LentiArray and LentiPool CRISPR Libraries

The Invitrogen LentiArray CRISPR Human Druggable Genome Library is ideal for identifying potentially therapeutics targets involved in the development and progression of disease. The gene targets within the LentiArray CRISPR Human Druggable Genome Library were selected using the most up-to-date genome databases, including the NCBI RefSeq database, and cross-referenced to the Gene Ontology Consortium (GO) database and/or the HUGO Gene Nomenclature Committee (HGNC). The gRNA designs for each target were then created using a proprietary design algorithm developed by the scientists at Thermo Fisher Scientific. The LentiArray CRISPR Human Druggable Genome Library targets 10,132 genes with up to four gRNAs per gene target (pooled in a single well), for a total of 40,512 gRNAs. Libraries are delivered as 100 μL of ready-to-use lentiviral particles per gene target with an average titer of 1X10⁸ TU/ml and are also available as glycerol stocks. Libraries are supplied in 96-well plates.

The Invitrogen LentiPool CRISPR library has the same gene coverage and is supplied as lentiviral pools containing four gRNAs per gene, delivered as ready-to-use lentivirus at high titer: >1 x 10⁸ TU/mL.

• Ready to use
• Glycerol stock
• LentiPool format

Kinases are involved in the majority of signaling cascades, and dysregulation of kinase activity is linked to development of numerous diseases, making kinases a key class of drug targets. The gene targets within the Invitrogen LentiArray CRISPR Human Kinase Library were selected using the most up-to-date genome databases, including the NCBI RefSeq database, and cross-referenced to the Gene Ontology Consortium (GO) database and/or the HUGO Gene Nomenclature Committee (HGNC) as well as additional resources such as the KinBase database. The gRNA designs for each target were then created using a proprietary design algorithm developed by the scientists at Thermo Fisher Scientific. The LentiArray CRISPR Human Kinase Library targets 822 genes with up to four gRNAs per gene target (pooled in a single well), for a total of 3,288 gRNAs. Libraries are delivered as 100 μL of ready-to-use lentiviral particles per gene target with an average titer of 1X10⁸ TU/ml and are also available as glycerol stocks. Libraries are supplied in 96-well plates.

The Invitrogen LentiPool CRISPR library has the same gene coverage and is supplied as lentiviral pools containing four gRNAs per gene, delivered as ready-to-use lentivirus at high titer: >1 x 10⁸ TU/mL.

• Ready to use
• Glycerol stock
• LentiPool format

G protein–coupled receptors (GPCRs) are regulators of a wide range of physiological and disease processes. GPCRs have emerged as one of the most druggable classes of genes, with nearly 30% of FDA-approved drugs targeting GPCRs. The gene targets within the Invitrogen LentiArray CRISPR Human GPCR Library were selected using the most up-to-date genome databases, including the NCBI RefSeq database, and cross-referenced to the Gene Ontology Consortium (GO) database and/or the HUGO Gene Nomenclature Committee (HGNC). The gRNA designs for each target were then created using a proprietary design algorithm developed by the scientists at Thermo Fisher Scientific. The LentiArray CRISPR Human GPCR Library targets 446 genes with up to four gRNAs per gene target (pooled in a single well), for a total of 1,784 gRNAs. Libraries are delivered as 100 μL of ready-to-use lentiviral particles per gene target with an average titer of 1X10⁸ TU/ml and are also available as glycerol stocks. Libraries are supplied in 96-well plates.

The Invitrogen LentiPool CRISPR library has the same gene coverage and is supplied as lentiviral pools containing four gRNAs per gene, delivered as ready-to-use lentivirus at high titer: >1 x 10⁸ TU/mL.

• Ready to use
• Glycerol stock
• LentiPool format

The Invitrogen LentiArray CRISPR Human Cancer Biology Library is a collection that targets some of the most common genes involved in the development of cancer. The gene targets within the LentiArray CRISPR Human Cancer Biology Library were selected using the most up-to-date genome databases, including the NCBI RefSeq database, and cross-referenced to the Gene Ontology Consortium (GO) database and/or the HUGO Gene Nomenclature Committee (HGNC) as well as The Cancer Genome Atlas (TCGA). The gRNA designs for each target were then created using a proprietary design algorithm developed by the scientists at Thermo Fisher Scientific. The LentiArray CRISPR Human Cancer Biology Library targets 510 genes with up to four gRNAs per gene target (pooled in a single well), for a total of 2,040 gRNAs. Libraries are delivered as 100 μL of ready-to-use lentiviral particles per gene target with an average titer of 1X10⁸ TU/ml and are also available as glycerol stocks. Libraries are supplied in 96-well plates.

