The articles listed below comprise our application notes, scientific posters, and BioProbes Journal articles collections. These resources give overviews of specific flow cytometry applications and as well as experimental design details and data and results.
Articles that detail flow cytometry applications
Featured articles
Copper-safe Click-iT Plus EdU Proliferation Assay: Improved compatibility with simultaneous phycobiliprotein and fluorescent protein detection
This scientific poster demonstrates how the Click-iT Plus EdU Proliferation assay can be used with classic fluorescent proteins and phycobiliproteins for cell proliferation detection, eliminating the need for using cumbersome antibody-based Anti-BrdU assays.
Multiparameter analysis of murine regulatory T cells and dendritic cells with the Attune NxT Flow Cytometer
This application note describes the use of the Attune NxT Flow Cytometer for 4-laser and 10-color immunophenotyping analysis of stained mouse splenocytes using a fixation and permeabilization protocol.
Search all application notes
| Type | Title | Category |
|---|---|---|
| Application note (2019) | Designing a T cell backbone panel using the Attune NxT Flow Cytometer | Attune/Attune NxT Flow Cytometer, flow cytometer, flow cytometry, immunophenotyping, multicolor flow cytometry, fluorescent dyes |
| Application note (2019) | The importance of accurate cell counting in flow cytometry and cell sorting | flow cytometer/flow cytometry, viability, cell counting, automated cell counter, Countess |
| Application note (2018) | A guide to analyze Attune NxT FCS files in ModFit LT software | Attune/Attune NxT Flow Cytometer, flow cytometer, flow cytometry, Attune NxT Software |
| Application note (2018) | A guide to analyzing Attune NxT FCS data files using FlowJo software | Attune/Attune NxT Flow Cytometer, flow cytometer, flow cytometry, Attune NxT Software |
| Application note (2018) | High-throughput automation with the Attune NxT Autosampler: consistent results across all wells and across plates | Attune/Attune NxT Flow Cytometer, flow cytometer, flow cytometry, multicolor flow cytometry, Autosampler |
| Application note (2018) | Using the Attune NxT Flow Cytometer for rapid and accurate analysis of nuclear DNA content in plants | Attune NxT Flow Cytometer, plants |
| Application note (2018) | High-throughput automation with the Attune NxT Autosampler: consistent results across all wells and across plates | Attune/Attune NxT Flow Cytometer, Autosampler |
| Application note (2018) | A guide to analyze Attune NxT FCS data files in FCS Express 6 Software | Attune/Attune NxT Flow Cytometer, Attune NxT software, flow cytometry |
| Application note (2017) | Recommendations for accurate concentration measurements on the Attune NxT Flow Cytometer | Attune/Attune NxT Flow Cytometer, flow cytometry, cell concentration, cell counting |
| Application note (2017) | Flow cytometry analysis of transcription factor expression during differentiation of hPSC-derived cardiomyocytes | Attune/Attune NxT Flow Cytometer, cell differentiation, flow cytometry, multicolor flow cytometry, stem cell research |
| Application note (2017) | Validated 10-color T cell panel using the Attune NxT Flow Cytometer | antibodies, Attune/Attune NxT Flow Cytometer, flow cytometry, immunology, immunophenotyping, fluorescent dyes, multicolor flow cytometry |
| Application note (2017) | Flow cytometry analysis of dose response for apoptosis induction | apoptosis, Attune NxT, Autosampler, flow cytometry, cell toxicity, CellEvent Caspase-3/7 Green |
| Application note (2017) | Recommendations for accurate carryover measurements on the Attune NxT Flow Cytometer | Attune/Attune NxT Flow Cytometer, flow cytometer, flow cytometry, cell concentration, cell counting |
| Application note (2016) | Detection of fluorescent proteins using the Attune NxT Flow Cytometer | Attune/Attune NxT Flow Cytometer, flow cytometer, flow cytometry, multicolor flow cytometry, fluorescent dyes, fluorescent protein |
| Application note (2016) | Detection of platelets in whole blood using the Attune NxT Flow Cytometer | Attune NxT, flow cytometer, platelets, immunophenotyping, no-wash, no-lyse |
