Tools for assembling multiple DNA Fragments to build large and seamless clones
GeneArt Seamless Cloning and GeneArt Gibson Assembly Kits allow for the simultaneous assembly of up to 15 DNA fragments to create precise, very large constructs with no unwanted sequences in highly efficient reactions. These cloning methods circumvent the need for multiple rounds of restriction enzyme analysis and digestion, DNA end-repair, de-phosphorylation, ligation, enzyme inactivation and clean-up, and loss of precious DNA saving 3-4 weeks versus traditional RE cloning methods.
Which seamless cloning kit is right for you?
| Order now | Order now | Order now | Order now | Order now | |
| Assemble up to 4 fragments | Assemble up to 8 fragments | Assemble up to 15 fragments | |||
|---|---|---|---|---|---|
| GeneArt Seamless Cloning Enzyme Mix | GeneArt Seamless Cloning & Assembly Kit | GeneArt Seamless PLUS Cloning and Assembly Kit | GeneArt Type IIs Assembly kits | GeneArt Gibson Assembly HiFi and EX Kits | |
| Cloning efficiency | >90% |
| >90% |
| >90% |
| All-in-one enzyme/buffer mix | |||||
| Number of fragments cloned simultaneously | Up to 4 | Up to 4 | Up to 4 | Up to 8 | Up to 15 |
| Maximum assembled product size | Up to 13 kb (with vector) | Up to 13 kb (with vector) | Up to 40 kb (with vector) |
| Up to 100 kb+ (with vector) |
| Time to clone multiple fragments | Typically in half a day | 1 day | Typically in half a day | Typically in half a day | Typically in half a day |
| Assemble fragments w/o end sequence homology w/o PCR | |||||
For assembly using S. cerevisiae MaV203 see the GeneArt High-Order Genetic Assembly System.
Resources
Support
Cloning Support Center
Find tips, troubleshooting help, and resources for your cloning applications
Gibson Assembly® is a registered trademark of SGI-DNA, Inc. used under permission and license.
For Research Use Only. Not for use in diagnostic procedures.