NIH 3T3 fibroblast undergoing progressive depolarization induced by the stepwise increase of KCl concentration.
NIH 3T3 fibroblast undergoing progressive depolarization induced by the stepwise increase of KCl concentration from 5 mM (bottom panel) to 120 mM (top panel). The cell culture was loaded with DiSBAC2(3)) (Cat. no. B413) and examined under a confocal laser-scanning microscope after each appropriate medium change, keeping a constant plane of section (Exp Cell Res 231, 260 (1997)). The image was contributed by Rita Gatti, Institute of Histology and General Embryology, University of Parma, Parma, Italy.
