Dynabeads™ CD15 are superparamagnetic beads covalently coupled with an anti-human CD15 antibody that efficiently depletes human CD15+ myeloid cells (predominantlyRead more
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Catalog number 11137D
Price (USD)/ 5 mL
1,006.65
Online exclusive
1274.00
Save 267.35 (21%)
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Add to cart
Price (USD)/ 5 mL
1,006.65
Online exclusive
1274.00
Save 267.35 (21%)
Add to cart
Dynabeads™ CD15
Catalog number11137D
Price (USD)/ 5 mL
1,006.65
Online exclusive
1274.00
Save 267.35 (21%)
-
Add to cart
Dynabeads™ CD15 are superparamagnetic beads covalently coupled with an anti-human CD15 antibody that efficiently depletes human CD15+ myeloid cells (predominantly neutrophil and eosinophil granulocytes) directly from whole blood, buffy coat, or PBMC samples. Dynabeads™ CD15 can also be used to positively isolate CD15+ cells for molecular applications. Advantages of Dynabeads™ CD15:
• Efficient depletion of granulocytes (neutrophils and eosinophils) • Isolation of granulocytes directly from whole blood for molecular applications • No columns required
About Dynabeads™ CD15 Dynabeads™ CD15 are uniform, superparamagnetic polystyrene beads (4.5 μm diameter) coated with a monoclonal mouse IgM antibody recognizing the human CD15 membrane antigen. The CD15 antigen is predominantly expressed on human neutrophil and eosinophil granulocytes, and to a varying degree on monocytes.
Magnetic bead-;based separation offers easy handling Dynabeads™ CD15 is added to the sample under continuous mixing to optimize the binding of the Dynabeads™ to the target cells. By placing the sample on a magnet, it separates the bead-bound target cells from the rest of the sample in just 1–2 minutes. For depletion, remove the supernatant to a new tube for further studies and discard the bead-bound cells. For positive isolation for molecular studies, remove the supernatant and wash the bead-bound cells 2–3 times in buffer to get optimal purity. The cells can be lysed while still attached to the beads and the supernatant transferred to a new tube for downstream molecular analysis. Starting samples can be whole blood, PBMC, or buffy coat.