| Application note (2015) | Attune NxT Flow Cytometer for 6-color immunophenotyping analysis of stained human whole blood using a no-lyse, no-wash protocol, with no compensation | Attune NxT, flow cytometer, immunophenotyping, multicolor flow cytometry |
| Application note (2015) | Detection of murine regulatory T cells on the Attune NxT Flow Cytometer | Attune NxT, flow cytometer, immunophenotyping, multicolor flow cytometry |
| Application note (2015) | Multiparameter analysis of murine regulatory T cells and dendritic cells with the Attune NxT Flow Cytometer | Attune NxT, flow cytometer, immunophenotyping, multicolor flow cytometry |
| Application note (2015) | No-wash, no-lyse detection of leukocytes in human whole blood on the Attune NxT Flow Cytometer | Attune/Attune NxT Flow Cytometer, flow cytometer, flow cytometry, immunophenotyping, multicolor flow cytometry, fluorescent dyes |
| Application note (2015) | Ten-parameter immunophenotyping of human lysed whole blood with the Attune NxT Flow Cytometer allows identification of multiple T cell subsets and myeloid cells | Attune NxT, flow cytometer, immunophenotyping, multicolor flow cytometry |
| Application note (2015) | 13-parameter immunophenotyping of human lysed whole blood with the Attune NxT Flow Cytometer allows for the identification of B cells, NK cells, multiple T cell subsets and myeloid cells | Alexa Fluor, antibodies, Attune NxT, compensation, flow cytometer, fluorescent dyes, immunophenotyping, multicolor flow cytometry, nanocrystals, Qdot |
| Application note (2015) | Live/dead algal analysis with the Attune NxT Flow Cytometer | algae, Attune NxT, flow cytometer, flow cytometry assays, fluorescent dyes, microbiology, viability |
| Application note (2015) | No-wash, no-lyse detection of phagocytic cells via a pHrodo BioParticles functional assay in human whole blood on the Attune NxT Flow Cytometer | antibodies, Attune NxT, flow cytometer, flow cytometry sample preparation, fluorescent dyes, immunology, immunophenotyping, particles, pH detection |
| BioProbes articles (Issues 50– present day) | BioProbes Journal of Cell Biology Application | cell analysis, flow cytometry, imaging microscopy, immunoassays, antibodies, protein detection and quantification |
| Blog post | Clog resistance of non-pressure based flow cytometers | Attune NxT, flow cytometer |
| Blog post | Super Bright conjugates for flow cytometry | antibodies, Attune NxT, flow cytometry, immunophenotyping, multicolor flow cytometry, Super Bright |
| Blog post | What are event rates and should you always trust the “Maximum Event Rate” of your flow cytometer? | Attune NxT, flow cytometer, instrument set-up |
| Blog post | Flow cytometer fluidics and why they are not all the same | Attune NxT, flow cytometer, instrument set-up |
| Fluorophore | Pacific Blue dye | antibody conjugates, flow cytometry, immunophenotyping, violet laser |
| Fluorophore | Pacific Green dye | antibody conjugates, flow cytometry, immunophenotyping, violet laser |
| Fluorophore | Pacific Orange dye | antibody conjugates, flow cytometry, immunophenotyping, violet laser |
| Fluorophore | Super Bright 436 dye | antibody conjugates, flow cytometry, immunophenotyping, tandem polymer dyes, violet laser |
| Fluorophore | Super Bright 600 dye | antibody conjugates, flow cytometry, immunophenotyping, tandem polymer dyes, violet laser |
| Fluorophore | Super Bright 645 dye | antibody conjugates, flow cytometry, immunophenotyping, tandem polymer dyes, violet laser |
| Fluorophore | Super Bright 702 dye | antibody conjugates, flow cytometry, immunophenotyping, tandem polymer dyes, violet laser |
| Fluorophore | Allophycocyanin (APC) | antibody conjugates, flow cytometry, immunophenotyping, phycobiliproteins, tandem dyes |
| Fluorophore | R-phycoerythrin (R-PE) | antibody conjugates, flow cytometry, immunophenotyping, phycobiliproteins, tandem dyes |
| Fluorophore | Cyan fluorescent protein (CFP) | flow cytometry, fluorescent proteins |
| Fluorophore | Green fluorescent protein (GFP) | flow cytometry, fluorescent proteins |
| Fluorophore | Red fluorescent protein (RFP) | flow cytometry, fluorescent proteins |
| Fluorophore | Fluorophore selection guide | antibody conjugates, flow cytometry, immunophenotyping |
