mirVana miRNA Isolation kits use a rapid procedure to isolate total RNA, including small RNAs such as miRNA, small interfering RNA (siRNA), and small nuclear RNA (snRNA), from tissues and cells.
Applied Biosystems TaqMan Advanced miRNA Assays enable highly sensitive and specific quantification of mature miRNAs using qPCR. Together with the TaqMan Advanced miRNA cDNA Synthesis Kit, this solution is designed to provide a streamlined workflow that allows for exceptional detection of multiple miRNA targets from a single sample.
- Universal reverse transcription (RT)—one RT step for all TaqMan Advanced miRNA Assays.
- Sensitive— Detect targets with as few as 60 copies of input miRNA into the cDNA synthesis reaction.
- Specific—detect only mature miRNA and distinguish related highly homologous miRNAs with gold-standard TaqMan probe specificity.
- Small sample input—detect and quantify mature miRNA from as little as 1 pg of total RNA or 2 μL of purified plasma or serum.
- Versatile—compatible with tissue and biofluids including serum and plasma.
- Fast delivery—all pre-designed assays ship out in 1–2 business days
Already know your target of interest?
Search for a pre-designed TaqMan Advanced miRNA Assay using the tool below:
Looking to design your own assay? Click here to learn more about our offerings, and to request a design for a custom TaqMan Advanced miRNA Assay when a pre-designed assay is not offered.
Availability & sizes: Inventoried assays are pre-packaged in "Small" scale and are ready to ship.
Now available: TaqMan Advanced miRNA assays in card, plate, and OpenArray format
Enable highly sensitive and specific quantification of mature miRNAs using qPCR in higher throughputs.
Search for pre-designed array plates ›
Search for pre-designed array cards ›
Design your own custom array card ›
Taqman™ OpenArray Human Advanced MicroRNA Panel ›
Taqman Advanced miRNA Product Selection Guide
Format | Recommended TaqMan assay/array |
| Individual qPCR Assays | New TaqMan Advanced miRNA Assays All pre-designed assays are inventoried and ship out in 1-2 days |
96-well plate | TaqMan Advanced miRNA 96 well array plates |
| 384-well microfluidic TaqMan Array card | TaqMan Advanced miRNA array cards Search for predesigned 384-well arrays › |
| OpenArray plate | TaqMan Advanced miRNA OpenArray panels Run three samples per QuantStudio 12K Flex OpenArray plate, translating up to 818 assays per sample (up to 758 unique assays) Taqman™ OpenArray Human Advanced MicroRNA Panel Contact: QuantStudioFrontDesk@lifetech.com |
| Custom 384 well plates | Contact: specialty_plates@thermofisher.com |
Workflow
TaqMan Advanced miRNA cDNA Synthesis Kit
The TaqMan Advanced miRNA cDNA Synthesis Kit is used for preparation of cDNA templates from the entire pool of mature miRNAs in a sample.
miR-Amp Universal Amplification
miR-Amp uses universal primers and master mix to uniformly increase the amount of cDNA for each target. miR-Amp reagents are included in the TaqMan Advanced miRNA cDNA Synthesis Kit.
Applied Biosystems real-time PCR instruments
With Applied Biosystems real-time PCR platforms, you get true value with excellent performance, reliability, and world-class support. Designed for compatibility with Applied Biosystems TaqMan Assays and the flexibility of unlimited real-time chemistry choices, we make it easy for you to get started with your experiments.
mirVana miRNA Isolation kits use a rapid procedure to isolate total RNA, including small RNAs such as miRNA, small interfering RNA (siRNA), and small nuclear RNA (snRNA), from tissues and cells.
TaqMan Advanced miRNA cDNA Synthesis Kit
The TaqMan Advanced miRNA cDNA Synthesis Kit is used for preparation of cDNA templates from the entire pool of mature miRNAs in a sample.
miR-Amp Universal Amplification
miR-Amp uses universal primers and master mix to uniformly increase the amount of cDNA for each target. miR-Amp reagents are included in the TaqMan Advanced miRNA cDNA Synthesis Kit.
Applied Biosystems real-time PCR instruments
With Applied Biosystems real-time PCR platforms, you get true value with excellent performance, reliability, and world-class support. Designed for compatibility with Applied Biosystems TaqMan Assays and the flexibility of unlimited real-time chemistry choices, we make it easy for you to get started with your experiments.
Additional information
| Species | TaqMan Advanced miRNA assays are available for human, mouse, and rat miRNAs. We expect to increase the number of miRNA assays in alignment with the Sanger miRBase Registry. | ||||||
| Assay Components | TaqMan Advanced miRNA assays are delivered in a single tube containing the specific pre-formulated TaqMan Assay (TaqMan MGB probe, and forward and reverse primers). Note: TaqMan Advanced miRNA cDNA Synthesis Kit (Cat. No. A28007) and TaqManFast Advanced Master Mix (Cat. No. 4444556) are recommended for use with TaqMan Advanced miRNA assays. | ||||||
| Available Sizes |
| ||||||
| Shipping and Storage | Shipped at ambient temperature. |
TaqMan Assay documentation files can be downloaded at thermofisher.com/taqmanfiles
Product bulletin: TaqMan Advanced miRNA Assays
White paper: TaqMan Advanced miRNA Assays – superior performance
Your Innovative Research: Detection of miRNAs in nipple aspiration fluid
Application note: TaqMan Advanced miRNA Assays
User guide: TaqMan Advanced miRNA Assays
Quick reference: TaqMan Advanced miRNA Assays
White Paper: A technical guide to identifying miRNA normalizers using TaqMan Advanced miRNA Assays
Reference Guide: miRNA from serum and plasma samples
Application Note: Simultaneous detection of miRNA and mRNA on TaqMan Array Cards using the TaqMan Advanced miRNA workflow
Product Categories
Related Applications
Data Analysis Tools
| Species | TaqMan Advanced miRNA assays are available for human, mouse, and rat miRNAs. We expect to increase the number of miRNA assays in alignment with the Sanger miRBase Registry. | ||||||
| Assay Components | TaqMan Advanced miRNA assays are delivered in a single tube containing the specific pre-formulated TaqMan Assay (TaqMan MGB probe, and forward and reverse primers). Note: TaqMan Advanced miRNA cDNA Synthesis Kit (Cat. No. A28007) and TaqManFast Advanced Master Mix (Cat. No. 4444556) are recommended for use with TaqMan Advanced miRNA assays. | ||||||
| Available Sizes |
| ||||||
| Shipping and Storage | Shipped at ambient temperature. |
TaqMan Assay documentation files can be downloaded at thermofisher.com/taqmanfiles
Product bulletin: TaqMan Advanced miRNA Assays
White paper: TaqMan Advanced miRNA Assays – superior performance
Your Innovative Research: Detection of miRNAs in nipple aspiration fluid
Application note: TaqMan Advanced miRNA Assays
User guide: TaqMan Advanced miRNA Assays
Quick reference: TaqMan Advanced miRNA Assays
White Paper: A technical guide to identifying miRNA normalizers using TaqMan Advanced miRNA Assays
Reference Guide: miRNA from serum and plasma samples
Application Note: Simultaneous detection of miRNA and mRNA on TaqMan Array Cards using the TaqMan Advanced miRNA workflow
Product Categories
Related Applications
Data Analysis Tools
Resources
For Research Use Only. Not for use in diagnostic procedures.