Invitrogen

CD11a (LFA-1alpha) Monoclonal Antibody (M17/4), PE, eBioscience™

CD11a (LFA-1alpha) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 3

CD11a (LFA-1alpha) Antibody (12-0111-82) in Flow

Staining of BALB/c splenocytes with 0.25 µg of Rat IgG2a K Isotype Control PE (Product # 12-4321-80) (blue histogram) or 0.25 µg of Anti-Mouse CD11a PE (purple histogram). Cells in the lymphocyte gate were used for analysis.

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Product Details

12-0111-82

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.5 µg/test

View 15 publications 15 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

M17/4

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_465544

Product Specific Information

Description: The M17/4 monoclonal antibody reacts with mouse CD11a, the 180 kDa integrin alpha L, also known as the lymphocyte function associated antigen-1 (LFA-1) alpha chain. LFA-1, formed by non-covalent association of CD11a with CD18 (integrin beta 2), serves as an important adhesion molecule involved in lymphocyte and granulocyte function. CD54 (ICAM-1), CD102 (ICAM-2), and CD50 (ICAM-3) are ligands for LFA-1. CD11a is expressed by all leukocytes.

Applications Reported: The M17/4 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The M17/4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD11a (LFA-1 alpha, ITGAL) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. CD11a plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. CD11a is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of CD11a to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. CD11a is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of CD11a function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti- CD11a antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation, binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity. Two transcript variants encoding different isoforms of CD11a have been found. Diseases associated with CD11a dysfunction include benign cephalic histiocytosis and leukocyte adhesion deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Modulating CD40 and integrin signaling in the proinflammatory nexus using a 15-amino-acid peptide, KGYY₁₅.

The Journal of biological chemistry

Vaitaitis GM,Wagner DH

Published figure using CD11a (LFA-1alpha) monoclonal antibody (Product # 12-0111-82) in Flow Cytometry

Mon May 01 00:00:00 EDT 2023

Modulating CD40 and integrin signaling in the proinflammatory nexus using a 15-amino-acid peptide, KGYY₁₅.

The Journal of biological chemistry

Vaitaitis GM,Wagner DH

Published figure using CD11a (LFA-1alpha) monoclonal antibody (Product # 12-0111-82) in Flow Cytometry

Mon May 01 00:00:00 EDT 2023

Single-cell RNA and protein profiling of immune cells from the mouse brain and its border tissues.

Nature protocols

Scheyltjens I,Van Hove H,De Vlaminck K,Kancheva D,Bastos J,Vara-Pérez M,Pombo Antunes AR,Martens L,Scott CL,Van Ginderachter JA,Saeys Y,Guilliams M,Vandamme N,Movahedi K

12-0111-82 was used in Flow cytometry/Cell sorting to provide a protocol that outlines the steps involved in performing single-cell multi-omic analysis of the brain immune compartment in mice, high-dimensional flow cytometry and droplet-based single-cell RNA sequencing, and the steps involved in analysis and interpretation of data obtained.

Sat Oct 01 00:00:00 EDT 2022

Quorum Regulation via Nested Antagonistic Feedback Circuits Mediated by the Receptors CD28 and CTLA-4 Confers Robustness to T Cell Population Dynamics.

Immunity

Zenke S,Palm MM,Braun J,Gavrilov A,Meiser P,Böttcher JP,Beyersdorf N,Ehl S,Gerard A,Lämmermann T,Schumacher TN,Beltman JB,Rohr JC

12-0111 was used in Flow cytometry/Cell sorting to conclude that CD8+ T cell-population-intrinsic mechanisms regulate cellular behavior, thereby promoting robustness of population dynamics.

Tue Feb 18 00:00:00 EST 2020

The Innate Immune Response to Infection Induces Erythropoietin-Dependent Replenishment of the Dendritic Cell Compartment.