| Immunology information | B cell activation functional assay | immunology |
| Immunology information | Costimulatory and inhibitory interactions control T cell response | immunology, stimulation, T cells |
| Immunology information | Foxp3 overview | flow cytometry, Foxp3,intracellular staining |
| Immunology information | Innate immunity overview | immunology |
| Immunology information | Innate lymphoid cells overview | immunology |
| Immunology information | Microbiome (Microbiota) overview | immunology |
| Immunology information | Natural killer cells overview | immunology |
| Immunology information | T cell activation functional assay | immunology |
| Immunology information | T follicular helper differentiation functional assay | immunology |
| Immunology information | T reg differentiation functional assay | immunology |
| Immunology information | T regulatory cells overview | immunology |
| Immunology information | Th1 differentiation functional assay | immunology |
| Immunology information | Th17 cells overview | immunology |
| Immunology information | Th17 differentiation functional assay | immunology |
| Immunology information | Th2 differentiation functional assay | immunology |
| Immunology information | Th22 differentiation functional assay | immunology |
| Immunology information | Th9 differentiation functional assay | immunology |
| Immunology information | Toll-like receptor overview | |
| Molecular Probes Handbook | Phycobiliproteins—Section 6.4 | antibody labeling, multicolor flow cytometry, tandem conjugates |
| Molecular Probes Handbook | Microspheres—Section 6.5 | flow cytometer calibration, fluorescence microscopy/fluorescence imaging, microscope calibration, particles |
| Molecular Probes Handbook | Flow cytometry reference standards—Section 23.2 | flow cytometer |
| Molecular Probes Handbook | Using the Fluorescence SpectraViewer—Note 23.1 | flow cytometry, fluorescence, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins |
| Molecular Probes Handbook | MHC tetramer technology—Note 7.2 | antibodies, flow cytometry, immunology, Major Histocompatibility Complexes, T cells |
| Molecular Probes Handbook | Probes for mitochondria—Section 12.2 | antibodies, cell structure-mitochondria, CellLight, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes |
| Molecular Probes Handbook | Probes for lysosomes, peroxisomes and yeast vacuoles—Section 12.3 | antibodies, cell structure-lysosomes, CellLight, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes |
| Molecular Probes Handbook | Probes for the nucleus—Section 12.5 | antibodies, cell structure-nucleus, CellLight, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, membrane permeability |
| Molecular Probes Handbook | Probes useful at near-neutral pH—Section 20.2 | BCECF, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, pH detection, pH indicators, SNARF |
| Molecular Probes Handbook | Probes useful at acidic pH—Section 20.3 | flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, live-cell imaging, LysoSensor, pH detection, pH indicators, pHrodo |
| Molecular Probes Handbook | pH indicator conjugates—Section 20.4 | flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, live-cell imaging, LysoSensor, pH detection, pH indicators, pHrodo |
| Molecular Probes Handbook | Overview of pH indicators—Section 20.1 | fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, live-cell imaging, LysoSensor, pH detection, pH indicators, pHrodo |
| Molecular Probes Handbook | Nucleic acid stains—Section 8.1 | cell cycle, cell structure-nucleus, cell structure-plasma membrane, cell viability, DAPI, DNA binding dyes, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, membrane permeability, RNA binding dyes, SYTO, SYTOX |
| Molecular Probes Handbook | Tracers for membrane labeling—Section 14.4 | cell structure-plasma membrane, cell tracking, flow cytometry, fluorescence microscopy/fluorescence imaging, live-cell imaging |
| Molecular Probes Handbook | Microspheres and Qdot Nanocrystals for tracing—Section 14.6 | blood flow, FluoSpheres, neuronal tracing, particles, Qdot, Qtracker, quantum dots |
