LAMP Applications for viral pathogen detection
Advance your viral testing and research with Loop-mediated isothermal amplification (LAMP) technology for simple, fast and affordable solutions for viral pathogen research and surveillance. Choose from our RT-LAMP products below to simplify and accelerate detection of RNA targets to optimize your research workflow.
LAMP technology is increasingly used in molecular diagnostics to accelerate the detection of viral, bacterial, and parasitic pathogens.
LAMP Technology
Loop-mediated isothermal amplification (LAMP) is a simple, sensitive, fast and affordable solution for RNA and DNA detection, with several methods for evaluating results. With a simple workflow and fast reaction speed, it is useful for in field applications for faster detection and surveillance of viral pathogens.
SARS-CoV-2: Research and surveillance
Invitrogen Colorimetric ReadiLAMP™ SARS-CoV-2 Kit – assay for SARS-CoV-2 surveillance with simple workflow, and rapid results resulting in robust performance.
Invitrogen SuperScript IV RT-LAMP Master Mix – reagents for faster and simpler detection of RNA targets with maximum flexibility to optimize viral pathogen research and assay development for SARS-CoV-2, measles, influenza and other pathogens.
RT-LAMP Protocol and Application Note – standalone reagents for fast and simple testing and surveillance of viral pathogens, including SARS-CoV-2.
Other infectious diseases and applications
RT-LAMP reagents provide faster and simpler solutions to validate additional research and assay development.
Invitrogen SuperScript IV RT-LAMP Master Mix, and
- Detection of pathogens (viruses, bacteria, fungi)
- Molecular diagnostics research for food and water safety
- Cancer research and diagnostics
RT-LAMP reagents, master mix and assay for research and surveillance of viral pathogens
RT-LAMP or reverse transcription loop-mediated isothermal amplification can simplify and accelerate your workflow to enhance detection of infectious diseases.
SARS-CoV-2
Other pathogens
The RT-LAMP protocol and application note includes standalone reagents for fast and simple testing and surveillance of viral pathogens, including SARS-CoV-2. It serves as a template for testing other targets using relevant primer sets.
RT-LAMP master mix reagents for faster and simpler detection of RNA targets with maximum flexibility to optimize viral pathogen research for SARS-CoV-2, measles and influenza assay development.
This innovative RUO kit utilizes non-invasive saliva or swab samples, delivers rapid results without use of any sophisticated equipment or personnel training, and can be used for frequent surveillance of SARS-CoV-2.
Resources
| Standalone enzymes | Invitrogen SuperScript IV RT-LAMP Master Mix | Invitrogen Colorimetric ReadiLAMP™ Kit,
| |
|---|---|---|---|
| Format | Catalog reagents | Master mix | Assay kit |
| Description | Protocol and Application note | Optimized enzymatic reaction master mix | Optimized colorimetric detection assay for SARS-CoV-2 |
| Application | Research & Surveillance | Research & Surveillance | Research & Surveillance |
| Optimal reaction temperature | 60°C | 65°C | 65°C |
| Reaction time | 30-50 min | 10-30 min | 30-60 min (Direct LAMP option available) |
| Polymerase | Bsm DNA Polymerase | Proprietary Bst mutant | Proprietary Bst mutant |
| Reverse transcriptase | SuperScript IV | SuperScript IV | SuperScript IV |
| Additional additives | Yes | Yes | No |
| Dye | SYBR Green I and SYTO 9 / fluorescence | SYTO 9 / fluorescence | Colorimetric indicator |
| Sample | Purified RNA/DNA | Purified RNA/DNA | Purified RNA or Direct |
| SARS-CoV-2 specific primers and controls included | No | No | Yes |
| Other than SARS targets | Yes | Yes | No |
| Sophisticated instruments | Optional (Heat block or thermal cycler) | Optional (Heat block or thermal cycler) | No (Heat block) |
Application notes
Flyers
Application notes
Flyers
For Research Use Only. Not for use in diagnostic procedures.