Evolved for choice and longevity—TaqMan SARS-CoV-2 2.0 assays
Choose from our two new advanced assays of Applied Biosystems TaqMan SARS-CoV-2 2.0 assays that deliver the same accuracy and reliability that our SARS-CoV-2 assays are known for, while offering the ability to accommodate multiple sample types and the choice to utilize either a standard or fast workflow. The standard workflow includes RNA extraction and traditional sample preparation, and the fast workflow is a fast, direct-to-PCR workflow with no RNA extraction required. You’ll also have confidence in your results knowing that our 2.0 assays include enhanced target redundancy to compensate for all SARS-CoV-2 mutations known now and those yet to be discovered in the future.
Features of the TaqMan SARS-CoV-2 2.0 assays include:
- Same accuracy and reliability you have grown to trust—an advanced assay design using multiple targets on orf1a, orf1b, and N genes (Figure 1) compensates for current and emerging SARS-CoV-2 mutations, helping provide continued confidence in results now and into the future
- Multiple workflow options—choose from two assays: one utilizes a standard workflow that includes RNA extraction (Figure 2), and the other utilizes a fast, direct-to-PCR workflow with no RNA extraction required (Figure 3)
- Affordable and scalable—add to your existing SARS-CoV-2 menu using your current real-time PCR instrumentation
Product details for TaqMan 2.0 assays:
| TaqMan SARS-CoV-2 RNase P Assay 2.0
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Description | Fast, direct-to-PCR, raw saliva solution using an evolved assay design to enable widespread, high-frequency testing (Figure 3) | An evolved SARS-CoV-2 assay design, with RNase P for human sample confirmation (Figure 2)
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Assay design | 3 genomic regions (orf1a, orf1b, and N genes) with 8 total targets (Figure 1) | ||
Internal control | RNase P | RNase P |
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Compatible sample types | Raw saliva collected with: | RNA extracted from nasopharyngeal swabs and anterior nasal swabs
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Recommended sample preparation | Lysis buffer (SalivaReady Solution, part of the TaqMan SARS-CoV-2 Fast PCR Combo Kit 2.0) | KingFisher Flex Purification System with MagMAX Viral/Pathogen II Nucleic Acid Isolation Mix | |
Recommended real-time PCR instruments | |||
Turnaround time (including sample prep) | ~ 2 hours | ~ 3 hours | |
Recommended software | QuantStudio Design & Analysis Software, RUO Edition 2.5 or higher | ||
Advanced 2.0 Assay Design
Recommended workflows
Figure 2. Recommended workflow for TaqMan SARS-CoV-2 RNase P Assay 2.0.
Figure 3. Simplified, direct-to-PCR workflow for TaqMan SARS-CoV-2 Fast PCR Combo Kit 2.0.
Analysis Software
Use QuantStudio Design & Analysis Software, RUO Edition 2.5 or higher.
Resources
For Research Use Only. Not for use in diagnostic procedures.