Protein-Protein Interactions Support—Troubleshooting

• The tagged protein may have been degraded. Make sure that you included protease inhibitors in your lysis buffer.
• Confirm that the fusion protein was properly cloned into the expression vector.
• Use more lysate for the pulldown.
• Use a more sensitive detection system such as SuperSignal™ West Femto Maximum Sensitivity Substrate (Cat. No.34095).

Here are possible causes and solutions:

Here are possible causes and solutions:

Here are possible causes and solutions:

Here are possible causes and solutions:

Here are possible causes and solutions:

Here are possible causes and solutions:

Here are possible causes and solutions:

Here are possible causes and solutions:

Here are possible causes and solutions:

The protein-protein interaction may be transient in nature. Often times, adding crosslinkers to capture putative interacting partners inside or outside of the cell may maintain the interaction through lysis and enrichment/purification. Membrane permeable crosslinkers like DSS (Cat. No. 21658) allow one to “freeze” interactions inside the cell, whereas membrane impermeable crosslinkers like BS3 (Cat. No. 21585) only work on the cell surface or outside the cell.

• Choosemore sophisticated crosslinkers with photo-reactive groups, which can be made to react at selected times and only in response to irradiation by UV light. Heterobifunctional crosslinkers with a thermoreactive group (spontaneously reactive) at one end and a photo-reactive group on the other end can be reacted first through the thermoreactive end with a protein that can be used as bait for other interacting proteins. The modified protein is introduced into a sample and allowed to interact with other proteins. Then the sample is exposed to UV light, which causes the photo-reactive end of the modified protein to covalently link to nearby molecules, thus “freezing” in place any interacting protein as a complex.
• Remove non-reacted reagent by dialysis or desalting

• Check to see whether there were any substances in the buffer that would prevent conjugation to the crosslinker. For example, primary amine containing buffers like Tris or glycine would out-compete amine-reactive crosslinkers like DSS or BS3. Sodium azide with a concentration over 0.02% is equally problematic.
• Confirm that you used a membrane-permeable crosslinker for intracellular crosslinking.
• Ensure that the pH was proper for the crosslinking to occur.
• Use fresh crosslinkers.
• For a photo-reactive crosslinker, check that the sample was exposed to the proper UV wavelength (300-370 nm) at the proper distance and exposure time.

1. Determine the label incorporation of the bait protein before photoactivation with the prey protein, by western blot analysis.
2. Check the UV light source to ensure good photoactivation (see Photolysis Information section of the Sulfo-SBED manual for guidelines).
3. If the target (prey) protein concentration is too low, enrich the protein system if possible.
4. Optimize the antibody concentrations used in western blot.
5. It is possible that the protein interaction is blocking the epitope, which may occur with monoclonal antibodies and peptide-directed antibodies; in this case, use an antibody from a different source or probe with Streptavidin-HRP.
6. Milk contains endogenous biotin and its use as a blocking buffer during the detection assay could mask the bands of the biotin-labeled interaction partner.

There are several approaches to eliminate excess bands on a blot. These include the following:

1. Try a different blocking agent (keep in mind that milk contains high amount of biotin and could mask any specific results).
2. The target sample may contain other proteins which bind to the labeled ligand. Add Tween™-20 (0.005%, v/v) to the 1X Label Transfer Buffer. Alternatively, increase the stringency of the washes by increasing the concentration of detergent from 0.05% Tween-20 to 0.1%, increase the salt content of the wash buffer, or adjust the pH of the buffer to see if any of these steps will release the non-covalently bound proteins to the target protein.
3. Enrich the complex sample using immobilized monomeric avidin.
4. It is possible that too much protein was loaded on the electrophoresis gel; in this case, apply a smaller amount of protein on the gel.
5. Reduce the amount of sulfo-SBED biotin used to label the bait protein as the crosslinking process could be covalently attaching the bait protein to non-relevant proximal proteins.

Confirm that the reducing agent is fully cleaving the bait protein off of the target protein. Increase the concentration of the reducing agent or use fresh reducing agent if the bait:target protein is not being cleaved apart. 

The following are the reasons and remedies for low signal in far-western blot application:

1. Streptavidin-HRP is too little or has low activity: Try 2- to 5-fold higher concentration or use fresh streptavidin-HRP.
2. Biotinylated-protein probe is too dilute: Try 2- to 5-fold higher biotinylated-protein probe concentration. 
   Note: Alter the biotinylated “probe” (bait) protein concentration only if adjusting the Streptavidin-HRP concentration has no effect or results in high background. 
3. Probe protein not sufficiently biotinylated: Biotinylate probe (bait) protein again with a higher molar excess of the biotinylation reagent to the protein concentration; mole-to-mole ratio of biotin may be determined using HABA (Cat. No. 28010) or Fluorescence Biotin Quantitation Kit (Cat. No. 46610) each time the probe is biotinylated to make sure that consistent biotinylation level is obtained.
   Note: There can be a fine line between sufficient labeling for detection and over labeling with subsequent steric hindrance and loss of signal. Optimizing the labeling reaction prior to the far-Western can reduce downstream optimization, which can use up precious samples.
4. Biotinylated protein probe interacts weakly with “prey” protein: Use native conditions to separate samples, as denaturing conditions may alter the proteins such that they do not interact with the “probe” during electrophoresis and transfer.
5. It is possible that a change in three-dimensional structure took place due to the protein absorbing into the membrane, changing the interaction site. In this case, far-western analysis may not be an appropriate approach for detection of a protein-protein interaction and cross-linking or label transfer may be other viable options to confirm the interacting partnership. 

There are several approaches to eliminate excess bands on a blot. These include the following:

• Use a compatible blocking buffer to dilute the biotinylated-protein probe and Streptavidin-HRP (avoid milk which contains endogenous biotin and can interfere with signal production).
• Target sample may contain other proteins which bind to labeled ligand. Add Tween™-20 (0.005%, v/v) to the 1X Label Transfer Buffer. Alternatively, increase the stringency of the washes by increasing the concentration of detergent from 0.05% Tween-20 to 0.1%, increase the salt content of the wash buffer, or adjust the pH of the buffer to see if any of these steps will release the non-covalently bound proteins to the target protein.
• Enrich complex sample using immobilized monomeric avidin.
• It is possible that too much protein was loaded on the electrophoresis gel; in this case, apply a smaller amount of protein on the gel.