The Invitrogen LentiPool CRISPR library has the same gene coverage and is supplied as lentiviral pools containing four gRNAs per gene, delivered as ready-to-use lentivirus at high titer: >1 x 10⁸ TU/mL.

• Ready to use
• Glycerol stock
• LentiPool format

Epigenetic regulation of gene expression plays a central role in normal development and is being recognized as a contributing factor to the development of many diseases. The gene targets within the Invitrogen LentiArray CRISPR Human Epigenetics Library were selected using the most up-to-date genome databases, including the NCBI RefSeq database, and cross-referenced to the Gene Ontology Consortium (GO) database and/or the HUGO Gene Nomenclature Committee (HGNC). The gRNA designs for each target were then created using a proprietary gRNA design algorithm developed by the scientists at Thermo Fisher Scientific. The LentiArray CRISPR Human Epigenetics Library targets 396 genes with up to four gRNAs per gene target (pooled in a single well), for a total of 1,548 gRNAs. Libraries are delivered as 100 μL of ready-to-use lentiviral particles per gene target with an average titer of 1X10⁸ TU/ml and are also available as glycerol stocks. Libraries are supplied in 96-well plates.

The Invitrogen LentiPool CRISPR library has the same gene coverage and is supplied as lentiviral pools containing four gRNAs per gene, delivered as ready-to-use lentivirus at high titer: >1 x 10⁸ TU/mL.

• Ready to use
• Glycerol stock
• LentiPool format

The ubiquitin system is integral to maintaining cellular homeostasis by regulating protein turnover. Dysregulation of the ubiquitin system has been linked to multiple diseases including cancer and neurodegenerative, musculoskeletal, cardiovascular, and metabolic diseases, as well as being linked to viral infection. The gene targets within the Invitrogen™ LentiArray™ CRISPR Human Ubiquitin Library were selected using the most up-to-date genome databases, including the NCBI RefSeq database, and cross-referenced to the Gene Ontology Consortium (GO) database and/or the HUGO Gene Nomenclature Committee (HGNC). The gRNA designs for each target were then created using a proprietary gRNA design algorithm developed by the scientists at Thermo Fisher Scientific. The LentiArray CRISPR Human Ubiquitin Library targets 943 genes with up to four gRNAs per gene target (pooled in a single well), for a total of 3,722 gRNAs. Libraries are delivered as 100 μL of ready-to-use lentiviral particles per gene target with an average titer of 1X10⁸ TU/ml and are also available as glycerol stocks. Libraries are supplied in 96-well plates.

The Invitrogen LentiPool CRISPR library has the same gene coverage and is supplied as lentiviral pools containing four gRNAs per gene, delivered as ready-to-use lentivirus at high titer: >1 x 10⁸ TU/mL.

• Ready to use
• Glycerol stock
• LentiPool format

Cell cycle regulators are important to normal development and also play a role in the development of cancer and cardiovascular, inflammatory, and neurodegenerative diseases. The gene targets within the Invitrogen LentiArray CRISPR Human Cell Cycle Library were selected using the most up-to-date genome databases, including the NCBI RefSeq database, and cross-referenced to the Gene Ontology Consortium (GO) database and/or the HUGO Gene Nomenclature Committee (HGNC). The targets include cyclin-dependent kinases, or CDKs, which are crucial for cell cycle progression; regulators of cell cycle progression such as CIP/KIP family proteins and members of the INK4 family of cell cycle inhibitors; proteins in the retinoblastoma family; and DNA replication factors such as the cell division cycle proteins (CDCs). The gRNA designs for each target were then created using a proprietary gRNA design algorithm developed by the scientists at Thermo Fisher Scientific. The LentiArray CRISPR Human Cell Cycle Library targets 1,444 genes with up to four gRNAs per gene target (pooled in a single well), for a total of 5,776 gRNAs. Libraries are delivered as 100 μL of ready-to-use lentiviral particles per gene target with an average titer of 1X10⁸ TU/ml and are also available as glycerol stocks. Libraries are supplied in 96-well plates.

The Invitrogen LentiPool CRISPR library has the same gene coverage and is supplied as lentiviral pools containing four gRNAs per gene, delivered as ready-to-use lentivirus at high titer: >1 x 10⁸ TU/mL.