| Molecular Probes Handbook | Viability and cytotoxicity assay reagents—Section 15.2 | cell health, cell viability and function, fluorescence microscopy/fluorescence imaging, microplate reader |
| Molecular Probes Handbook | Viability and cytotoxicity assay kits for diverse cell types—Section 15.3 | cell health, cell viability and function, fluorescence microscopy/fluorescence imaging, microplate reader |
| Molecular Probes Handbook | Assays for apoptosis and autophagy—Section 15.5 | apoptosis, autophagy, flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging |
| Molecular Probes Handbook | Zenon Technology: Versatile reagents for immunolabeling—Section 7.3 | antibody labeling, flow cytometry, fluorescence microscopy/fluorescence imaging, secondary detection, Zenon |
| Pathways | Adaptive immunity pathways | immunology |
| Pathways | Innate immunity pathways | immunology |
| Pathways | Inflammatory response pathway | |
| Pathways | Interferon (IFN) cell signaling pathway | |
| Pathways | T-cell receptor (TCR) cell signaling pathway | |
| Pathways | Toll-like receptor (TLR) cell signaling pathway | |
| Protocols | Annexin V staining protocol for flow cytometry (Invitrogen eBioscience reagents) | apoptosis, flow cytometry |
| Protocols | BrdU Staining Protocol for Flow Cytometry (Invitrogen eBioscience reagents) | cell proliferation, flow cytometry |
| Protocols | Cell preparation for flow cytometry protocols (Invitrogen eBioscience reagents) | flow cytometry, sample preparation |
| Protocols | CellTrace CFSE cell proliferation Kit protocol | cell proliferation, flow cytometry |
| Protocols | CellTrace Far Red cell proliferation Kit protocol | cell proliferation, flow cytometry |
| Protocols | CellTrace Violet cell proliferation Kit protocol | cell proliferation, flow cytometry |
| Protocols | LIVE/DEAD Fixable Dead cell stains protocol | flow cytometry, viability |
| Protocols | LIVE/DEAD Sperm Viability Kit flow cytometry protocol | flow cytometry, viability |
| Protocols | LIVE/DEAD Violet Viability/Vitality Kit protocol | flow cytometry, viability |
| Protocols | OneComp and UltraComp Compensation Beads protocols for flow cytometry (Invitrogen eBioscience reagents) | compensation, flow cytometry |
| Protocols | Pharmacological Induction of Apoptosis with Camptothecin (Invitrogen eBioscience reagents) | apoptosis, flow cytometry |
| Protocols | Red blood cell lysis protocols using eBioscience lysis buffers (Invitrogen eBioscience reagents) | flow cytometry, sample preparation |
| Protocols | Staining cell surface targets for flow cytometry (Invitrogen eBioscience reagents) | extracellular staining, flow cytometry, immunophenotyping |
| Protocols | Staining cells with eFluor proliferation dyes for flow cytometry (Invitrogen eBioscience reagents) | cell proliferation, flow cytometry |
| Protocols | Staining intracellular antigens for flow cytometry (Invitrogen eBioscience reagents) | flow cytometry, immunophenotyping, intracellular staining |
| Protocols | SYTOX Dead Cell Stains protocol | cell viability, flow cytometry |
| Protocols | Super Bright staining buffer protocol | antibody conjugates, flow cytometry, immunophenotyping |
| Protocols | Viability staining protocol for flow cytometry (Invitrogen eBioscience reagents) | cell viability, flow cytometry |
| Scientific poster (SITC 2023) | Time-resolved analysis of CAR-T Cell induced apoptosis of low frequency target cells using an image-enhanced flow cytometer was enabled by fluorescent protein compensation with BrightComp eBeads | flow cytometry, Brightcomp ebeads |
| Scientific poster (CYTO / AAI 2023) | Conjugation tricks: optimization of antibody labeling with novel DNA-based fluorescent labels | NovaFluor antibodies , flow cytometry, antibody conjugation |
| Scientific poster (CYTO / AAI 2023) | Expanding the capacity of complex spectral panels by using NovaFluor-conjugated antibodies | flow cytometry , NovaFluor, complex spectral panels |
| Scientific poster (CYTO / AAI 2023) | Harnessing violet and ultraviolet fluorophores for flexibility in T cell panel design | flow cytometry , Brilliant Ultra Violet and Brilliant Violet |
| Scientific poster (CYTO / AAI 2023) | Identifying and sorting mouse progenitor cells with spectral flow cytometry | flow cytometry, spectral flow cytometry, cell sorting |
