This protocol library includes ready-made software protocols that can be used to run commercially available or commonly well-known assays with the Thermo Scientific™ Multiskan™ GO Microplate Spectrophotometer. The files contain protocols for the Thermo Scientific™ SkanIt™ Software version 3.2, which is the PC software used to control the instrument and to perform calculations to measurement data. The instrument can also be used without a PC, but these protocols are compatible only with SkanIt Software.
The protocols can be downloaded from this website and imported to the database of the SkanIt Software for Multiskan GO. The software protocols are instrument-specific, therefore, they cannot be imported to the software of any other instrument. The protocol files cannot be viewed outside the SkanIt Software.
All the protocols are made for 96-well microplate format.
The protocols have been made according to the available kit instructions. The user performing the assay has the ultimate responsibility to verify that the definitions, parameters, and calculations of the protocol match the kit instructions and the assay requirements. Thermo Fisher Scientific will not assume any responsibility for the results of the assays performed with these software protocols.
Read first the instructions on downloading the SkanIt Software protocols of the Multiskan GO.
Note: SkanIt software sessions on the protocol library are compatible with the SkanIt version 3.2 software for Multiskan GO only. Sessions for the 4th generation SkanIt software are under development and will be available soon.
| Assay | Description | SkanIt Software Protocol Download |
| dsDNA quantitation | dsDNA quantitation and 260/280 ratio calculation |  |
| Promega CellTiter 96 AQueous One Solution Cell Proliferation Assay | Determining the number of viable cells in proliferation or cytotoxicity assays | |
| Thermo Scientific Coomassie (Bradford) Protein Assay | Total protein quantitation | |
| Thermo Scientific Coomassie Plus (Bradford) Protein Assay | Total protein quantitation | |
| Thermo Scientific 660 nm Protein Assay | Total protein quantitation | |
| Thermo Scientific BCA Protein Assay | Total protein quantitation | |
| Thermo Scientific Modified Lowry Protein Assay | Total protein quantitation | |
| Thermo Scientific Human TNF Alpha ELISA | Quantification of human Tumor Necrosis Factor alpha | |
| Thermo Scientific Human IFN gamma ELISA | Quantification of human Interferon gamma | |
| Thermo Scientific Mouse IFN gamma ELISA | Quantification of mouse Interferon gamma | |
| Thermo Scientific Human IL-8 ELISA | Quantification of human Interleukin-8 | |
| Thermo Scientific Human IL-1 beta ELISA | Quantification of human Interleukin-1 beta | |
| Thermo Scientific Human IL-2 ELISA | Quantification of human Interleukin-2 | |
| Thermo Scientific Human IL-6 ELISA | Quantification of human Interleukin-6 | |
| Thermo Scientific Mouse TNF alpha ELISA | Quantification of mouse Tumor Necrosis Factor alpha | |