• Ready to use
• Glycerol stock
• LentiPool format

Membrane trafficking proteins are involved in a number of processes, including neurotransmitter and endocrine release, phagocytosis, endocytosis, and autophagy. The gene targets within the Invitrogen LentiArray CRISPR Human Membrane Trafficking Library were selected using the most up-to-date genome databases, including the NCBI RefSeq database, and cross-referenced to the Gene Ontology Consortium (GO) database and/or the HUGO Gene Nomenclature Committee (HGNC). The gRNA designs for each target were then created using a proprietary gRNA design algorithm developed by the scientists at Thermo Fisher Scientific. The LentiArray CRISPR Human Membrane Trafficking Library targets 141 genes with up to four gRNAs per gene target (pooled in a single well), for a total of 564 gRNAs . Libraries are delivered as 100 μL of ready-to-use lentiviral particles per gene target with an average titer of 1X10⁸ TU/ml and are also available as glycerol stocks. Libraries are supplied in 96-well plates.

The Invitrogen LentiPool CRISPR library has the same gene coverage and is supplied as lentiviral pools containing four gRNAs per gene, delivered as ready-to-use lentivirus at high titer: >1 x 10⁸ TU/mL.

• Ready to use
• Glycerol stock
• LentiPool format

Transcription factors are key regulators of gene expression. The gene targets within the Invitrogen LentiArray CRISPR Human Transcription Factor Library were selected using the most up-to-date genome databases, including the NCBI RefSeq database, and cross-referenced to the Gene Ontology Consortium (GO) database and/or the HUGO Gene Nomenclature Committee (HGNC). The gRNA designs for each target were then created using a proprietary gRNA design algorithm developed by the scientists at Thermo Fisher Scientific. The LentiArray CRISPR Human Transcription Factor Library targets 1,931 genes with up to four gRNAs per gene target (pooled in a single well), for a total of 7,724 gRNAs. Libraries are delivered as 100 μL of ready-to-use lentiviral particles per gene target with an average titer of 1X10⁸ TU/ml and are also available as glycerol stocks. Libraries are supplied in 96-well plates.

The Invitrogen LentiPool CRISPR library has the same gene coverage and is supplied as lentiviral pools containing four gRNAs per gene, delivered as ready-to-use lentivirus at high titer: >1 x 10⁸ TU/mL.

• Ready to use
• Glycerol stock
• LentiPool format

Nuclear hormone receptors are a family of transcription factors that are activated by lipid-soluble ligands such as steroid hormones, thyroid hormone, vitamin D, and retinoic acid. Nuclear hormone receptors regulate a range of biological processes, including metabolism, development, proliferation, and reproduction. The gene targets within the Invitrogen LentiArray CRISPR Human Nuclear Hormone Receptor Library were selected using the most up-to-date genome databases, including the NCBI RefSeq database, and cross-referenced to the Gene Ontology Consortium (GO) database and/or the HUGO Gene Nomenclature Committee (HGNC). The gRNA designs for each target were then created using a proprietary gRNA design algorithm developed by the scientists at Thermo Fisher Scientific. The LentiArray CRISPR Human Nuclear Hormone Receptor Library targets 47 genes with up to four gRNAs per gene target (pooled in a single well), for a total of 188 gRNAs. Libraries are delivered as 100 μL of ready-to-use lentiviral particles per gene target with an average titer of 1X10⁸ TU/ml and are also available as glycerol stocks. Libraries are supplied in 96-well plates.

The Invitrogen LentiPool CRISPR library has the same gene coverage and is supplied as lentiviral pools containing four gRNAs per gene, delivered as ready-to-use lentivirus at high titer: >1 x 10⁸ TU/mL.

• Ready to use
• Glycerol stock
• LentiPool format

Apoptosis is a tightly regulated process that is essential for maintaining homeostasis in multicellular organisms. Inhibition of apoptosis can result in the development of cancer, autoimmune, and inflammatory diseases and is also involved in viral infection. Conversely, overactivation of apoptosis can lead to atrophy, tissue damage, and neurodegenerative disease. The gene targets within the Invitrogen LentiArray CRISPR Human Apoptosis Library were selected using the most up-to-date genome databases, including the NCBI RefSeq database, and cross-referenced to the Gene Ontology Consortium (GO) database and/or the HUGO Gene Nomenclature Committee (HGNC). The gRNA designs for each target were then created using a proprietary gRNA design algorithm developed by the scientists at Thermo Fisher Scientific. The LentiArray CRISPR Human Apoptosis Library targets 904 genes with up to four gRNAs per gene target (pooled in a single well), for a total of 3,616 gRNAs. Libraries are delivered as 100 μL of ready-to-use lentiviral particles per gene target with an average titer of 1X10⁸ TU/ml and are also available as glycerol stocks. Libraries are supplied in 96-well plates.