| Scientific poster (CYTO / AAI 2023) | Impact of fixative type on flow cytometry fluorochrome fluorescence | flow cytometry |
| Scientific poster (CYTO / AAI 2023) | Improved reproducibility and repeatability in full spectrum absolute cell counting | flow cytometry, absolute counting beads, CountBright Plus Beads |
| Scientific poster (CYTO / AAI 2023) | Investigation of problematic dye combinations to inform optimal panel design in spectral cytometry | flow cytometry , optimal panel design, spectral flow cytometry |
| Scientific poster (CYTO/AAI 2023) | New generation of fluorescent probes for cell-based measurements of caspase activation and mitochondrial superoxide | flow cytometry, CellEvent Caspase, MitoSox |
| Scientific poster (CYTO / AAI 2023) | Panel design by numbers quantitative evaluation of spillover, spread, and cross excitation to build to more colors | flow cytometry , panel design, spillover, spectral spread, NovaFluor antibodies |
| Scientific poster (CYTO/AAI 2023) | Systematic analysis of spectral mismatch between compensation particles and cell in conventional and spectral flow cytometry | flow cytometry, compensation beads, UltraComp eBeads Plus |
| Scientific poster (CYTO / AAI 2023) | Target MFI based voltage settings increase spectral resolution and simplifies workflow | flow cytometry, spectral cell sorter, Bigfoot sorter |
| Scientific poster (2019) | Development of in vitro immune effector function assays to better approximate the in vivo behavior of biotherapeutics and cell therapies | immune, immunology, flow cytometry, flow cytometer, attune, cell therapy, biotherapeutics |
| Scientific poster (2019) | Functional Flow Cytometry to predict PD-L1 Conformational Change and Improve Cancer Immunotherapy | flow cytometry, flow cytometer, attune, DyeCycle violet, PD-L1, cancer, immunotherapy, NLNW |
| Scientific poster (2019) | Expression of an immune checkpoint receptor VSIG4 defines new subsets of mouse peritoneal macrophages | flow cytometry, flow cytometer, attune, immune checkpoint, VSIG4, macrophages, mouse peritoneal |
| Scientific poster (2018) | Induced pluripotent stem cell sorting, culture and differentiation to desired cell lineage | Attune/Attune NxT, cell counting, cell sorting, Countess, EVOS, fluorescence activated cell sorting, iSort Automated Cell Sorter, imaging microscopy, stem cell research |
| Scientific poster (2018) | Modular, defined assembly of antibody-based constructs using click chemistry | Alexa Fluor, antibody labeling, Attune/Attune NxT, CellInsight, drug discovery, drug research, high content analysis (HCA), SiteClick, spheroids |
| Scientific poster (2018) | Hypoxia measurements in live and fixed cells using fluorescence microscopy, high-content imaging and flow cytometry | Attune/Attune NxT, brightfield microscopy, CellInsight, EVOS, high content analysis (HCA), hypoxia, spheroids |
| Scientific poster (2018) | Evaluation of multiple techniques for PMT optimization | Attune/Attune NxT Flow Cytometer, flow cytometer calibration, photomultiplier settings |
| Scientific poster (2018) | PUBLISH OR PERISH? Initiating, maintaining, and growing a publication history within an SRL role | flow cytometry, career advancement, shared lab resources |
| Scientific poster (2018) | Prospective cytometric study evaluating the alkaline phosphatase activity as a potential functional biomarker of primitive malignant cells in acute leukemia | antibodies, Attune/Attune NxT, cancer, cell function, flow cytometry, immunophenotyping, stem cell research |
| Scientific poster (2018) | Synergistic effect of curcumin in combination with proapoptotic drugs in human cell models for chronic (CLL) lymphocytic leukaemia | Attune/Attune NxT Flow Cytometer, cell cycle, cancer, drug development, drug research, flow cytometry |
| Scientific poster (2018) | Differently cell doublet profiles: comparison of light scattering with fluorescence measurement techniques | Attune/Attune NxT Flow Cytometer, cell counting, cell cycle, cell viability, flow cytometry, single cell discrimination |