Frontiers in immunology

Einwächter H,Heiseke A,Schlitzer A,Gasteiger G,Adler H,Voehringer D,Manz MG,Ruzsics Z,Dölken L,Koszinowski UH,Sparwasser T,Reindl W,Jordan S

12-0111-82 was used in Flow Cytometry to investigate the molecular and cellular mechanisms of inflammation-induced dendritic cell generation.

Mon Apr 19 00:00:00 EDT 2021

HVEM and CD160: Regulators of Immunopathology During Malaria Blood-Stage.

Frontiers in immunology

Muscate F,Stetter N,Schramm C,Schulze Zur Wiesch J,Bosurgi L,Jacobs T

12-0111 was used in Flow cytometry/Cell sorting to study the requirement of the co-stimulatory molecule HVEM to maintain CD8+ T effector populations during malarial infection.

Thu Oct 03 00:00:00 EDT 2019

A DOCK8-WIP-WASp complex links T cell receptors to the actin cytoskeleton.

The Journal of clinical investigation

Janssen E,Tohme M,Hedayat M,Leick M,Kumari S,Ramesh N,Massaad MJ,Ullas S,Azcutia V,Goodnow CC,Randall KL,Qiao Q,Wu H,Al-Herz W,Cox D,Hartwig J,Irvine DJ,Luscinskas FW,Geha RS

12-0111 was used in Flow cytometry/Cell sorting to show that Wiskott-Adrich syndrome and dedicator of cytokinesis (DOCK8) deficiency have common pathogenic mechanisms, with the DOCK9-WIP-WASp linking T-cell receptors with the actin cytoskeleton.

Mon Oct 03 00:00:00 EDT 2016

Monocyte Adhesion and Plaque Recruitment During Atherosclerosis Development Is Regulated by the Adapter Protein Chat-H/SHEP1.

Arteriosclerosis, thrombosis, and vascular biology

Herbin O,Regelmann AG,Ramkhelawon B,Weinstein EG,Moore KJ,Alexandropoulos K

12-0111 was used in Flow cytometry/Cell sorting to analyse the role of Chat-H in atherosclerosis development, showing that monocyte adhesion and plaque recruitment is regulated by Chat-H/SHEP1.

Thu Sep 01 00:00:00 EDT 2016

Real-time in vivo imaging reveals the ability of neutrophils to remove Cryptococcus neoformans directly from the brain vasculature.

Journal of leukocyte biology

Zhang M,Sun D,Liu G,Wu H,Zhou H,Shi M

12-0111 was used in Flow cytometry/Cell sorting to demonstrate direct removal of Cryptococcus neoformans by neutrophils from its arrested site as a potential host defense mechanism.

Tue Mar 01 00:00:00 EST 2016

PI3Kδ promotes CD4(+) T-cell interactions with antigen-presenting cells by increasing LFA-1 binding to ICAM-1.

Immunology and cell biology

Garçon F,Okkenhaug K

12-0111 was used in Immunocytochemistry-immunofluorescence to investigate the signalling pathways that control integrin affinities.

Sun May 01 00:00:00 EDT 2016

CD137 Agonist Therapy Can Reprogram Regulatory T Cells into Cytotoxic CD4+ T Cells with Antitumor Activity.

Journal of immunology (Baltimore, Md. : 1950)

Akhmetzyanova I,Zelinskyy G,Littwitz-Salomon E,Malyshkina A,Dietze KK,Streeck H,Brandau S,Dittmer U

12-0111 was used in Flow cytometry/Cell sorting to study the mechanisms of immunological tumour control by CD4(+) T cells in the course of CD137 agonist immunotherapy in the absence of a CD8 T cell response.

Fri Jan 01 00:00:00 EST 2016

P2RX7 sensitizes Mac-1/ICAM-1-dependent leukocyte-endothelial adhesion and promotes neurovascular injury during septic encephalopathy.

