Will image-enhanced flow cytometry become commonplace?
We interviewed Hervé Luche, PhD.
Learn more about image-enhanced flow cytometry they discussed.
Immunology at Work is here for you, whether you want to review the basics, gain more in-depth knowledge, or discover the latest research tools. This resource hub contains rich and reliable technical content designed for new and experienced life scientists alike exploring the field of immunology.
How did spectral flow cytometry change your perspective on your work?
We interviewed Cyrille Mionnet, PhD.
Learn more about NovaFluor dyes they discussed.
Biomarker discovery solutions
Biomarker research in immunology offers crucial insights for disease diagnosis, prognosis, treatment response, mechanistic understanding, drug development, and predictive/preventive medicine.
Discover the different immune cells and their role in immunology. For each cell category, you can find information on its development, differentiation, effector function, and role in disease, as well as common methods used for its study and cellular markers that are relevant for phenotyping. Click on the cell type below to learn more.
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Natural Killer cells
NK cells kill pathogen infected cells and cancer cells. They also release cytokines, which alert and attract other immune cells.
T Helper 1 cells
Lineage of CD4+ T helper cells that promote immune response required for host defense against intracellular viral and bacterial pathogens.
T Follicular Helper cells
Subset of CD4+ T helper cells that trigger germinal center B cells into antibody-secreting plasma and memory B cells.
Monocytes
Circulating immune cells that engulfs (phagocytosis) and activates other immune cells.
T Helper 2 cells
Lineage of CD4+ T helper cells that promote humoral immunity critical for host defense against extracellular pathogens.
Cytotoxic T cells
T cells, also know as CD8+ cells, that kill virus infected cells and cancer cells by releasing toxic chemicals.
Macrophages
Professional phagocytic tissue resident cells that destroy foreign antigens/cells by phagocytosis. They release cytokines that attract and activates other immune cells.
Regulatory T cells
T cells critical to the maintenance of self-tolerance and immune cell homeostasis.
Dendritic cells
Phagocytic antigen presenting cells with an important role in alerting T cells to new pathogens. They act as key mediators between the innate and adaptive immune systems.
T Helper 17 cells
CD4+ T helper cells that play a critical role in the activation of other immune cells such as B cells and cytotoxic T cells, as well as in the regulation of immune responses.
B Cells
B cells play a critical role in the adaptive immune response, producing high-affinity antibodies, acting as antigen-presenting cells, and secreting cytokines.
Granulocytes
Granulocytes are a heterogenous category of leukocytes, comprising neutrophils, eosinophils, basophils, and mast cells.
iNKT cells
Invariant natural killer T (iNKT) cells display properties of both T cells and natural killer (NK) cells and help to bridge the innate and adaptive immune responses.
Inflammation
Inflammation is an organism’s protective response to pathogens, infection, or tissue damage and involves the coordinated communication of a variety of immune cells and blood vessels. Understand the general phenomenon of inflammation and triggers to the process.
Cytokine storm
Learn about cell types, cytokines, and markers involved with uncontrolled inflammation.
Pattern recognition receptors
Find information on pattern-recognition receptors (PRRs) including pathogen-associated molecular patterns (PAMPs) and damage-associated molecules patterns (DAMPs).
Viral life cycle
Find information on viral attachment and entry, replication, maturation, and release.
Interferons
Interferon (IFN) proteins are a family of cytokines secreted by host cells to modulate the immune response. Find information on interferon types and induction.
Pro-inflammatory cytokines
Cytokines are small secreted proteins that are key modulators of inflammation. Discover how pro-inflammatory cytokines play a role in the immune response.
Protocols for Immunology
Apply the background information provided throughout this resource center with practical and relevant application protocols. Browse by application or technique:
Cell preparation and immune cell stimulation
Find detailed protocols on the isolation of the different immune cells starting from whole blood or lymphoid tissue and various methods to stimulate them.
Sample preparation
Protocols covering isolation of RNA from immune cells to sample preparation for immunoassays.
Flow cytometry
Detailed protocols on staining, compensation, and validated antibody panels.
Immunoassays
Find general protocols for ELISA, western blot analysis, and immunohistochemistry.
Western blotting
A collection of general western blotting protocols for chemiluminescent and fluorescent detection.
Featured eBooks
Flow Cytometry Protocols Handbook
Protocols in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping and data analysis strategies.
Flow Cytometry Capability Guide
Learn about our comprehensive suite of flow cytometry solutions to deliver you high-performance results and save you time.
Flow Cytometry Reagent Selection Guide
A handy reference poster featuring the broad range of Invitrogen labeling reagents available for flow cytometry.
Biomarker Quantitation Guide
Let us help you navigate the different tools available for protein and RNA quantitation with valuable technical and product information.
Article collection and posters
Flow and Sorting Article Collection
Get the latest in flow cytometry and cell sorting innovation in these collections of articles.
Human CD marker poster
An extensive selection of Invitrogen eBioscience antibody conjugates of human CD markers.
Mouse CD marker poster
View the many options for multicolor flow cytometry research in one place.
Immune cell guide
Discover immune cell markers for humans and mice. CD and non-CD markers, alternate antigen names, distribution, and function.
Video Tutorials for Immunology Research
Find on-demand tutorials and register for upcoming webinars to get firsthand knowledge of the latest technologies to accelerate your immunology research.
Start watching
Featured webinar
Cell-based assays to functionally characterize immunotherapy and the immune response to viral infection.
Research tools for Immunology
Digital tools to make your experimental planning easier.
Flow Cytometry Panel Design Service and Panel Builder Tool
Design your flow cytometry panels effortlessly with our FREE Panel Design Service by our scientists, or use our Panel Builder Tool for a DIY approach.
Fluorescence SpectraViewer
Assess the spectral compatibility of dyes and probes for experiments that utilize fluorescence detection techniques.
ProcartaPlex Panel Configurator
Design your own multiplex immunoassay for protein detection using a Luminex instrument.
Meet the Scientists
Learn about the scientists who helped put this resource center together.
Inspiring scientists making a difference
We asked scientists in immunology research about the challenges they face, how they overcome them and what keeps them motivated. What we learned was so inspiring that we created this space to share their stories with you.
Be the spark story – Martin Pelletier
“I feel blessed that I have a family that I love, that I can do what I love to do. Knowing that some people are restrained, they have disease that doesn't allow them to have a similar life as me, that drives me.”
Be the spark story – Pratip Chattopadhyay
“I was a cancer patient and a cancer survivor 25 years ago. I had these questions about how my disease would go. And that's exactly the work that we do here at Talon.”
Be the spark story – Lisa Ng
“It's not just about publishing in high impact journals, but also ensuring that the outcome has very practical use, to change lives.”
Unraveling different ways of tackling common research challenges
An idea is like a spark in the dark. It makes everything brighter. This blog series uncovers common challenges in immunology research experiments and spark different ways of thinking to discover solutions.
Build with confidence
How can you bring flow cytometry panel design up to speed?
Revealing the big picture
How much data can you get from a single sample in immunoassays?
Recipe for success
Why are protocols hard to find?
Spectral unmixing
How cell sorting is becoming an even more powerful tool?
Seeing is believing
How cell images enable more accuracy in flow cytometry data
Standardization challenges
How to compare your flow cytometry data across experiments?
Pushing the limits
What are ways of expanding flow cytometry panels?
Two applications one instrument
What are the benefits of performing proteomics and genomics assays on the same instrument?
Style Sheet for Global Design System
Style Sheet for Komodo Tabs
For Research Use Only. Not for use in diagnostic procedures.