| Scientific poster (2018) | Differential expression of PD-L1 on myeloid derived suppressor cells as a potential screening approach for cancer immunotherapy in multiple myeloma | Attune/Attune NxT, cancer, drug research, , immunology, immuno-oncology, multicolor flow cytometry, translational research solutions |
| Scientific poster (2018) | Maintaining even temperature distribution over chilled samples in 96-well plates and micro-centrifuge tubes | academic research solutions, microplates, quality control, temperature control |
| Scientific poster (2018) | A comparison of GFP BrightComp eBeads Compensation Bead Kit performance against multiple GFP variants | Attune/Attune NxT, beads, flow cytometry compensation, fluorescent protein detection, |
| Scientific poster (2018) | Fluorescent hypoxia sensor for flow cytometry | apoptosis, Attune/Attune NxT, brightfield microscopy, EVOS, flow cytometry, hypoxia, immunology |
| Scientific Poster (2017) | T-lymphocyte immunophenotyping: 14-color flow cytometry panel design using the Attune NxT Flow Cytometer and Super Bright fluorescent dyes | antibodies, Attune NxT, fluorescent dyes, immunophenotyping, multicolor flow cytometry, particles, Super Bright |
| Scientific Poster (2016) | A novel non-cytotoxic fluorescent dye for cell proliferation analysis in flow cytometry | Attune NxT, cell health, cell proliferation, CellTrace, EVOS, flow cytometry, fluorescence microscopy/fluorescence imaging |
| Scientific Poster (2016) | An objective test to qualify plate samplers for complex flow cytometric assays | Attune NxT, Autosampler, flow cytometer |
| Scientific Poster (2016) | Cell mediated cytotoxicity in “untouched” whole blood | Attune NxT, cell concentration, cell cycle, flow cytometry assays, viability, whole blood |
| Scientific Poster (2016) | No-lyse no-wash new strategy for CD34+ for absolute cell counting without beads | antibodies, Attune NxT, cell counting, compensation, flow cytometry assays , fluorescent dyes |
| Scientific Poster (2015) | A no lyse, no-wash approach to characterizing phagocyte phenotype and function in whole human blood on the Attune NxT Flow Cytometer | Attune NxT, cell cycle, flow cytometer, flow cytometry assays, immunophenotyping, no lyse, phagocytosis, whole blood |
| Scientific poster (2014) | Copper-safe Click-iT Plus EdU proliferation assay: Improved compatibility with simultaneous phycobiliprotein and fluorescent protein detection | cell proliferation, Click-iT, flow cytometry, immunophenotyping |
| Scientific poster (2014) | Flow cytometry multiplexing used to analyze metabolic activity and assess pharmaceutical compound toxicity as a high-throughput screening tool | Attune NxT, drug discovery, flow cytometer, flow cytometry assay |
| Scientific Poster (2014) | Click-iT EdU Plus for flow cytometry: enhanced compatibility for multiplexing | Attune NxT, cell proliferation, Click-iT, multicolor flow cytometry |
| Scientific poster (2013) | Cell-based measurements of oxidative stress with a new near infrared emitting probe for reactive oxygen species: Applications for multiplexed evaluation of cell health | apoptosis, ArrayScan, caspase substrates, cell health, cells, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, high content analysis, microplate reader, oxidative stress |
| Scientific Poster (2013) | Cells coming to life: Tools for visualizing immune response | endocytosis/phagocytosis, flow cytometry, fluorescence microscopy/fluorescence imaging, live-cell imaging, pH detection, T cell stimulation |
| Scientific poster (2013) | Improved click chemistry demonstrating EdU cell proliferation with GFP-expressing cells and R-PE-based immunophenotyping | Alexa Fluor, cell cycle, cell proliferation, Click-iT, flow cytometry assays, fluorescent dyes, fluorescent proteins, immunophenotyping |
| Scientific poster (2013) | Monitor caspase 3/7 activity without cell fixation: A novel apoptosis reagent | Alexa Fluor, apoptosis, Attune NxT, caspase substrates, cells, fluorescent dyes, multicolor flow cytometry |
| Scientific poster (2013) | Reactive oxygen probes—A broad range of colors with easier labeling and compatibility with fixation: Novel CellROX reagents | cells, fluorescent dyes, multicolor flow cytometry, oxidative stress |
