Vanquish Trap Heart-Cut 2D-LC Systems
Achieve in-depth analysis for greater confidence in peak purity and identity with the Thermo Scientific Vanquish Trap Heart-Cut 2D-LC System. This two-dimensional LC (LC-LC) system enables you to collect a single fraction of the first-dimension separation onto a trap column to pre-concentrate, and/or achieve mobile phase compatibility for transferring the fraction for a second-dimension separation or compatibility between UV and MS detection. Heart-cut 2D-LC can reveal hidden impurities that co-elute with the analytes of interest by applying two complementary column chemistries to gain more separation power. The system is configured using modules from the Vanquish Flex UHPLC or Horizon UHPLC systems, delivering performance, robustness and ease of use of the Vanquish LC platform.
Highlights
Greater confidence
Collect single or multiple fractions of the first-dimension separation onto a trap column and transfer the cut-out fraction(s) to a second-dimension column with complementary column chemistry in an automated workflow for more confidence in peak purity.
Increased sensitivity
The trap column enables enrichment of low-abundant analytes and removal of diluting or disturbing solvents from the eluent by trapping and pre-concentrating the analytes of interest to increase sensitivity.
Simplified method development
The second-dimension eluent provides the flow for solvent modulation, such as eluent strength reduction or buffer exchange of the first-dimension fraction. This process can address solvent incompatibility of UV and MS methods or mobile phase incompatibility between different column chemistries to support multiple combinations of 1D and 2D methods.
Save time and resources
Automated workflows, with predefined trap and elution steps, allow for unattended sample analysis while reducing human error, saving time and bench space compared to offline approaches.
Flexibility
The modularity of the Vanquish LC systems makes it easy to build a system to best meet your application requirements with flexibility to subsequently upgrade a standard HPLC instrument to a more advanced 2D-LC configuration for more complex analyses.
Easy and reliable tool-free connections
The Vanquish Trap Heart-Cut 2D-LC system is fitted with Thermo Scientific Viper fingertight fittings, which support near-zero dead volume connections and enable sharper peaks and tool-free setup.
What is trap-based heart-cut 2D-LC?
Our trap-based heart-cut 2D-LC system allows you to automate and cut out a single fraction eluting from the first dimension (1D) column onto a trap column. This allows for pre-concentrating low-abundant analytes and addresses solvent incompatibility issues before the fraction is eluted onto a second dimension (2D) column to resolve difficult or co-eluting peaks.
The flow scheme illustrates how the two six-port valves in combination with the 2D pump and the t-piece are used to reduce the solvent strength and to load the cut-out 1D fraction onto the trap column pre-concentrating the sample before being loaded on the second dimension (2D) column.
Flow scheme of the different steps in single heart-cut trapping on the trap column with subsequent solvent modulation (green fluidic pathway), washing, and analysis on the 2D column.
Videos
System specifications
The table below provides an overview of the Vanquish Trap Heart-Cut 2D-LC system specifications for standard setups. Not all specifications are listed. Specifications may differ based on the specific system components selected. See detailed Component Specifications for more information.
| Category | Vanquish Trap Heart-Cut 2D-LC System |
| System pressure, maximum | 1000 or 1500 bar, depends on system configuration |
| Flow range | 0–5 or 0–8 mL/min, depends on pumps selected |
| Pump options (default gradient delay volume) | Binary (35, 200 µL) Delay volume depends on pump(s) selected |
| Biocompatible | Yes |
| pH range | 2–12, chloride concentration up to 1 mol/L |
| System design | Modular |
| Touchscreen user interface display compatible (optional) | Yes, depends on system configuration |
| Solvent monitoring system compatible (optional) | Yes, depends on system configuration |
| Customizable gradient delay volume to simplify method transfer | Yes, with metering device |
| SmartInject technology for maximum injection precision and improved column lifetime | Yes |
| Injection volume range | Standard 0.01-25 µL Optional: 0.01-100 µL |
| Sample cooling | Yes, 4-40°C |
Sample capacity (standard) Based on standard 12 mm OD vials (≤ 1.5 mL) |
4 racks of 54 vials (≤ 1.5 mL) each, other rack formats available + capacity of 12 × 22.5 mm OD vials (≤ 10 mL) in the carousel |
| Sampler extension available (optional) | Yes, with Vanquish Charger |
| Column compartment thermostatting | Yes, 5-120 °C |
Column compartment pre-heater |
Active and passive |
| Column compartment post-column cooler | Yes |
| Compatible Detectors | Vanquish Charged Aerosol Detector Vanquish Diode Array Detector Vanquish Fluorescence Detector Vanquish Multiple Wavelength Detector Vanquish Variable Wavelength Detector Refractive Index Detector Mass Spectrometer |
Component specifications
Standard Vanquish Trap Heart-Cut 2D-LC systems are made up of the following components. Download the associated specifications sheet for more detail on each component.
| Vanquish Horizon Trap Heart-Cut 2D-LC System | Vanquish Flex Trap Heart-Cut 2D-LC System | |
Pump
|
2 Vanquish Binary Pump H
Note: Binary pump recommended as ²D pump |
2 Vanquish Binary Pump F Note: Binary pump recommended as ²D pump |
Autosampler
|
Vanquish Split Sampler HT | Vanquish Split Sampler FT |
Extended sample capacity (optional)
|
Vanquish Charger (optional) |
|
Column compartment
|
Vanquish Column Compartment H | |
Virtually zero dead volume system connections
|
Viper UHPLC Fittings Kits | |
Detectors
|
Visit our HPLC and UHPLC Detectors page | |
For Research Use Only. Not for use in diagnostic procedures.