SureLock™ Tandem Midi Welcome Pack, nitrocellulose

The Invitrogen SureLock Tandem Midi Welcome Pack, nitrocellulose, is designed specifically for electrophoresis and wet transfer of Invitrogen pre-cast midi gels using nitrocellulose transfer membranes. The innovative 2-in-1 gel electrophoresis and transfer tank enables electrophoresis and transfer of high-performance Invitrogen midi gels using the same tank.

The SureLock Tandem Midi Welcome Pack, nitrocellulose, contains:
• SureLock Tandem Midi Gel Tank
• Two SureLock Tandem Midi Blot modules
• SureLock Tandem Transfer Tray
• SureLock Tandem Midi Pre-cut Membranes and Filter, 0.2 μm, nitrocellulose

About the SureLock Tandem Midi Gel Tank
The SureLock Tandem Midi Gel Tank provides for rapid electrophoresis of midi gels using minimal buffer (∼520 mL/gel) in a leak-free system. With a set-up time of ∼30 seconds, the tank efficiently runs midi gels while also providing consistent performance. The tank features two independent chambers, allowing electrophoresis of one or two gels at a time, which saves on buffer and limits waste. The tank is compatible with all Invitrogen pre-cast midi gels and midi gel cassettes.

About the SureLock Tandem Midi Blot Module
The SureLock Tandem Midi Blot Module is a wet transfer device used exclusively with the SureLock Tandem Midi Gel Tank. The tank accommodates one blot module per chamber, or two blot modules total. The SureLock Tandem Midi Blot Module is compatible with all Invitrogen midi gels (blot size: 8.5 cm x 14 cm). The blot module accommodates one midi gel per wet transfer. The unique design make the module easy to use, while the efficient size of the inner core uses less transfer buffer per transfer (only ∼300 mL per transfer). Since transfer buffer contains methanol, this also results in less hazardous waste requiring special disposal. At the recommended conditions and constant voltage, proteins can be transferred to nitrocellulose or PVDF membranes in 30 minutes and at room temperature.