Human metaphase chromosome spreads.

Fluorescent probes generated with ARES DNA Labeling Kits hybridized to human metaphase chromosome spreads. Centromere probes specific for chromosomes 17, 1 and 15 were prepared by nick translation and labeled with kits containing green-fluorescent Alexa Fluor 488 (A21665), red-orange–fluorescent Alexa Fluor 546 (A21667) and red-fluorescent Alexa Fluor 594 (A21669) dyes. DNA was counterstained with the blue-fluorescent Hoechst 33342 dye (H1399, H3570, H21491), and the slides were mounted using the ProLong Antifade Kit (P7481). This multiple-exposure image was obtained with bandpass filter sets appropriate for fluorescein, rhodamine, Texas Red dye and DAPI.

Fluorescent probes generated with ARES DNA Labeling Kits hybridized to human metaphase chromosome spreads. Centromere probes specific for chromosomes 17, 1 and 15 were prepared by nick translation and labeled with kits containing green-fluorescent Alexa Fluor 488 (A21665), red-orange–fluorescent Alexa Fluor 546 (A21667) and red-fluorescent Alexa Fluor 594 (A21669) dyes. DNA was counterstained with the blue-fluorescent Hoechst 33342 dye (H1399, H3570, H21491), and the slides were mounted using the ProLong Antifade Kit (P7481). This multiple-exposure image was obtained with bandpass filter sets appropriate for fluorescein, rhodamine, Texas Red dye and DAPI.

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