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Vanquish Trap Heart-Cut 2D-LC Systems

Simplified multi-dimensional LC method development.

Vanquish Trap Heart-Cut 2D-LC Systems

Achieve in-depth analysis for greater confidence in peak purity and identity with the Thermo Scientific Vanquish Trap Heart-Cut 2D-LC System. This two-dimensional LC (LC-LC) system enables you to collect a single fraction of the first-dimension separation onto a trap column to pre-concentrate, and/or achieve mobile phase compatibility for transferring the fraction for a second-dimension separation or compatibility between UV and MS detection. Heart-cut 2D-LC can reveal hidden impurities that co-elute with the analytes of interest by applying two complementary column chemistries to gain more separation power. The system is configured using modules from the Vanquish Flex UHPLC or Horizon UHPLC systems, delivering performance, robustness and ease of use of the Vanquish LC platform.


Highlights

Greater confidence

Collect single or multiple fractions of the first-dimension separation onto a trap column and transfer the cut-out fraction(s) to a second-dimension column with complementary column chemistry in an automated workflow for more confidence in peak purity.

Increased sensitivity

The trap column enables enrichment of low-abundant analytes and removal of diluting or disturbing solvents from the eluent by trapping and pre-concentrating the analytes of interest to increase sensitivity.

Simplified method development

The second-dimension eluent provides the flow for solvent modulation, such as eluent strength reduction or buffer exchange of the first-dimension fraction. This process can address solvent incompatibility of UV and MS methods or mobile phase incompatibility between different column chemistries to support multiple combinations of 1D and 2D methods. 

Save time and resources

Automated workflows, with predefined trap and elution steps, allow for unattended sample analysis while reducing human error, saving time and bench space compared to offline approaches.

Flexibility

The modularity of the Vanquish LC systems makes it easy to build a system to best meet your application requirements with flexibility to subsequently upgrade a standard HPLC instrument to a more advanced 2D-LC configuration for more complex analyses. 

Easy and reliable tool-free connections

The Vanquish Trap Heart-Cut 2D-LC system is fitted with Thermo Scientific Viper fingertight fittings, which support near-zero dead volume connections and enable sharper peaks and tool-free setup.


What is trap-based heart-cut 2D-LC?

Our trap-based heart-cut 2D-LC system allows you to automate and cut out a single fraction eluting from the first dimension (1D) column onto a trap column. This allows for pre-concentrating low-abundant analytes and addresses solvent incompatibility issues before the fraction is eluted onto a second dimension (2D) column to resolve difficult or co-eluting peaks.

 

The flow scheme illustrates how the two six-port valves in combination with the 2D pump and the t-piece are used to reduce the solvent strength and to load the cut-out 1D fraction onto the trap column pre-concentrating the sample before being loaded on the second dimension (2D) column.

Flow scheme of the different steps in single heart-cut trapping on the trap column with subsequent solvent modulation (green fluidic pathway), washing, and analysis on the 2D column.


Videos


System specifications

The table below provides an overview of the Vanquish Trap Heart-Cut 2D-LC system specifications for standard setups. Not all specifications are listed. Specifications may differ based on the specific system components selected. See detailed Component Specifications for more information. 

Category Vanquish Trap Heart-Cut 2D-LC System
System pressure, maximum 1000 or 1500 bar, depends on system configuration
Flow range 0–5 or 0–8 mL/min, depends on pumps selected 
Pump options (default gradient delay volume)

Binary (35, 200 µL) 
Quaternary (679 µL) 

Delay volume depends on pump(s) selected

Biocompatible Yes
pH range 2–12, chloride concentration up to 1 mol/L
System design Modular
Touchscreen user interface display compatible (optional) Yes, depends on system configuration
Solvent monitoring system compatible (optional) Yes, depends on system configuration
Customizable gradient delay volume to simplify method transfer Yes, with metering device
SmartInject technology for maximum injection precision and improved column lifetime Yes
Injection volume range

Standard 0.01-25 µL

Optional: 0.01-100 µL

Sample cooling Yes, 4-40°C

Sample capacity (standard)

Based on standard 12 mm OD vials (≤ 1.5 mL)

4 racks of 54 vials (≤ 1.5 mL) each, other rack formats available

 + capacity of 12 × 22.5 mm OD vials (≤ 10 mL) in the carousel

Sampler extension available (optional) Yes, with Vanquish Charger
Column compartment thermostatting

Yes, 5-120 °C

Column compartment pre-heater

Active and passive 

Column compartment post-column cooler Yes
Compatible Detectors

Vanquish Charged Aerosol Detector 

Vanquish Diode Array Detector 

Vanquish Fluorescence Detector 

Vanquish Multiple Wavelength Detector 

Vanquish Variable Wavelength Detector

Refractive Index Detector

Mass Spectrometer


Component specifications

Standard Vanquish Trap Heart-Cut 2D-LC systems are made up of the following components. Download the associated specifications sheet for more detail on each component. 

  Vanquish Horizon Trap Heart-Cut 2D-LC System  Vanquish Flex Trap Heart-Cut 2D-LC System

Pump

 

Download Specification Sheet

Vanquish Binary Pump H
or 
   1 Vanquish Binary Pump H
and 
Vanquish Quaternary Pump F

 

Note: Binary pump recommended as ²D pump

Vanquish Binary Pump F
or 
Vanquish Binary Pump F
and 
Vanquish Quaternary Pump F
 

Note: Binary pump recommended as ²D pump

Autosampler

 

Download Specification Sheet

Vanquish Split Sampler HT Vanquish Split Sampler FT

Extended sample capacity (optional)

 

Download Specification Sheet

Vanquish Charger
(optional)

Column compartment

 

Download Specification Sheet

Vanquish Column Compartment H

Virtually zero dead volume system connections

 

Download Specification Sheet

Viper UHPLC Fittings Kits

Detectors

 

Visit our HPLC and UHPLC Detectors page

Contact us to get a quote or discuss an HPLC/UHPLC solution


For Research Use Only. Not for use in diagnostic procedures.

 

 

 

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