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The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated human NFkB p65 in cell lysates. Detection of mouse and rat NFkB is expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.
Nuclear factor kB p65 (NF-kB p65) is encoded by the RELA gene and is present on chromosome 11 in humans. NF-kB P65 is also known as RelA (v-rel avian reticuloendotheliosis viral oncogene homolog A) and belongs to the Rel family of proteins. It is one of the two subunits of NF-kB (Nuclear factor kappa-light-chain-enhancer of activated B cells) that heterodimerizes with the other subunits p50 or p52. However, binding of TNF alpha to its cognate receptor phosphorylates IKK which in turn phosphorylates I kB allowing proteasomal degradation of I kB. It specifically plays a key role in transcription of immunoglobulin k (kappa) gene in mature B-lymphoid cells.
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Gene aliases : NFKB3, p65, RELA
Gene ID : (Human) 5970
Gene symbol : RELA
Protein Aliases : NF-kappa-B p65delta3, NF-kappa-B transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, Transcription factor p65, v-rel avian reticuloendotheliosis viral oncogene homolog A, v-rel reticuloendotheliosis viral oncogene homolog A
UniProt ID (Human) Q04206
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