| Scientific poster (2013) | Reactive oxygen probes—A broad range of colors with easier labeling: Novel CellROX reagents | cells, fluorescent dyes, multicolor flow cytometry, oxidative stress, viability |
| Scientific poster (2011) | Rare event analysis: Detection of mouse plasmacytoid dendritic cells and human circulating endothelial cells | Alexa Fluor, endothelial cells, flow cytometry, fluorescent dyes, rare event detection |
| Scientific poster (2011) | The next step in the analysis of lymphocyte proliferation | Alexa Fluor, antibodies, Attune NxT, cell proliferation, CellTrace, flow cytometry assays, fluorescent dyes, live-cell imaging, violet laser-excited reagents |
| Scientific poster (2010) | A comparison of three techniques to induce efficient ex vivo T cell expansion | Alexa Fluor, antibodies, cell proliferation, CellTrace, flow cytometry, fluorescent dyes, immunology, magnetic beads, multicolor flow cytometry, viability |
| Scientific poster (2010) | Analysis of proliferation dynamics in primary and immortalized human cells | Alexa Fluor, cancer, cell proliferation, CellTrace, circulatory system, Click-iT, flow cytometry, fluorescent dyes, fluorescent proteins, nucleic acid quantitation |
| Scientific poster (2010) | Dual pulse labeling using EdU and BrdU to monitor alterations in cellular proliferation by fluorescence microscopy and flow cytometry | Alexa Fluor, cell cycle, cell proliferation, cells, Click-iT, drug discovery, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes |
| Scientific poster (2010) | Functional characterization of a novel fluorescent dye for proliferation analysis | Alexa Fluor, cell proliferation, cells, CellTrace, flow cytometry, fluorescent dyes, violet laser-excited reagents |
| Scientific poster (2010) | Non-cytotoxic near-IR DNA stain for cell cycle analysis in living cells | cell cycle, flow cytometry, fluorescent dyes, fluorescent proteins, live-cell imaging, nucleic acid labeling, viability |
| Scientific poster (2010) | Proliferative and phenotypic characterization of human mesenchymal stem cells by flow cytometry and imaging | Alexa Fluor, cell proliferation, cells, Click-iT, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, immunofluorescence (IF), immunophenotyping, stem cell research, violet laser-excited reagents |
| Scientific poster (2010) | Simultaneous analysis of cell death mechanisms and oxidative stress using Molecular Probes next generation reagents for imaging and flow cytometry | apoptosis, autophagy, caspase substrates, cells, flow cytometry/flow cytometer, fluorescence microscopy/fluorescence imaging, fluorescent dyes, live-cell imaging, oxidative stress |
| Scientific poster (2010) | Use of surface-modified microspheres for setting compensation in multicolor flow cytometry | antibodies, cells, flow cytometry compensation, fluorescent dyes, multicolor flow cytometry, particles, violet laser-excited reagents |
| Scientific poster (2009) | A comparative study of 25 different CD4 conjugates | Alexa Fluor, antibodies, fluorescent dyes, immunophenotyping, multicolor flow cytometry, nanocrystals, Qdot |
| Scientific poster (2009) | A novel xeno-free and serum-free culture medium for the expansion of human mesenchymal stem cells | cell culture, cell expansion, flow cytometry, fluorescent dyes, gene expression, immunophenotyping, stem cell research |
| Scientific poster (2009) | A simple, fast and quantitative single-step dead-cell indicator for flow cytometry | apoptosis, fluorescent dyes, multicolor flow cytometry, nucleic acid quantitation, viability |
| Scientific poster (2009) | Dead cell stains in flow cytometry: A comprehensive analysis | apoptosis, cells, flow cytometry, fluorescent dyes, immunophenotyping, viability |
| Scientific poster (2009) | Dual pulse labeling of S-phase population using click chemistry to measure changes in cell proliferation | Alexa Fluor, cancer, cell cycle, cell proliferation, cells, Click-iT, flow cytometry, fluorescent dyes |
| Scientific poster (2009) | Dual pulse labeling using a new thymidine analog 5-ethynyl-2’-deoxyuridine (EdU) to detect alterations in S-phase progression by fluorescence microscopy and flow cytometry | Alexa Fluor, cell cycle, cell proliferation, cells, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes |
