DMEM, low glucose, pyruvate, no glutamine, no phenol red
| Catalog Number | Quantity |
|---|---|
| 11054001 | 1 L |
| 11054020 | 500 mL |
| 11880036 also known as 11880-036 | 10 x 500 mL |
Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of Gibco DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This DMEM is modified as follows:
| With | Without |
| • Low Glucose | • L-glutamine |
| • Sodium Pyruvate | • Phenol Red |
| • HEPES |
The complete formulation is available.
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate.
Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.
DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.