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Real-time PCR master mixes for gene expression analysis

Robust gene expression analysis requires consistent, linear amplification of targets across a wide range of concentrations. Because target concentrations vary, it is important to use a qPCR master mix with a wide dynamic range to help ensure you don’t miss detectable changes in expression levels. Applied Biosystems real-time PCR master mixes for gene expression analysis are specifically formulated to efficiently detect targets at both high and very low concentrations, enabling you to capture as much data as possible. View our gene expression master mix product recommendations below.


Which qPCR master mix is right for you?

 


TaqMan Fast Advanced Master Mix


TaqMan Fast Virus 1-Step master mixes


PowerTrack SYBR Green Master Mix*

 

Check price or order now

Check price or order now

Check price or order now

Key application

Gene expression analysis

Gene expression analysis

Gene expression analysis

Chemistry

2-step probe-based

1-step probe-based

2-step SYBR Green-based

Target

cDNA

RNA

cDNA

Concentration

2X

4X

2X

Multiplexing and passive reference dye

With ROX:  up to 2 targets

Available in 2 versions:
With ROX: up to 3 targets

No ROX: 3 or more targets 

With ROX: 1 target

Cycling mode/run time

Fast:  ~40 min

Standard: ~50 min

Fast: ~60 min

Standard: ~90 min

Fast: ~30 min

Standard: ~60 min

Carryover contamination control

Available in 2 versions:

With UNG

No UNG

No UNG

With UNG

Reverse transcriptase

Purchase SuperScript IV VILO Master Mix separately and use before qPCR

Thermostable MMLV RT included in master mix

Purchase SuperScript IV VILO Master Mix separately and use before qPCR

Assay recommendations

TaqMan Gene Expression assays

TaqMan Gene Expression assays

Sequence detection primers

 

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Request a sample

Request a sample

* For SYBR Green detection: when you need up to 72 hours of benchtop stability, choose PowerUp SYBR Green Master Mix in combination with High-Capacity RNA-to-cDNA Kit Reverse Transcriptase.


TaqPath master mixes*

Applied Biosystems TaqPath general purpose reagents (using TaqMan chemistry) are designed for use in assay development, biopharma development, manufacturing, and QC, and other applications requiring reagents manufactured under current good manufacturing practices (cGMP) and compliance documentation.

*For Laboratory Use.

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