RNA UltraSense™ One-Step Quantitative RT-PCR System

The RNA UltraSense™ One-Step Quantitative RT-PCR System is specially designed for amplification and real-time quantification of ultra-low abundance transcripts and RNA viruses. The optimized, ultra-concentrated system combines SuperScript™ III RT and Platinum™ Taq DNA Polymerase to provide greater priming specificity, higher product yields, and detection over a broad dynamic range. The high concentration formulation provides great flexibility with low concentration / high volume RNA samples, and improves performance with targets rich in secondary structure. As little as 25 pg/μl total RNA can be detected with a PCR efficiency of 97% (Figure 1). The RNA UltraSense™ System features:

• SuperScript™ III RT for higher temperature cDNA synthesis (up to 60°C) for greater success with RNA secondary structure
• Platinum™ Taq DNA Polymerase with hot-start technology for improved specificity
• Optimized performance with either LUX™ Fluorogenic Primers or dual-labeled fluorogenic probes
• Proprietary, ultra-concentrated buffer for addition of large sample volumes (up to 70%) and success with RNA secondary structure
• Validated performance on multiple real-time instrument platforms
• One-step format for speed, convenience, and less reaction-to-reaction variability

Applications:
Real-time quantitative amplification of RNA, including:
• RNA viruses
• Low-abundance RNA transcripts
• Difficult-to-amplify RNA targets
• High-value RNA detection and research