While the term immunohistochemistry (IHC) is often used interchangeably with immunocytochemistry (ICC), significant differences exist between IHC and ICC in terms of the biological sample that is analyzed. Simply put, IHC is performed on samples derived from tissues that have been histologically processed into thin sections and the staining process exploits enzymes which catalyze the deposition of a colored staining product at antigenic sites within the sample. ICC relies on the same enzyme reactions as IHC, but it is performed on samples consisting of cells grown in a monolayer or cells in suspension which are deposited on a slide. However, on our website and elsewhere, it is much more common to see ICC used generically to describe immunofluorescence (IF) staining of tissue sections or cells.
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Sample source
Prior to IHC, tissues are removed from the patient or animal and either frozen or chemically preserved (fixed) and then embedded in paraffin wax. Sections as thin as 4 μm are sliced from frozen or paraffin-embedded tissue and mounted onto slides in preparation for antibody-mediated staining. In this way, researchers can look at the localization of the target antigens within cellular components while maintaining the original architecture of the surrounding tissue, as shown in the right panel below.
For ICC, most, if not all, of the extracellular matrix and other stromal components are removed, leaving only whole cells, as shown in the left panel below. Sample sources for ICC can be from any suspension of cells, obtained from patients or animals (e.g., blood smears, swabs and aspirates) or cultured cells grown in monolayers, usually on sterile glass coverslips. The latter is the most common sample type used for ICC. As mentioned above, ICC is also synonymous with IF staining, especially when we present results with cultured cells. The 2 abbreviations are often used interchangeably in this context.
Comparison of immunocytochemistry (ICC) and immunohistochemistry (IHC) staining. A549 human adenocarcinoma cells (left panel; ICC) and a human colon carcinoma tissue section (right panel; IHC) were fixed and stained for protein phosphatase 2 (PP2A) using an unconjugated mouse anti-human PP2A primary antibody and an HRP-conjugated goat anti-mouse secondary antibody. Enzyme-mediated staining was completed using our Metal Enhanced DAB Substrate Kit (34065), resulting in brown deposits at sites where PP2A was localized.
Learn more: IHC Troubleshooting Guide
Besides the biological source, IHC and ICC differ in the amount of sample processing required before antibody-mediated staining. For example, ICC is associated with whole cells that have to be permeabilized, either through a fixation procedure or a separate permeabilization step, to facilitate antibody penetration to the intracellular targets. Depending on the thickness of the section and the method of fixation, IHC samples may not have to undergo a separate permeabilization step. However, most formalin-fixed, paraffin-embedded (FFPE) IHC sections must be further processed prior to antibody staining to uncover latent epitopes on antigenic targets. This is usually referred to as epitope or antigen retrieval.
Once the samples are processed, there are few differences in the antibody staining protocol between IHC and ICC, although, as with all antibody-based staining, the protocol must be optimized based on the nature of the samples to be stained and the antibodies used.
Learn more: Fixation Strategies and Formulations used in IHC
- Coons, A.A., et al. (1942) J Immunol 45, 159-170.
- Hassel, J. and Hand, A.R. (1974). J. Histochem. Cytochem. 22 229-239.
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