The Invitrogen LentiPool CRISPR library has the same gene coverage and is supplied as lentiviral pools containing four gRNAs per gene, delivered as ready-to-use lentivirus at high titer: >1 x 10⁸ TU/mL.

• Ready to use
• Glycerol stock
• LentiPool format

Transporter proteins play a key role in pharmacology, affecting how molecules are moved across cellular membranes. The gene targets within the Invitrogen LentiArray CRISPR Human Drug Transport Library were selected using the most up-to-date genome databases, including the NCBI RefSeq database, and cross-referenced to the Gene Ontology Consortium (GO) database and/or the HUGO Gene Nomenclature Committee (HGNC). The gRNA designs for each target were then created using a proprietary gRNA design algorithm developed by the scientists at Thermo Fisher Scientific. The LentiArray CRISPR Human Nuclear Hormone Receptor Library targets 98 genes with up to four gRNAs per gene target (pooled in a single well), for a total of 392 gRNAs. Libraries are delivered as 100 μL of ready-to-use lentiviral particles per gene target with an average titer of 1X10⁸ TU/ml and are also available as glycerol stocks. Libraries are supplied in 96-well plates.

The Invitrogen LentiPool CRISPR library has the same gene coverage and is supplied as lentiviral pools containing four gRNAs per gene, delivered as ready-to-use lentivirus at high titer: >1 x 10⁸ TU/mL.

• Ready to use
• Glycerol stock
• LentiPool format

Ion channels are integral membrane proteins that establish the electrochemical gradient that gives rise to both the resting membrane potential and the formation of action potentials which are crucial to nerve conduction and heart and muscle contraction, as well as other processes such as insulin release from the pancreas and activation of T cells. The gene targets within the Invitrogen LentiArray CRISPR Human Ion Channel Library were selected using the most up-to-date genome databases, including the NCBI RefSeq database, and cross-referenced to the Gene Ontology Consortium (GO) database and/or the HUGO Gene Nomenclature Committee (HGNC). The gRNA designs for each target were then created using a proprietary gRNA design algorithm developed by the scientists at Thermo Fisher Scientific. The LentiArray CRISPR Human Ion Channel Library targets 328 genes with up to four gRNAs per gene target (pooled in a single well), for a total of 1,312 gRNAs. Libraries are delivered as 100 μL of ready-to-use lentiviral particles per gene target with an average titer of 1X10⁸ TU/ml and are also available as glycerol stocks. Libraries are supplied in 96-well plates.

The Invitrogen LentiPool CRISPR library has the same gene coverage and is supplied as lentiviral pools containing four gRNAs per gene, delivered as ready-to-use lentivirus at high titer: >1 x 10⁸ TU/mL.

• Ready to use
• Glycerol stock
• LentiPool format

The broad array of cell surface proteins allows cells to receive information from, and react to, their environment. The gene targets within the Invitrogen LentiArray CRISPR Human Cell Surface Protein Library were selected using the most up-to-date genome databases, including the NCBI RefSeq database, and cross-referenced to the Gene Ontology Consortium (GO) database and/or the HUGO Gene Nomenclature Committee (HGNC). The gRNA designs for each target were then created using a proprietary gRNA design algorithm developed by the scientists at Thermo Fisher Scientific. The LentiArray CRISPR Human Cell Surface Protein Library targets 778 genes with up to four gRNAs per gene target (pooled in a single well), for a total of 3,112 gRNAs. Libraries are delivered as 100 μL of ready-to-use lentiviral particles per gene target with an average titer of 1X10⁸ TU/ml and are also available as glycerol stocks. Libraries are supplied in 96-well plates.

The Invitrogen LentiPool CRISPR library has the same gene coverage and is supplied as lentiviral pools containing four gRNAs per gene, delivered as ready-to-use lentivirus at high titer: >1 x 10⁸ TU/mL.

• Ready to use
• Glycerol stock
• LentiPool format

Proteases degrade proteins but are also integral signaling molecules, and the dysregulation of protease signaling pathways has been implicated in the development of cardiovascular disease, neurological disorders, cancer, and inflammatory disease. The gene targets within the Invitrogen™ LentiArray™ CRISPR Human Protease Library were selected using the most up-to-date genome databases, including the NCBI RefSeq database, and cross-referenced to the Gene Ontology Consortium (GO) database and/or the HUGO Gene Nomenclature Committee (HGNC). The gRNA designs for each target were then created using a proprietary gRNA design algorithm developed by the scientists at Thermo Fisher Scientific. The LentiArray CRISPR Human Protease Library targets 475 genes with up to four gRNAs per gene target (pooled in a single well), for a total of 1,900 gRNAs. Libraries are delivered as 100 μL of ready-to-use lentiviral particles per gene target with an average titer of 1X10⁸ TU/ml and are also available as glycerol stocks. Libraries are supplied in 96-well plates.