Cell research

Wang H,Hong LJ,Huang JY,Jiang Q,Tao RR,Tan C,Lu NN,Wang CK,Ahmed MM,Lu YM,Liu ZR,Shi WX,Lai EY,Wilcox CS,Han F

12-0111 was used in Flow cytometry/Cell sorting to demonstrate the role of the P2RX7 pathway in linking neurovascular inflammation to brain damage in vivo.

Mon Jun 01 00:00:00 EDT 2015

Interleukin-7 and anti-programmed cell death 1 antibody have differing effects to reverse sepsis-induced immunosuppression.

Shock (Augusta, Ga.)

Shindo Y,Unsinger J,Burnham CA,Green JM,Hotchkiss RS

Published figure using CD11a (LFA-1alpha) monoclonal antibody (Product # 12-0111-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2015

Self-recognition of the endothelium enables regulatory T-cell trafficking and defines the kinetics of immune regulation.

Nature communications

Fu H,Kishore M,Gittens B,Wang G,Coe D,Komarowska I,Infante E,Ridley AJ,Cooper D,Perretti M,Marelli-Berg FM

12-0111 was used in Flow cytometry/Cell sorting to explain the mechanisms underlying the powerful migratory capacity of Treg cells and their ability to maintain optimal T cell ratios.

Fri Mar 14 00:00:00 EDT 2014

Depletion of alveolar macrophages ameliorates virus-induced disease following a pulmonary coronavirus infection.

PloS one

Hartwig SM,Holman KM,Varga SM

12-0111 was used in Flow cytometry/Cell sorting to demonstrate that alveolar macrophages play a critical role in modulating MHV-1-induced morbidity and mortality.

Tue Dec 30 00:00:00 EST 2014

Centrosome docking at the immunological synapse is controlled by Lck signaling.

The Journal of cell biology

Tsun A,Qureshi I,Stinchcombe JC,Jenkins MR,de la Roche M,Kleczkowska J,Zamoyska R,Griffiths GM

12-0111 was used in Flow cytometry/Cell sorting to investigate the role of Lck signalling in the control of centrosome docking at the immunological synapse.

Mon Feb 21 00:00:00 EST 2011

Synergistic antitumor effects of CpG oligodeoxynucleotide and STAT3 inhibitory agent JSI-124 in a mouse melanoma tumor model.

Immunology and cell biology

Molavi O,Ma Z,Hamdy S,Lai R,Lavasanifar A,Samuel J

12-0111 was used in Flow cytometry/Cell sorting to provide the proof-of-principle for combining CpG and JSI-124 to enhance anti-tumour immune responses.

Mon Oct 20 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: alpha L integrin; antigen CD11A p180; CD11 antigen-like family member A; CD11a; Integrin alpha-L; integrin alphaL; Leukocyte adhesion glycoprotein LFA-1 alpha chain; Leukocyte function-associated molecule 1 alpha chain; LFA-1A; Ly-15; Lymphocyte antigen 15; lymphocyte function associated antigen 1, alpha polypeptide

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Gene Aliases: (p180); Cd11a; Itgal; LFA-1; LFA-1A; Ly-15; Ly-21

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UniProt ID: (Mouse) P24063

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Entrez Gene ID: (Mouse) 16408

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Function(s)

ICAM-3 receptor activity protein complex binding metal ion binding protein heterodimerization activity cell adhesion molecule binding integrin

Process(es)

T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell cell adhesion heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules leukocyte cell-cell adhesion cell-matrix adhesion signal transduction cell surface receptor signaling pathway integrin-mediated signaling pathway single organismal cell-cell adhesion regulation of cell-cell adhesion positive regulation of cell-cell adhesion positive regulation of T cell proliferation receptor clustering activated T cell proliferation positive regulation of calcium-mediated signaling

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD11a (LFA-1alpha) Monoclonal Antibody (M17/4), PE, eBioscience™

CD11a (LFA-1alpha) Antibody (12-0111-82) in Flow

Product Details

12-0111-82

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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