Isolation of mitochondria using the Thermo Scientific Sorvall LYNX 6000 Superspeed Centrifuge
This procedure describes the isolation of highly pure mitochondria from large volume cultures of Saccharomyces cerevisiae using the Thermo Scientific Sorvall LYNX 6000 superspeed centrifuge. In addition, this procedure can be readily adapted to other cell types, including bacteria, insects, or mammalian cells.