The Invitrogen LentiPool CRISPR library has the same gene coverage and is supplied as lentiviral pools containing four gRNAs per gene, delivered as ready-to-use lentivirus at high titer: >1 x 10⁸ TU/mL.

• Ready to use
• Glycerol stock
• LentiPool format

Tumor suppressors are negative regulators of cell cycle progression, and loss or mutation of tumor suppressor genes is often involved in the development of cancer. The gene targets within the Invitrogen LentiArray CRISPR Human Tumor Suppressor Library were selected using the most up-to-date genome databases, including the NCBI RefSeq database, and cross-referenced to the Gene Ontology Consortium (GO) database and/or the HUGO Gene Nomenclature Committee (HGNC) as well as the Tumor Suppressor Gene Database (TSGene). The gRNA designs for each target were then created using a proprietary gRNA design algorithm developed by the scientists at Thermo Fisher Scientific. The LentiArray CRISPR Human Tumor Suppressor Library targets 716 genes with up to four gRNAs per gene target (pooled in a single well), for a total of 2,864 gRNAs. Libraries are delivered as 100 μL of ready-to-use lentiviral particles per gene target with an average titer of 1X10⁸ TU/ml and are also available as glycerol stocks. Libraries are supplied in 96-well plates.

The Invitrogen LentiPool CRISPR library has the same gene coverage and is supplied as lentiviral pools containing four gRNAs per gene, delivered as ready-to-use lentivirus at high titer: >1 x 10⁸ TU/mL.

• Ready to use
• Glycerol stock
• LentiPool format

DNA surveillance proteins continuously monitor DNA integrity and activate cell cycle checkpoints and DNA repair pathways in response to DNA damage. The gene targets within the Invitrogen LentiArray CRISPR Human DNA Damage Response Library were selected using the most up-to-date genome databases, including the NCBI RefSeq database, and cross-referenced to the Gene Ontology Consortium (GO) database and/or the HUGO Gene Nomenclature Committee (HGNC). The gRNA designs for each target were then created using a proprietary gRNA design algorithm developed by the scientists at Thermo Fisher Scientific. The LentiArray CRISPR Human DNA Damage Response Library targets 516 genes with up to four gRNAs per gene target (pooled in a single well), for a total of 2,244 gRNAs . Libraries are delivered as 100 μL of ready-to-use lentiviral particles per gene target with an average titer of 1X10⁸ TU/ml and are also available as glycerol stocks. Libraries are supplied in 96-well plates.

The Invitrogen LentiPool CRISPR library has the same gene coverage and is supplied as lentiviral pools containing four gRNAs per gene, delivered as ready-to-use lentivirus at high titer: >1 x 10⁸ TU/mL.

• Ready to use
• Glycerol stock
• LentiPool format

Reversible phosphorylation is a central regulatory process in signal transduction pathways. Phosphatases dephosphorylate their target proteins, are integral to the regulation of the signaling pathways, and are increasingly being analyzed as potential therapeutic targets. The gene targets within the Invitrogen LentiArray Human Phosphatase CRISPR Library were selected using the most up-to-date genome databases, including the NCBI RefSeq database, and cross-referenced to the Gene Ontology Consortium (GO) database and/or the HUGO Gene Nomenclature Committee (HGNC). The gRNA designs for each target were then created using a proprietary gRNA design algorithm developed by the scientists at Thermo Fisher Scientific. The LentiArray CRISPR Human Phosphatase Library targets 288 genes with up to four gRNAs per gene target (pooled in a single well), for a total of 1,152 gRNAs. Libraries are delivered as 100 μL of ready-to-use lentiviral particles per gene target with an average titer of 1X10⁸ TU/ml and are also available as glycerol stocks. Libraries are supplied in 96-well plates.

The Invitrogen LentiPool CRISPR library has the same gene coverage and is supplied as lentiviral pools containing four gRNAs per gene, delivered as ready-to-use lentivirus at high titer: >1 x 10⁸ TU/mL.

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• Ready to use
• Glycerol stock
• LentiPool format

High quality controls play an integral role in the successful development and performance of high throughput screens. Don't forget to get your controls to help you optimize delivery conditions, maximize editing efficiency and establish hit selection criteria.