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Analytical sensitivity
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Homogenous (no wash)
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Detector antibody conjugate
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About This Kit
900-K398K consists of: 900-K398 (capture antibody, detection antibody, and protein standard), and AVIDIN-60UL (avidin HRP-conjugate).
Human IL-33 ABTS ELISA Development Kit contains the key components required for the quantitative measurement of natural and/or recombinant human IL-33 in a sandwich ELISA format. Human IL-33 ABTS ELISA Development Kit contains enough material to assay the target cytokine in at least 1,000 ELISA plate wells.
The ABTS ELISA Buffer Kit (Product # 900-K00), sold separately, is recommended to be used in conjunction with this ABTS ELISA Development Kit for optimal performance. This buffer kit contains all the necessary components to assay ten 96-well ELISA plates (included) and contains detailed handling instructions.
IL-33 (Interleukin-33) is a 270 amino acid, highly divergent protein belonging to the IL-1 family with an IL-1-like C-terminal domain. IL-33 is a dual function protein that may function both as a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. IL-33 binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6. IL-33 activates NF-kappaB and MAP kinases, and drives production of TH2-associated cytokines from in vitro polarized TH2 cells. In vivo, IL-33 induces the expression of IL-4, IL-5, and IL-13 and leads to severe pathological changes in mucosal organs. IL-33 is proteolytically converted to a mature form by CASP1 and is highly expressed in high endothelial venules found in tonsils, Peyer's patches and mesenteric lymph nodes and is almost undetectable in placenta. Prolonged IL-33 treatment of mice led to the development of eosinophilia, splenomegaly, and severe pathological changes in mucosal organs such as lungs, esophagus and small intestine. Recent experiments have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. Despite its predicted molecular weight, IL-33 will often run at higher molecular weight in SDS-PAGE. Studies have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine, and an intracellular nuclear factor with transcriptional regulatory properties.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Bioinformatics
Gene aliases : C9orf26, DER4, DVS27, FIT-1, IL1F11, IL1RL1, IL33, IL33R, NF-HEV, NFEHEV, NFHEV, ST2, ST2L, ST2V, T1
Gene ID : (Human) 9173, (Human) 90865
Gene symbol : IL1RL1, IL33
Protein Aliases : DVS27-related protein, growth stimulation-expressed, homolog of mouse growth stimulation-expressed, IL-1F11, IL-33, interleukin 1 receptor-like 1, interleukin 1 receptor-related protein, Interleukin-1 family member 11, Interleukin-1 receptor-like 1, Interleukin-33, NF-HEV, nuclear factor for high endothelial venules, Nuclear factor from high endothelial venules, Protein ST2
UniProt ID (Human) Q01638, (Human) O95760
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