Invitrogen

mCherry Recombinant Rat Monoclonal Antibody (16D7)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

View all (36) mCherry antibodies

Datasheet

Protocols

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Datasheet

Protocols

Questions & Answers

FIGURE: 1 / 4

mCherry Antibody (740003T) in WB

Western blot was performed using mCherry tag Recombinant Rat Monoclonal Antibody (16D7), Purified (Product # 740003T) by loading whole cell extracts of transiently transfected HEK-293E lysates: H3-mCherry, 5 µg (Lane 1), mCherry-GST, 0.125 µg (Lane 2), mCherry-GST, 0.25 µg (Lane 3), mCherry-GST, 0.5 µg (Lane 4), GST-mCherry, 0.125 µg (Lane 5), GST-mCherry, 0.25 µg (Lane 6), GST-mCherry, 0.5 µg (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitroce... View More

mCherry Antibody in Flow Cytometry (Flow)

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Product Details

740003T

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

Flow Cytometry (Flow)

0.25 µg/test

Product Specifications

Species Reactivity

Tag

Host/Isotype

Rat / IgG2a, kappa

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

16D7

Immunogen

Full-length protein mCherry

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_3090463

Product Specific Information

mCherry Tag recombinant rat monoclonal antibody specifically recognizes mCherry Tag at both C and N-terminus of proteins.

Recombinant rat monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Target Information

MCherry is a fluorophore (a fluorescent molecule) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cells components. mCherry is a monomeric fluorescent construct which absorbs at 587 nm and emits at 610 nm. It is resistant to photobleaching and is stable. It matures quickly, with a t 0. 5 of 15 minutes, allowing it to be visualized soon after translation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: mCherry

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