Next-generation sequencing sample preparation using the NanoDrop One Microvolume UV-Vis Spectrophotometer

Achieving accurate sequencing results requires a reliable nucleic acid quantity and purity assessment. Currently, qPCR serves as the gold standard for quantification method but is expensive, time consuming, and often requires vetting SOPs, yet without providing purity details. As a convenient and economical alternative to qPCR, the Thermo Scientific™ NanoDrop™ One/One^C Spectrophotometer provides a sensitive quantification measurement and identifies common nucleic acid contaminants that prevent sequencing success.

Watch and learn how the NanoDrop One/One^C Spectrophotometer, can efficiently and effectively analyze qPCR data for high-quality sequencing.

Key learning objectives

• Understand and review the NGS workflow
• Learn how to set up your sequencing experiments for success
• Explore the NanoDrop One/OneC Spectrophotometer as an alternative to qPCR quantification

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