Atomic-resolution cryo-EM is finally here. Structural biologists used a Thermo Scientific Krios G4 Cryo-TEM equipped with a Thermo Scientific Selectris X Imaging Filter to achieve record-breaking resolution results, recently featured on the cover of Nature and other recent articles from Science and Nature . Researchers from the Medical Research Council Laboratory of Molecular Biology in Cambridge, UK, obtained a 1.2 ångström resolution structure of the iron-storing protein apoferritin. They also achieved a 1.7 ångström resolution of membrane protein (GABA receptor), with an even sharper resolution in key parts of the protein. This human membrane protein has target sites for general anesthetics, benzodiazepines, barbiturates and neuroactive steroids, making it very relevant to structure-based drug discovery. In addition to the resolution leap, the Selectris technology will also significantly impact productivity. By having a better filter and camera, scientists will be able to achieve a specific resolution with much less data.
Selectris and Selectris X Imaging Filters are fully compatible with the Thermo Scientific Glacios Cryo-TEM, featuring 200 kV XFEG optics. The Glacios Cryo-TEM bundles all this into a small footprint that simplifies installation. See more of what's possible on the Glacios Cryo-TEM with Selectris X Imaging Filters.
Find out more about atomic-resolution cryo-EM by viewing on-demand replays of our online launch series. You’ll see the most recent results from the Selectris Imaging Filter.
Cryo-electron microscopy: Membrane proteins down to atoms
April 29, 2021
See how our 300 kV Krios G4 Cryo-TEM works from the cold field emission source to the detector and Selectris Imaging Filter. We will also show brand new results of membrane protein structures going beyond 2.5 Å resolution obtained with our 200 kV Glacios Cryo-TEM.
Speakers:
Stephen Brohawn, Assistant Professor, Department of Molecular & Cell Biology Helen Wills Neuroscience Institute, University of California, Berkeley
Abhay Kotecha, Senior Applications Development Scientist, Thermo Fisher Scientific
Marc Storms, Product Marketing Manager, Thermo Fisher Scientific
Atomic Resolution Cryo-EM: There’s More!
November 5, 2020
Structural biologists used a Thermo Scientific Krios G4 Cryo-TEM equipped with a Thermo Scientific Selectris Imaging Filter to achieve record-breaking resolution results, which were published in bioRxiv in May, published and recently featured on the cover of Nature .
But there’s more to the story.
Find out what’s next for atomic resolution cryo-EM at our livestream event Atomic Resolution Cryo-EM: There’s More! You'll get a front row seat to see the record-breaking resolution results.
Abhay Kotecha, Senior Applications Development Scientist, Thermo Fisher Scientific
Abhay received his PhD in Structural Biology at the University of Oxford, UK. He spent further time at Oxford as a post-doc continuing to a position as Senior Research Scientist. Abhay has been with Thermo Fisher Scientific for over two years and an expert in single particle analysis, MicroED and cryo-electron tomography.
Marc Storms, Product Marketing Manager, Thermo Fisher Scientific
Marc is part of the Life Sciences Business Unit in Thermo Fisher’s Materials and Structural Analysis Division. He has been involved in the development and launch of various cryo-EM products and workflow solutions such as the Titan Krios, Glacios, Vitrobot, single particle analysis software (EPU), phase plates and various generations of the Falcon direct electron detector.
Gijs Van Duinen, Cryo-EM Product Manager, Thermo Fisher Scientific
Since 2018, Gijs leads the cryo-TEM product management team in the Life Sciences Business Unit in Thermo Fisher’s Materials and Structural Analysis Division. He joined the company in 2007, having managed the cryo-EM facility a Imperial College, London, and held various roles in customer applications support and R&D.
Cryo-EM: What’s Next
August 10, 2020
Cryo-electron microscopy (cryo-EM) represents the next frontier in structural biology. This revolutionary technique can now resolve protein structures to true atomic resolution with the new Selectris and Selectris X Imaging Filters.
View product announcement with either English or Chinese subtitles.
Speakers:
Trisha Rice, VP and GM Life Sciences Business, Materials & Structural Analysis
Steve Reyntjens, Director Product Marketing, Life Sciences, Materials & Structural Analysis
Cryo-EM: What’s Next
August 11, 2020
Get an unprecedented behind-the-scenes look at innovation in action on the manufacturing floor in Eindhoven, Netherlands. Learn about the R&D team and technology behind the Selectris and Selectris X Imaging Filters. Listen first-hand how the team through ingenuity and rigorous testing achieved unprecedented stability and automation for the end-user.
Speakers:
Jamie Mc Cormack, Sr Staff Engineer, R&D
Gijs Van Duinen, Product Manager, Cryo-EM
Cryo-EM: What’s Next
August 13, 2020
Discover what you can do with atomic-resolution cryo-EM through the Selectris Imaging Filters and latest Falcon 4 camera. See the record-breaking structure results for single particle and cellular cryo-tomography from MRC-LMB, MPI Biochemistry, IST Austria, and others. Learn the potential applications of the Selectris for small proteins (<100kDa) because of improved imaging contrast through zero-loss filtering. High resolution also helped achieve better model building leading to corrected chemistries.
Speaker:
Abhay Kotecha, Sr Applications Development Scientist
