.

Human molecular genetics

Volk T,Pannicke U,Reisli I,Bulashevska A,Ritter J,Björkman A,Schäffer AA,Fliegauf M,Sayar EH,Salzer U,Fisch P,Pfeifer D,Di Virgilio M,Cao H,Yang F,Zimmermann K,Keles S,Caliskaner Z,Güner SÜ,Schindler D,Hammarström L,Rizzi M,Hummel M,Pan-Hammarström Q,Schwarz K,Grimbacher B

12-1278 was used in Flow cytometry/Cell sorting to investigate the range of phenotypes associated with DCLRE1C (ARTEMIS) mutations in humans.

Sun Dec 20 00:00:00 EST 2015

Enhanced suppressor function of TIM-3+ FoxP3+ regulatory T cells.

European journal of immunology

Gautron AS,Dominguez-Villar M,de Marcken M,Hafler DA

Published figure using CD127 monoclonal antibody (Product # 12-1278-42) in Flow Cytometry

Mon Sep 01 00:00:00 EDT 2014

Skewed distribution of IL-7 receptor-α-expressing effector memory CD8+ T cells with distinct functional characteristics in oral squamous cell carcinoma.

PloS one

Lee JJ,Yeh CY,Jung CJ,Chen CW,Du MK,Yu HM,Yang CJ,Lin HY,Sun A,Ko JY,Cheng SJ,Chang YL,Chia JS

12-1278 was used in Flow cytometry/Cell sorting to demonstrate that IL-7 receptor expression on CD8+ T cells may differentiate the CD45RA+/2 CCR72 effector memory CD8+ T-cell subsets that exhibit distinct functional characteristics and association with tumour progression in OSCC patients.

Tue Nov 11 00:00:00 EST 2014

Human MAIT cells are xenobiotic-resistant, tissue-targeted, CD161hi IL-17-secreting T cells.

Blood

Dusseaux M,Martin E,Serriari N,Péguillet I,Premel V,Louis D,Milder M,Le Bourhis L,Soudais C,Treiner E,Lantz O

12-1278 was used in Flow cytometry/Cell sorting to investigate the function of mucosal-associated invariant T cells.

Thu Jan 27 00:00:00 EST 2011

Human CD8⁺ and CD4⁺ T cell memory to lymphocytic choriomeningitis virus infection.

Journal of virology

Kotturi MF,Swann JA,Peters B,Arlehamn CL,Sidney J,Kolla RV,James EA,Akondy RS,Ahmed R,Kwok WW,Buchmeier MJ,Sette A

12-1278 was used in Flow cytometry/Cell sorting to analyse the T cell response to acute lymphocytic choriomeningitis virus infection.

Tue Nov 01 00:00:00 EDT 2011

Fc receptor-like 3 protein expressed on IL-2 nonresponsive subset of human regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Nagata S,Ise T,Pastan I

Published figure using CD127 monoclonal antibody (Product # 12-1278-42) in Flow Cytometry

Mon Jun 15 00:00:00 EDT 2009

Phenotypical characterization of human Th17 cells unambiguously identified by surface IL-17A expression.

Journal of immunology (Baltimore, Md. : 1950)

Brucklacher-Waldert V,Steinbach K,Lioznov M,Kolster M,Hölscher C,Tolosa E

12-1278 was used in Flow cytometry/Cell sorting to demonstrate a potential method for using IL-17A as a specific marker for human and mouse Th17 cells.

Sun Nov 01 00:00:00 EDT 2009

Loss of IL-7 receptor alpha on CD4+ T cells defines terminally differentiated B cell-helping effector T cells in a B cell-rich lymphoid tissue.

Journal of immunology (Baltimore, Md. : 1950)

Lim HW,Kim CH

Sat Dec 01 00:00:00 EST 2007

Loss of IL-7 receptor alpha on CD4+ T cells defines terminally differentiated B cell-helping effector T cells in a B cell-rich lymphoid tissue.

Journal of immunology (Baltimore, Md. : 1950)

Lim HW,Kim CH

Sat Dec 01 00:00:00 EST 2007

Bioinformatics

Protein Aliases: CD127; CD127 antigen; CDw127; IL 7 receptor; IL 7R a; IL 7R subunit alpha; IL 7R α; IL 7Ra; IL 7Rα; IL-7 receptor subunit alpha; IL-7R; IL-7R subunit alpha; IL7 receptor; IL7 receptor subunit alpha; IL7R a; IL7R alpha; IL7R subunit alpha; IL7R α; IL7Rα; interleukin 7 receptor alpha chain; interleukin 7 receptor isoform H5-6; Interleukin-7 receptor subunit alpha; Interleukin7 receptor subunit alpha; MGC107557; sCD127; soluble IL 7 receptor; soluble IL 7R

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Gene Aliases: CD127; CDW127; IL-7R-alpha; IL7R; IL7RA; ILRA

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UniProt ID: (Human) P16871

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Entrez Gene ID: (Human) 3575

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Function(s)

antigen binding interleukin-7 receptor activity protein binding transmembrane signal receptor

Process(es)

regulation of DNA recombination cell morphogenesis negative regulation of T cell mediated cytotoxicity immunoglobulin production immune response signal transduction cell surface receptor signaling pathway regulation of cell size positive regulation of gene expression cell growth T cell differentiation positive regulation of T cell differentiation in thymus interleukin-7-mediated signaling pathway B cell proliferation lymph node development homeostasis of number of cells

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CD127 Monoclonal Antibody (eBioRDR5), PE, eBioscience™

CD127 Antibody (12-1278-42) in Flow

Product Details

12-1278-42

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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