| Scientific poster (2009) | Proliferative and phenotypic characterization of human mesenchymal stem cells by flow cytometry | Alexa Fluor, cell proliferation, cells, Click-iT, flow cytometry, fluorescent dyes, fluorescent proteins, immunophenotyping, nucleic acid quantitation, stem cell research, violet laser-excited reagents |
| Scientific poster (2008) | A new 405 nm excitable fluorescent probe of intracellular thiols for imaging and flow cytometric assays | ArrayScan, cells, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, glutathione, microplate reader, violet laser-excited reagents |
| Scientific poster (2008) | Characterization of DNA content, cyclin B1 and phosphorylated histone H3 with direct S-phase using EDU incorporation in multiparameter testing of cell lines with cell cycle blocking agents | Alexa Fluor, antibodies, apoptosis, ArrayScan, cell cycle, cell proliferation, cells, Click-iT, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes |
| Scientific poster (2008) | Detection of DNA synthesis by automated microscopy and image analysis: Comparison of BRDU method and a new click chemistry-based EDU method | Alexa Fluor, antibodies, ArrayScan, cell cycle, cell proliferation, cells, Click-it, flow cytometry, fluorescent dyes, microplate reader, nucleic acid labeling |
| Scientific poster (2008) | Detection of S-phase cell cycle progression using 5-ethynyl-2'-deoxyuridine incorporation with click chemistry | Alexa Fluor, antibodies, ArrayScan, cell cycle, cells, Click-iT, flow cytometry, fluorescent dyes, microplate reader, nucleic acid labeling, nucleic acid quantitation |
| Scientific poster (2008) | Effects of the thymidine analogues EdU and BrdU on cell viability and cycle progression | Alexa Fluor, cell cycle, cell proliferation, cells, Click-iT, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, viability |
| Scientific poster (2008) | Zenon labeling technology facilitates detection of intra-cellular phosphoprotein determinants | Alexa Fluor, antibodies, cell signaling, cells, flow cytometry, fluorescent dyes, protein labeling, rare cell detection, Zenon |
| Scientific poster (2007) | A new approach for the detection of intracellular glutathione by fluorescence microscopy and flow cytometry | ArrayScan, cells, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, glutathione, live-cell imaging, microplate reader, viability, violet laser-excited reagents |
| Scientific poster (2007) | Evaluation of click chemistry-based alternative to BrdU antibody labeling in tissue and cultured cells using fluorescence microscopy and flow cytometry | antibodies, ArrayScan, cell cycle, cell proliferation, cells, Click-iT, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, microplate reader, nucleic acid labeling |
| Scientific poster (2006) | Novel violet-excited reagents for detection of viability and vitality | Alexa Fluor, cell health, CellTrace, flow cytometry, fluorescent dyes, viability, violet laser-excited reagents |
| Selection guide | Antibody fixation considerations | sample preparation |
| Selection guide | Human antibody cross reactivity chart | antibody cross-reactivity, flow cytometry |
| Selection guide | Mouse antibody cross reactivity chart | antibody cross-reactivity, flow cytometry |
| Selection guide | Cytokine bioassays guide | cytokines, flow cytometry, immunophenotyping, intracellular staining |
| Selection guide | Intracellular staining guide (for mouse & human cytokines and non-cytokine proteins) | cytokines, flow cytometry, intracellular staining |
| Selection guide | Human CD & other cellular antigens | flow cytometry, immunology, immunophenotyping, extracellular staining |
| Selection guide | Intracellular staining buffer selection guide | flow cytometry, immunophenotyping, intracellular staining |
| Selection guide | Mouse CD & other cellular antigens | flow cytometry, immunology, immunophenotyping, extracellular staining |
| Selection guide | Phospho flow cytometry antibody clone buffer selection guide | flow cytometry, immunophenotyping, intracellular staining, phosphoflow cytometry |
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.