Invitrogen

p27 Kip1 Polyclonal Antibody

Advanced Verification

IP-mass spectrometry

1 Published Figure

6 References

View all (84) p27Kip1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

p27 Kip1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 8

p27 Kip1 Antibody (PA5-27188) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of p27 Kip1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: p27 Kip1 Polyclonal Antibody (Product # PA5 27188) diluted at 1:500. Red: phalloidin, a cytoskeleton marker. Scale bar = 10 µm.

p27 Kip1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

p27 Kip1 Antibody in Immunohistochemistry (Frozen) (IHC (F))

p27 Kip1 Antibody in Immunohistochemistry (IHC)

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Product Details

PA5-27188

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 2 publications 2 publications

Immunoprecipitation (IP)

1:100-1:500

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 55 and 198 of Human P27 Kip1

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.08 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2544664

Product Specific Information

Recommended positive controls: HeLa, MCF-7, MDA-MB-231, Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12.

Predicted reactivity: Mouse (80%), Dog (87%), Cat (87%), Pig (84%), Rhesus Monkey (98%), Bovine (84%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

p27 Kip1 functions as a negative regulator of G1 mouse expression and has been proposed to function as a possible mediator of TGFb induced G1 arrest. p27 Kip1 is an important regulator of cell cycle progression, a potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. p27 Kip1 forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. p27 Kip1 acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry. p27 Kip1 has a molecular weight of approximately 27kDa and the degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Bacterial protein MakA causes suppression of tumour cell proliferation via inhibition of PIP5K1α/Akt signalling.

Cell death & disease

Toh E,Baryalai P,Nadeem A,Aung KM,Chen S,Persson K,Persson JL,Uhlin BE,Wai SN

PA5-27188 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to indicate that MakA could affect eukaryotic cell signalling pathways involved in lipid biosynthesis.

Tue Dec 06 00:00:00 EST 2022

Senolysis-Based Elimination of Chemotherapy-Induced Senescent Breast Cancer Cells by Quercetin Derivative with Blocked Hydroxy Groups.

Cancers

Lewińska A,Przybylski P,Adamczyk-Grochala J,Błoniarz D,Litwinienko G,Wnuk M

PA5-27188 was used in Immunocytochemistry-immunofluorescence to study targeted elimination of senescent cells using synthesized quercetin derivatives which were tested for improved senolytic action against etoposide-induced senescent human normal mammary epithelial cells and triple-negative breast cancer cells in vitro.

Tue Jan 25 00:00:00 EST 2022

Human 3D cellular model of hypoxic brain injury of prematurity.

Nature medicine

Pașca AM,Park JY,Shin HW,Qi Q,Revah O,Krasnoff R,O'Hara R,Willsey AJ,Palmer TD,Pașca SP

PA5-27188 was used in Immunocytochemistry-immunoflourescence to anticipate that this human cellular platform will be valuable for studying the environmental and genetic factors underlying injury in the developing human brain.

Wed May 01 00:00:00 EDT 2019

CDKN1B/p27 is localized in mitochondria and improves respiration-dependent processes in the cardiovascular system-New mode of action for caffeine.

PLoS biology

Ale-Agha N,Goy C,Jakobs P,Spyridopoulos I,Gonnissen S,Dyballa-Rukes N,Aufenvenne K,von Ameln F,Zurek M,Spannbrucker T,Eckermann O,Jakob S,Gorressen S,Abrams M,Grandoch M,Fischer JW,Köhrer K,Deenen R,Unfried K,Altschmied J,Haendeler J

PA5-27188 was used in Immunohistochemistry-immunofluorescence to show that the cyclin-dependent kinase inhibitor 1B (CDKN1B)/p27, previously known as a cell cycle inhibitor, is also localized within mitochondria.

Fri Jun 01 00:00:00 EDT 2018

ESRP1 Mutations Cause Hearing Loss due to Defects in Alternative Splicing that Disrupt Cochlear Development.

Developmental cell

Rohacek AM,Bebee TW,Tilton RK,Radens CM,McDermott-Roe C,Peart N,Kaur M,Zaykaner M,Cieply B,Musunuru K,Barash Y,Germiller JA,Krantz ID,Carstens RP,Epstein DJ

PA5-27188 was used in Immunohistochemistry to elucidate the importance of mutations in ESRP1 as a cause of sensorineural hearing loss, and demonstrate the complex interplay between alternative splicing, inner ear development, and auditory function.

Mon Nov 06 00:00:00 EST 2017

Context-Dependent Functional Divergence of the Notch Ligands DLL1 and DLL4 In Vivo.

PLoS genetics

Preuße K,Tveriakhina L,Schuster-Gossler K,Gaspar C,Rosa AI,Henrique D,Gossler A,Stauber M

PA5-27188 was used in immunohistochemistry to determine tissue specific functions of DLL1 or DLL4 using transgenic mice.

Mon Jun 01 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: CDN1B; Cyclin-dependent kinase inhibitor 1B; cyclin-dependent kinase inhibitor 1B (p27, Kip1); Cyclin-dependent kinase inhibitor p27; Cyclin-dependent kinase inhibitor p27 Kip1; P27 CDKN1B; p27(KIP1); p27Kip1

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Gene Aliases: AA408329; AI843786; CDKN1B; CDKN4; KIP1; MEN1B; MEN4; p27; P27KIP1

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UniProt ID: (Human) P46527, (Mouse) P46414

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Entrez Gene ID: (Human) 1027, (Mouse) 12576

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Function(s)

cyclin-dependent protein serine/threonine kinase inhibitor activity transforming growth factor beta receptor, cytoplasmic mediator activity protein binding cysteine-type endopeptidase activator activity involved in apoptotic process protein phosphatase binding Hsp70 protein binding protein complex binding chaperone binding protein kinase inhibitor activity kinase inhibitor

Process(es)

regulation of cyclin-dependent protein serine/threonine kinase activity G1/S transition of mitotic cell cycle response to hypoxia potassium ion transport activation of cysteine-type endopeptidase activity involved in apoptotic process DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest cell cycle arrest Notch signaling pathway sensory perception of sound positive regulation of cell proliferation negative regulation of cell proliferation response to glucose positive regulation of cell death negative regulation of cell growth positive regulation of microtubule polymerization response to estradiol negative regulation of kinase activity negative regulation of phosphorylation response to drug negative regulation of apoptotic process response to amino acid response to peptide hormone positive regulation of protein catabolic process negative regulation of cyclin-dependent protein serine/threonine kinase activity positive regulation of cyclin-dependent protein serine/threonine kinase activity positive regulation of cell cycle negative regulation of transcription, DNA-templated negative regulation of mitotic cell cycle response to cadmium ion autophagic cell death inner ear development negative regulation of cellular component movement negative regulation of epithelial cell proliferation involved in prostate gland development cellular response to antibiotic cellular response to lithium ion cellular response to organic cyclic compound mitotic cell cycle arrest negative regulation of vascular smooth muscle cell proliferation cell cycle cell death regulation of cell proliferation negative regulation of epithelial cell proliferation

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p27 Kip1 Polyclonal Antibody

p27 Kip1 Antibody (PA5-27188) in ICC/IF

Product Details

PA5-27188

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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