High-Throughput, High Resolution, Data-Independent Acquisition Workflow for Accurate Label-Free Quantitation

Get the details on the qualitative and quantitative performance of the Thermo Scientific Velocity LFQ DIA quantitative proteomics platform, featuring label-free quantitation (LFQ) with an optimized data-independent acquisition (DIA) method. It offers uniquely accurate, precise quantitation with deep proteome coverage and data completeness for high-confidence characterization of the differences between biological systems. This technical note presents a robust and reproducible workflow for accurately quantifying and identifying hundreds to thousands of proteins from complex sample mixtures with a high background of human peptides.

The experiments demonstrate that the Velocity LFQ DIA platform offers:

• Excellent quantitation accuracy and precision for small amounts of protein from challenging sample matrices
• High sample throughput and data quality while also achieving high proteome coverage
• Long-term stability and reproducibility across the whole workflow

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