POROS Reversed-Phase Media for scale-up of separations developed with POROS 20 micron Self Pack media. POROS R1 is a 4000 angstrom pore size Poly[styrenevinylbenzene] particle, suitable for the reversed-phase separation of biomolecules.
POROS High Performance Chromatography™ Media and Pre-Packed Columns
Reduce time-consuming sample prep
You can replace many sample preparation steps with high speed High Performance Chromatography technology. For instance, dialysis of large volumes of material can be replaced by high flow rate processing of the dilute sample. Elution in a small volume of new buffer accomplishes both buffer exchange and concentration.
Create novel assays for more efficient analysis
High Performance Chromatography technology is not limited to standard modes of separation. Both enzymes and affinity ligands can be immobilized on POROS media. By combining rapid on-column protein digestions with rapid on-column immunoassays and chromatographic separations, you can create entirely new assays with unlimited potential.
High capacity, high resolution, high speed
In contrast to conventional chromatography media, POROS High Performance Chromatography™ resins particles, are engineered to have two discreet classes of pores. Large 'throughpores' allow convection flow to occur through the particles themselves, quickly carrying sample molecules to short 'diffusive' pores inside. By reducing the distance over which diffusion needs to occur, the time required for sample molecules to interact with interior binding sites is also reduced. Diffusion is no longer limiting and flow rates can be dramatically increased–without compromising resolution or capacity. Separations can be achieved at 1000 to 5000 cm/hr compared to 50 to 360 cm/hr for conventional media.