Traditional fluorescence imaging of oxidative stress and apoptosis using CellROX® Deep Red Reagent (Cat. No. C10422) and CellEvent™ Caspase-3/7 (Cat. No. C10423)

HeLa cells were treated with or without 0.5 µM staurosporine for 2 or 4 hours in the presence of 7.5 µM fluorogenic caspase 3/7 substrate. Cells were then stained with 5 µM Far Red ROS Sensor and Hoechst 33342 for 30 minutes at 37°C, then washed with warm DPBS. Cells were imaged immediately on a Zeiss Axiovert® inverted microscope using a 40x objective. Increased oxidative stress was observed at 2 hours after treatment (magenta) while caspase 3/7 activation was not observed until 4 hours after treatment (green) as shown in representative cells above.

HeLa cells were treated with or without 0.5 µM staurosporine for 2 or 4 hours in the presence of 7.5 µM fluorogenic caspase 3/7 substrate. Cells were then stained with 5 µM Far Red ROS Sensor and Hoechst 33342 for 30 minutes at 37°C, then washed with warm DPBS. Cells were imaged immediately on a Zeiss Axiovert® inverted microscope using a 40x objective. Increased oxidative stress was observed at 2 hours after treatment (magenta) while caspase 3/7 activation was not observed until 4 hours after treatment (green) as shown in representative cells above.

Related Products

Related Images

REF-52 fibroblasts. Cyclic AMP Fluorosensor (FlCRhR) and fura-2 AM Go ›

BPAE cells fixed and permeabilized using the Image-iT® Fixation/Permeabilization Kit. Go ›

Oxidative stress analysis in BPAE and Raw cells using CellROX® Deep Red Reagent (Cat. No. C10422) Go ›

BPAE detection of oxidative stress using MitoTracker® Green (Cat. No. M7514) and CellROX® Deep Red Reagent (Cat. No. C10422) Go ›

Agarose gel containing camptothecin-treated HL-60 cells. Go ›

Imaging autophagy in live HeLa cells with CellLight® reagents for mitochondria and lysosomes: Go ›

Live cell imaging with CellLight™ reagents. Go ›

Live cells transduced with Organelle Lights™ or Cellular Lights™ reagents. Go ›

CD335 (NKp46) Antibody (63335182) in RE Go ›

CD223 (LAG-3) Antibody (56223942) in TM Go ›