Example
What percent of the product molecules contain an error after PCR (30 cycles) with different polymerases?
Polymerase | 1 kb template | 3 kb template |
|---|---|---|
Phusion High-Fidelity DNA Polymerases (HF Buffer) | 1.32% | 3.96% |
Phusion High-Fidelity DNA Polymerases (GC Buffer) | 2.85% | 8.55% |
Pyrococcus furiosus DNA polymerase | 8.4% | 25.2% |
Taq DNA polymerase | 68.4% | 205.2% |
The table above demonstrates the low error rate of Phusion DNA Polymerase. After 30 cycles of PCR amplifying a 3 kb template, only 3.96 % of the product DNA molecules contain 1 (nucleotide) error each. This means that 96.04 % of the product molecules are entirely error-free. In contrast, after the same PCR protocol performed with Taq DNA polymerase, every product molecule contains an average of 2 errors.