Ham's F-12 Nutrient Mix
| Catalog Number | Quantity |
|---|---|
| 11765070 | 6 x 1000 mL |
| 11765054 | 500 mL |
| 11765062 | 10 x 500 mL |
| 11765047 | 1000 mL |
Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free, single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. We offer a variety of F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This Ham's F-12 is modified as follows:
| With |
| • L-glutamine |
| • Phenol Red |
The complete formulation is available.
Using F-12
Compared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine. F-12 contains no proteins or growth factors. Therefore, F-12 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-12 uses a sodium bicarbonate buffer system (1.176 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture